cDNA Clone Cullin2

• Plasmid: pCMV5-FLAG-1 Cullin2 K689R K719R
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG Cul2 K689R K719R
• Genbank: NM_003591
• Tag: FLAG
• Species: Human

Sequence of Insert

GGATCCATGTCTTTGAAACCAAGAGTAGTAGATTTTGATGAAACATGGAA
CAAACTTTTGACGACAATAAAAGCCGTGGTCATGTTGGAATACGTCGAAA
GAGCAACATGGAATGACCGTTTCTCAGATATCTATGCTTTATGTGTGGCC
TATCCTGAACCCCTTGGAGAAAGACTTTATACAGAAACTAAGATTTTTTT
GGAAAATCATGTTCGGCATTTGCATAAGAGAGTTTTGGAGTCAGAAGAAC
AAGTACTTGTTATGTATCATAGGTACTGGGAAGAATACAGCAAGGGTGCA
GACTATATGGACTGCTTATATAGGTATCTCAACACCCAGTTTATTAAAAA
GAATAAATTAACAGAAGCGGACCTTCAGTATGGCTATGGTGGTGTAGATA
TGAATGAACCACTTATGGAAATAGGAGAGCTAGCATTGGATATGTGGAGG
AAATTGATGGTTGAACCACTTCAGGCCATCCTTATCCGAATGCTGCTCCG
AGAAATCAAAAATGATCGTGGTGGAGAAGACCCAAACCAGAAAGTAATCC
ATGGGGTTATTAACTCCTTTGTTCATGTTGAACAGTATAAGAAAAAATTC
CCCTTAAAGTTTTATCAGGAAATTTTTGAGTCTCCCTTTCTGACTGAAAC
AGGAGAGTATTACAAACAAGAAGCTTCAAATTTATTACAAGAATCAAACT
GCTCACAGTATATGGAAAAGGTTCTAGGTAGATTAAAAGATGAAGAAATT
CGATGTCGAAAATACCTACATCCAAGTTCATATACTAAGGTGATTCATGA
ATGTCAACAACGAATGGTAGCAGACCACTTACAGTTTTTACATGCAGAAT
GTCATAATATAATTCGACAAGAGAAAAAAAATGACATGGCAAATATGTAC
GTCTTACTCCGTGCTGTGTCCACTGGTTTACCTCATATGATTCAGGAGCT
GCAAAACCACATCCATGATGAGGGCCTTCGAGCAACCAGCAACCTTACTC
AGGAAAACATGCCAACACTATTTGTGGAGTCAGTTTTGGAAGTGCATGGT
AAATTTGTTCAGCTTATCAACACTGTTTTGAATGGTGATCAGCATTTTAT
GAGTGCGTTGGATAAGGCCCTTACGTCAGTTGTAAATTACAGAGAACCTA
AGTCTGTTTGCAAAGCACCTGAACTGCTTGCTAAGTACTGTGACAACTTA
CTGAAGAAGTCAGCGAAAGGGATGACAGAGAATGAAGTGGAAGACAGGCT
CACGAGCTTCATCACAGTGTTCAAATACATTGATGACAAGGACGTCTTTC
AAAAGTTCTACGCAAGAATGCTGGCAAAACGTTTAATTCATGGGTTATCC
ATGTCTATGGACTCTGAAGAAGCCATGATCAACAAATTAAAGCAAGCCTG
TGGTTATGAGTTTACCAGCAAGCTACATCGGATGTATACAGATATGAGTG
TCAGCGCTGATCTCAACAATAAGTTCAACAATTTTATCAAAAACCAAGAC
ACAGTAATAGATTTGGGAATTAGTTTTCAAATATATGTTCTACAGGCTGG
TGCGTGGCCTCTTACTCAGGCTCCTTCATCTACGTTTGCAATTCCCCAGG
AATTAGAAAAAAGTGTACAGATGTTTGAATTATTTTATAGCCAACATTTC
AGTGGAAGGAAACTTACATGGTTACATTATCTGTGTACAGGTGAAGTTAA
AATGAACTATTTGGGCAAACCATATGTAGCCATGGTTACAACATACCAAA
TGGCAGTTCTTCTTGCCTTTAACAACAGTGAAACTGTCAGTTATAAAGAG
CTTCAGGACAGCACTCAGATGAATGAAAAGGAACTGACAAAAACAATCAA
ATCATTACTTGATGTGAAAATGATTAACCATGATTCAGAAAAGGAAGATA
TTGATGCAGAATCTTCGTTTTCATTAAATATGAACTTTAGCAGTAAAAGA
ACAAAATTTAAAATTACTACATCAATGCAGAAAGACACACCACAAGAAAT
GGAGCAGACTAGAAGTGCAGTTGATGAGGACCGGAAAATGTATCTCCAAG
CTGCTATAGTTCGTATCATGAGAGCACGAAAAGTGCTTCGGCACAATGCC
CTTATTCAAGAGGTGATTAGCCAGTCAAGAGCTAGGTTTAATCCCAGTAT
CAGCATGATTAGGAAGTGTATTGAAGTTCTGATAGACAAACAATACATAG
AACGCAGCCAGGCGTCGGCAGATGAATACAGCTACGTCGCGTGAGCGGCC
GC

Amino Acid Sequence

DYKDDDDKGSMSLKPRVVDFDETWNKLLTTIKAVVMLEYVERATWNDRFSDIYALCVAYPEPLGERLYTETKIFLENHVRHLHKRVLESEEQVLVMYHRYWEEYSKGADYMDCLYRYLNTQFIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDMWRKLMVEPLQAILIRMLLREIKNDRGGEDPNQKVIHGVINSFVHVEQYKKKFPLKFYQEIFESPFLTETGEYYKQEASNLLQESNCSQYMEKVLGRLKDEEIRCRKYLHPSSYTKVIHECQQRMVADHLQFLHAECHNIIRQEKKNDMANMYVLLRAVSTGLPHMIQELQNHIHDEGLRATSNLTQENMPTLFVESVLEVHGKFVQLINTVLNGDQHFMSALDKALTSVVNYREPKSVCKAPELLAKYCDNLLKKSAKGMTENEVEDRLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSMDSEEAMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKNQDTVIDLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYSQHFSGRKLTWLHYLCTGEVKMNYLGKPYVAMVTTYQMAVLLAFNNSETVSYKELQDSTQMNEKELTKTIKSLLDVKMINHDSEKEDIDAESSFSLNMNFSSKRTKFKITTSMQKDTPQEMEQTRSAVDEDRKMYLQAAIVRIMRARKVLRHNALIQEVISQSRARFNPSISMIRKCIEVLIDKQYIERSQASADEYSYVA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU20289
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 88179.2 Daltons