cDNA Clone HDAC2

• Plasmid: pEGFP-C1-HDAC2
• Parent Plasmid: pEGFPC1
• Expressed: GFP-HDAC2
• Genbank: NM_001527
• Tag: GFP
• Species: Human

Sequence of Insert

ggatccATGGCGTACAGTCAAGGAGGCGGCAAAAAAAAAGTCTGCTACTA
CTACGACGGTGATATTGGAAATTATTATTATGGACAGGGTCATCCCATGA
AGCCTCATAGAATCCGCATGACCCATAACTTGCTGTTAAATTATGGCTTA
TACAGAAAAATGGAAATATATAGGCCCCATAAAGCCACTGCCGAAGAAAT
GACAAAATATCACAGTGATGAGTATATCAAATTTCTACGGTCAATAAGAC
CAGATAACATGTCTGAGTATAGTAAGCAGATGCAGAGATTTAATGTTGGA
GAAGATTGTCCAGTGTTTGATGGACTCTTTGAGTTTTGTCAGCTCTCAAC
TGGCGGTTCAGTTGCTGGAGCTGTGAAGTTAAACCGACAACAGACTGATA
TGGCTGTTAATTGGGCTGGAGGATTACATCATGCTAAGAAATCAGAAGCA
TCAGGATTCTGTTACGTTAATGATATTGTGCTTGCCATCCTTGAATTACT
AAAGTATCATCAGAGAGTCTTATATATTGATATAGATATTCATCATGGTG
ATGGTGTTGAAGAAGCTTTTTATACAACAGATCGTGTAATGACGGTATCA
TTCCATAAATATGGGGAATACTTTCCTGGCACAGGAGACTTGAGGGATAT
TGGTGCTGGAAAAGGCAAATACTATGCTGTCAATTTTCCAATGAGAGATG
GTATAGATGATGAGTCATATGGGCAGATATTTAAGCCTATTATCTCAAAG
GTGATGGAGATGTATCAACCTAGTGCTGTGGTATTACAGTGTGGTGCAGA
CTCATTATCTGGTGATAGACTGGGTTGTTTCAATCTAACAGTCAAAGGTC
ATGCTAAATGTGTAGAAGTTGTAAAAACTTTTAACTTACCATTACTGATG
CTTGGAGGAGGTGGCTACACAATCCGTAATGTTGCTCGATGTTGGACATA
TGAGACTGCAGTTGCCCTTGATTGTGAGATTCCCAATGAGTTGCCATATA
ATGATTACTTTGAGTATTTTGGACCAGACTTCAAACTGCATATTAGTCCT
TCAAACATGACAAACCAGAACACTCCAGAATATATGGAAAAGATAAAACA
GCGTTTGTTTGAAAATTTGCGCATGTTACCTCATGCACCTGGTGTCCAGA
TGCAAGCTATTCCAGAAGATGCTGTTCATGAAGACAGTGGAGATGAAGAT
GGAGAAGATCCAGACAAGAGAATTTCTATTCGAGCATCAGACAAGCGGAT
AGCTTGTGATGAAGAATTCTCAGATTCTGAGGATGAAGGAGAAGGAGGTC
GAAGAAATGTGGCTGATCATAAGAAAGGAGCAAAGAAAGCTAGAATTGAA
GAAGATAAGAAAGAAACAGAGGACAAAAAAACAGACGTTAAGGAAGAAGA
TAAATCCAAGGACAACAGTGGTGAAAAAACAGATACCAAAGGAACCAAAT
CAGAACAGCTCAGCAACCCCTGAggatcc

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLRSRAQASN
SAVDGTAGPGSMAYSQGGGKKKVCYYYDGDIGNYYYGQGHPMKPHRIRMT
HNLLLNYGLYRKMEIYRPHKATAEEMTKYHSDEYIKFLRSIRPDNMSEYS
KQMQRFNVGEDCPVFDGLFEFCQLSTGGSVAGAVKLNRQQTDMAVNWAGG
LHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFY
TTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPMRDGIDDESYG
QIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVV
KTFNLPLLMLGGGGYTIRNVARCWTYETAVALDCEIPNELPYNDYFEYFG
PDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDA
VHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHK
KGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSNP

• Vector Type: Mammalian
• Antibiotic: Kan
• DU Number: DU7139
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Construct may rearrange in DH5∝ cells, but higher stability is obtained in the E.coli strain SCS110 (Stratagene).; The cloned protein sequence is identical to database accessions CAI15363, CAI14207, Q92769, AAH31055, AAX29896, which differ from the published NM_001527 sequence at five sites (Q93H, R94I, V103A, S146Y and R230C).