cDNA Clone AS160

• Synonyms: TBC1D4
• Plasmid: pCMV5-HA AS160A S751A
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-AS160A S751A
• Genbank: NM_014832
• Tag: HA
• Species: Human

Sequence of Insert

ggatccATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCC
CCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGCCCGGCCCCGGGAAGCCAA
GCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGACCAC
AGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCG
CAGCCAGAAGCCCGAGGCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGG
TGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCGTCCCCGCGCCGGGC
GCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGC
GGTATTCATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACA
ACAGCCACGACCTCACCTACTTTGCCTACCTGATCAAGGCGCAGCCCGAC
GACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACCCCAG
CCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCA
TGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCCTTTTACAAC
TCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGTGACCCACAA
GAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGC
ACGAACAGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGTCCGGACCCA
GGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTCCCCCGGAGA
CTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTACCTG
CCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAG
CGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCG
GTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGGGCAGCCAGA
AATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCC
TCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGA
GATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAGAAAAGAATT
TTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTGGATCACTTT
GGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTG
TTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGGTAATGCTGA
CTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGTGCCAAGACG
CAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTG
TGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGA
GGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATCTTTGAAAGA
GTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTGTGAT
TTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACA
TCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCCAGAAAATGCA
ACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATAAAGCTAAGAG
ATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAA
TGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTT
GCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGGGACACCGCC
AGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGATTCCG
ACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCA
AGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCG
TTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGTGATGGAGAAG
GGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTGGGA
GGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATTTTCCT
CAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGCAAG
ATGGATTGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTCTCTCCAACC
ATGGAGGAGGAACCGCTGGTTATATTCCTGTCTGGGGAGGATGACCCAGA
AAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCTTGTGGAGAA
AAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAACCAG
AAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGA
CTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAACTTGGGATA
AGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATGGAAGATATT
CATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAATTTG
GCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGATTGCCTAATAAAC
AACAGCCTCCTGACATATCCTATAAGGAACTTTTGAGGCAGCTCACTGCT
CAGCAGCATGCGATTCTTGTGGATTTAGGAAGGACGTTTCCTACTCACCC
TTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCC
TGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGATACTGTCAGGGGATC
AGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCAAGCCTT
TGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAGTACA
GACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTGTCCAGGCTC
CTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGAAAATGAAAT
CAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTC
AGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAG
GGAACTGAAGTTATATTCAAGGTTGCACTCAGCCTACTGAGCAGCCAAGA
GACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGTTTCTTA
AAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATTACC
CAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCATGCCTATGAGGTGGA
ATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCATATTCCTGTGAGG
ATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGA
CAAAACATGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCA
GGCCTTGGAATCAAACCTGGAAAATCTTTTGACGAGAGAGACCAAAATGA
AGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAAAAGACA
GTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGCCAATTGTGA
CCTGTTGCTGAGAGACCTAAACTGCAACCCTAACAACAAAGCCAAGATAG
GAAATAAGCCATAAggatcc

Amino Acid Sequence

MYPYDVPDYAGSMEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRL
WYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSA
PFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTY
FAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKP
SKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLK
IQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPA
LTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRR
HASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISPC
SQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFS
TAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLT
DNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPAT
ISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGS
VDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRR
RAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSDGEGRKRTSA
TCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNE
LLPLSPLSPTMEEEPLVIFLSGEDDPEKIEERKKSKELRSLWRKAIHQQI
LLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCR
AKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDIS
YKELLRQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLL
DKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSL
QIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFV
ARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDM
NTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEK
LERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTL
EQEKMAYQKTVEQLRKLLPADALANCDLLLRDLNCNPNNKAKIGNKP

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7105
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Protein sequence has the following mutations compared to NM_014832: M1K, S387P and K958R; The cloned sequence represents a splice variant lacking amino acids 679-741. The majority of ESTs in the NCBI database represent this form.; A further single amino acid deletion (G865) is also well documented in the NCBI EST database, e.g.; gi|33251358|gb|CF135914.1|, ; gi|32238979|gb|CD708349.1|, ; gi|32046370|emb|BX508803.1|.; ; Estimated MW of expressed protein : 140903 Daltons