cDNA Clone
AS160

Synonyms
TBC1D4
Plasmid
pCMV5-HA AS160A del 52-154 S318A
Parent Plasmid
pCMV5 HA2
Expressed
HA-AS160A del 52-154 S318A
Genbank
NM_014832
Tag
HA
Species
Human
Sequence of Insert
gcggccgcgaattcactagtgattAAGGAGCCGCCCAGCTGCATTCAGGA
TGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAGCTCAGC
CCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGG
GGGTCGTGCCTGGACCACAGGACCACGGCCTGCCACGTTTTCCGCGCCAC
AGACCCCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTA
AAGCGGCCATGAAAGAGGATGCCAAACCCAGCAAAGATAATGAGGACGCC
TTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTGACCGT
GACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGT
TCAGCCTGCACGAACAGCAGCGCCTGAAGATCCAAGGCGAGCAGCGCGGT
CCGGACCCAGGAGAGGACCTGGCTGACTTGGAGGTGGTGGTGCCCGGGTC
CCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTG
GCTTACCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGC
TTCCCTGAGCGGATTTTGGAAGATTCTGGCTTTGATGAGCAGCAGGAGTT
TCGGTCTCGGTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCACGAGG
GCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCAC
GTCCAGCCCTCGGACTCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGG
GCGATTTGAGATTAACCTTATCAGTCCAGACACTAAATCAGTTGTGCTAG
AAAAGAATTTTAAAGATATCTCCCCTTGTTCTCAGGGTATAAAGCATGTG
GATCACTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCA
GTATATTTGTTATGTATTCCAGTGTGCCAGCGAATCTCTGGTTGATGAGG
TAATGCTGACTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGCAGAGT
GCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCA
TAAGCTCTGTGAAAGGATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGG
TGATACAGAGGCATCTCTCATCACTGACAGATAATGAGCAAGCTGACATC
TTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGA
ACTTGTGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACAC
ACGTGCACATCGGGGAAGGCCCTGCTACTATTTCAAATAGTACAATCCCA
GAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAAAATAA
AGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAG
CTAACAGAATGAGAGGTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCC
AACAGTCTTGCTTCAGAGAAGGACTACTCACCAGGGGATTCTCCACCAGG
GACACCGCCAGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAG
AGGATTCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCAC
CCACCTTCAAGCACAAAGAGAAAGCTGAATTTGCAGGATGGGAGGGCTCA
GGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTGCAGTG
ATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTA
AGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCG
CATTTTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGC
AACAGCAAGATGGATTGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTC
TCTCCAACCATGGAGGAGGAACCGCTGGTTATATTCCTGTCTGGGGAGGA
TGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGGAGCT
TGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAA
GAAAACCAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGT
TAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAA
CTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATG
GAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGG
AGAAATTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGATTGC
CTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTGAGGCAG
CTCACTGCTCAGCAGCATGCGATTCTTGTGGATTTAGGAAGGACGTTTCC
TACTCACCCTTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGT
TTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAGAAGTGGGATACTGT
CAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGA
GCAAGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCA
AGCAGTACAGACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTG
TCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCTTGAAGA
AAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGT
TTGCCTCTCAGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGATATTATT
TTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCACTCAGCCTACTGAG
CAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTG
AGTTTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAA
ATTATTACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCATGCCTA
TGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCATATT
CCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAA
CTGAAAAGACAAAACATGGACCTCCTAGAAAAATTACAGGTAGCTCATAC
TAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCTTTTGACGAGAGAGA
CCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTAT
CAAAAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGC
CAATTGTGACCTGTTGCTGAGAGACCTAAACTGCAACCCTAACAACAAAG
CCAAGATAGGAAATAAGCCATAAggatccgcaatcgaattcccgcggccg
c
Amino Acid Sequence
MYPYDVPDYAGIPGIPGSTRAAANSLVIKEPPSCIQDEPFPHPLEPEPGV
SAQPGPGKPSDKRFRLWYVGGSCLDHRTTACHVFRATDPSQVPDVISSIR
QLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDC
MEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTD
THLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSAVTGVQRR
VHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKS
VVLEKNFKDISPCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESL
VDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPR
AKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAK
QKTHVHIGEGPATISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIF
SRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQT
FPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSE
QCSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSP
SAMQQQDGLDRNELLPLSPLSPTMEEEPLVIFLSGEDDPEKIEERKKSKE
LRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKE
VLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLR
HRLPNKQQPPDISYKELLRQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQ
LSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDL
GFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWF
LTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFE
NIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQE
SSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLL
TRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALANCDLLLRDLNCNP
NNKAKIGNKP
Vector Type
Mammalian
Antibiotic
Amp
DU Number
DU7094
Location
PPU
MTA
Notes
Protein sequence has the following mutations compared to NM_014832 :
M1K, S387P and K958R.
• Amino acids 52 to 154 were deleted during RT-PCR.
• The cloned sequence represents a splice variant lacking amino acids 679-741. However, the majority of ESTs in the NCBI database represent this variant.
A further single amino acid deletion (G865) is also well documented in the NCBI EST database, e.g.
gi|33251358|gb|CF135914.1|,
gi|32238979|gb|CD708349.1|.
Price
£125.00
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