cDNA Clone GYS1

• Plasmid: pEBG6P1-GYS1
• Parent Plasmid: pEBG6P
• Expressed: GST-GYS1
• Genbank: NM_002103
• Tag: GST

Sequence of Insert

actagtCCTTTAAACCGCACTTTGTCCATGTCCTCACTGCCAGGACTGGA
GGACTGGGAGGATGAATTCGACCTGGAGAACGCAGTGCTCTTCGAAGTGG
CCTGGGAGGTGGCTAACAAGGTGGGTGGCATCTACACGGTGCTGCAGACG
AAGGCGAAGGTGACAGGGGACGAATGGGGCGACAACTACTTCCTGGTGGG
GCCGTACACGGAGCAGGGCGTGAGGACCCAGGTGGAACTGCTGGAGGCCC
CCACCCCGGCCCTGAAGAGGACACTGGATTCCATGAACAGCAAGGGCTGC
AAGGTGTATTTCGGGCGCTGGCTGATCGAGGGAGGCCCTCTGGTGGTGCT
CCTGGACGTGGGTGCCTCAGCTTGGGCCCTGGAGCGCTGGAAGGGAGAGC
TCTGGGATACCTGCAACATCGGAGTGCCGTGGTACGACCGCGAGGCCAAC
GACGCTGTCCTCTTTGGCTTTCTGACCACCTGGTTCCTGGGTGAGTTCCT
GGCACAGAGTGAGGAGAAGCCACATGTGGTTGCTCACTTCCATGAGTGGT
TGGCAGGCGTTGGACTCTGCCTGTGTCGTGCCCGGCGACTGCCTGTAGCA
ACCATCTTCACCACCCATGCCACGCTGCTGGGGCGCTACCTGTGTGCCGG
TGCCGTGGACTTCTACAACAACCTGGAGAACTTCAACGTGGACAAGGAAG
CAGGGGAGAGGCAGATCTACCACCGATACTGCATGGAAAGGGCGGCAGCC
CACTGCGCTCACGTCTTCACTACTGTGTCCCAGATCACCGCCATCGAGGC
ACAGCACTTGCTCAAGAGGAAACCAGATATTGTGACCCCCAATGGGCTGA
ATGTGAAGAAGTTTTCTGCCATGCATGAGTTCCAGAACCTCCATGCTCAG
AGCAAGGCTCGAATCCAGGAGTTTGTGCGGGGCCATTTTTATGGGCATCT
GGACTTCAACTTGGACAAGACCTTATACTTCTTTATCGCCGGCCGCTATG
AGTTCTCCAACAAGGGTGCTGACGTCTTTCTGGAGGCATTGGCTCGGCTC
AACTATCTGCTCAGAGTGAACGGCAGCGAGCAGACAGTGGTTGCCTTCTT
CATCATGCCAGCGCGGACCAACAATTTCAACGTGGAAACCCTCAAAGGCC
AAGCTGTGCGCAAACAGCTTTGGGACACGGCCAACACGGTGAAGGAAAAG
TTCGGGAGGAAGCTTTATGAATCCTTACTGGTTGGGAGCCTTCCCGACAT
GAACAAGATGCTGGATAAGGAAGACTTCACTATGATGAAGAGAGCCATCT
TTGCAACGCAGCGGCAGTCTTTCCCCCCTGTGTGCACCCACAATATGCTG
GATGACTCCTCAGACCCCATCCTGACCACCATCCGCCGAATCGGCCTCTT
CAATAGCAGTGCCGACAGGGTGAAGGTGATTTTCCACCCGGAGTTCCTCT
CCTCCACAAGCCCCCTGCTCCCTGTGGACTATGAGGAGTTTGTCCGTGGC
TGTCACCTTGGAGTCTTCCCCTCCTACTATGAGCCTTGGGGCTACACACC
GGCTGAGTGCACGGTTATGGGAATCCCCAGTATCTCCACCAACCTCTCCG
GCTTCGGCTGCTTCATGGAGGAACACATCGCAGACCCCTCAGCTTACGGT
ATCTACATTCTTGACCGGCGGTTCCGCAGCCTGGATGATTCCTGCTCGCA
GCTCACCTCCTTCCTCTACAGTTTCTGTCAGCAGAGCCGGCGGCAGCGTA
TCATCCAGCGGAACCGCACGGAGCGCCTCTCCGACCTTCTGGACTGGAAA
TACCTAGGCCGGTACTATATGTCTGCGCGCCACATGGCGCTGTCCAAGGC
CTTTCCAGAGCACTTCACCTACGAGCCCAACGAGGCGGATGCGGCCCAGG
GGTACCGCTACCCACGGCCAGCCTCGGTGCCACCGTCGCCCTCGCTGTCA
CGACACTCCAGCCCGCACCAGAGTGAGGATGAGGAGGATCCCCGGAACGG
GCCGCTGGAGGAAGACGGCGAGCGCTACGATGAGGACGAGGAGGCCGCCA
AGGACCGGCGCAACATCCGTGCACCAGAGTGGCCGCGCCGAGCGTCCTGC
ACCTCCTCCACCAGCGGCAGCAAGCGCAACTCTGTGGACACGGCCACCTC
CAGCTCACTCAGCACCCCGAGCGAGCCCCTCAGCCCCACCAGCTCCCTGG
GCGAGGAGCGTAACTAAactagt

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSPLNRTLSMSSL
PGLEDWEDEFDLENAVLFEVAWEVANKVGGIYTVLQTKAKVTGDEWGDNY
FLVGPYTEQGVRTQVELLEAPTPALKRTLDSMNSKGCKVYFGRWLIEGGP
LVVLLDVGASAWALERWKGELWDTCNIGVPWYDREANDAVLFGFLTTWFL
GEFLAQSEEKPHVVAHFHEWLAGVGLCLCRARRLPVATIFTTHATLLGRY
LCAGAVDFYNNLENFNVDKEAGERQIYHRYCMERAAAHCAHVFTTVSQIT
AIEAQHLLKRKPDIVTPNGLNVKKFSAMHEFQNLHAQSKARIQEFVRGHF
YGHLDFNLDKTLYFFIAGRYEFSNKGADVFLEALARLNYLLRVNGSEQTV
VAFFIMPARTNNFNVETLKGQAVRKQLWDTANTVKEKFGRKLYESLLVGS
LPDMNKMLDKEDFTMMKRAIFATQRQSFPPVCTHNMLDDSSDPILTTIRR
IGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPW
GYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDD
SCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMA
LSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEED
PRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSVD
TATSSSLSTPSEPLSPTSSLGEERN

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7037
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid to express human muscle glycogene synthase (GYS1) with N-terminal GST (contains precission protease site); ; Estimated MW of expressed protein : 111272 Daltons