cDNA Clone CYFIP1

• Plasmid: pCMV5-HA CYFIP1
• Parent Plasmid: pCMV5 HA1
• Expressed: HA-CYFIP1
• Genbank: NP_055423.[gi: 24307969]
• Tag: HA
• Species: Human

Sequence of Insert

gcggccgcgggaattcgattgcactagtATGGCGGCCCAGGTGACTCTGGAGGACGCGCTGTCCAACGTGGACCTCCTGGAGGAGCTGCCCCTGCCCGACCAGCAGCCCTGCATCGAGCCCCCGCCATCCTCGCTGCTCTACCAGCCAAATTTCAACACTAACTTTGAAGACAGAAATGCATTTGTTACTGGCATCGCAAGATACATTGAACAAGCCACCGTCCACTCTAGCATGAACGAGATGCTGGAGGAGGGCCAAGAATATGCTGTCATGCTGTACACCTGGAGGAGCTGCTCCCGGGCCATCCCACAGGTGAAATGTAACGAGCAGCCTAACAGAGTGGAAATCTACGAGAAAACCGTGGAGGTTCTGGAGCCTGAGGTCACAAAACTGATGAATTTCATGTACTTCCAGAGAAATGCCATTGAGCGTTTCTGCGGGGAAGTGAGGCGCCTGTGCCATGCCGAGAGGAGGAAGGACTTCGTGTCAGAAGCCTACCTGATCACACTGGGCAAATTCATCAACATGTTCGCTGTGCTGGACGAGCTGAAGAACATGAAGTGCAGTGTGAAGAACGACCACTCAGCGTACAAGAGGGCCGCTCAGTTTTTACGTAAAATGGCAGATCCACAGTCCATCCAGGAATCGCAGAATCTGTCCATGTTCCTGGCCAATCATAACAAGATCACACAGTCTCTGCAGCAGCAGCTCGAAGTGATTTCTGGCTACGAAGAGCTCCTGGCAGATATTGTGAATCTGTGTGTGGATTACTACGAGAACAGGATGTATTTGACGCCCAGTGAGAAACACATGCTTCTCAAAGTCATGGGATTTGGTCTGTACCTGATGGATGGGAGTGTCAGTAACATCTATAAGTTGGATGCCAAGAAAAGAATAAACTTATCCAAAATCGACAAGTACTTCAAGCAACTCCAGGTGGTTCCGCTATTTGGGGACATGCAAATAGAACTGGCAAGATATATCAAGACCAGCGCCCACTACGAGGAAAATAAATCTCGATGGACGTGCACATCCTCCGGCAGCAGCCCTCAGTACAACATCTGCGAGCAGATGATCCAGATCCGCGAGGACCACATGCGCTTCATTTCGGAGCTGGCGCGCTACAGCAACAGCGAGGTGGTCACGGGCTCGGGCCGCCAGGAGGCCCAGAAGACGGACGCGGAGTACCGCAAGCTCTTCGACCTGGCGCTGCAGGGCCTGCAGCTGTTGTCGCAGTGGAGCGCGCACGTGATGGAAGTGTATTCCTGGAAGCTTGTGCACCCCACCGACAAGTACTCCAACAAGGACTGCCCCGACAGCGCTGAAGAGTACGAGCGTGCCACGCGCTACAACTACACCAGCGAGGAGAAGTTTGCCCTAGTGGAGGTGATCGCCATGATCAAAGGCCTGCAGGTGCTGATGGGCAG
GATGGAGAGCGTGTTCAACCACGCCATCCGGCACACCGTCTATGCCGCACTGCAGGACTTCTCCCAGGTGACCCTTAGGGAGCCGCTGCGGCAGGCCATCAAGAAGAAGAAGAACGTCATCCAGAGTGTCCTGCAGGCCATCAGGAAGACCGTGTGTGACTGGGAGACGGGGCATGAGCCCTTCAATGACCCAGCCTTGCGGGGCGAGAAGGACCCCAAGAGCGGCTTCGACATAAAAGTACCACGCCGCGCCGTGGGACCCTCCAGCACTCAGCTTTACATGGTGAGAACCATGCTAGAGTCCCTCATTGCAGACAAAAGTGGTTCCAAGAAAACCTTGAGAAGTAGCCTTGAGGGGCCCACCATATTGGACATAGAAAAATTTCATCGAGAGTCATTCTTCTACACTCACTTGATAAATTTCAGTGAAACGCTGCAGCAGTGCTGTGACCTTTCGCAGCTGTGGTTCCGAGAGTTCTTCCTGGAGCTGACCATGGGCAGGAGGATCCAGTTCCCCATTGAGATGTCGATGCCCTGGATCCTGACGGACCACATCCTGGAGACCAAGGAGGCATCGATGATGGAGTACGTGCTCTACTCCCTGGACCTGTACAATGACAGCGCCCACTACGCGCTCACCAGGTTCAACAAGCAGTTCCTGTACGACGAAATTGAGGCCGAGGTGAATCTATGTTTTGACCAATTTGTTTACAAGCTAGCAGACCAGATATTTGCCTATTATAAGGTTATGGCAGGAAGTTTGCTTCTTGATAAACGGTTACGATCAGAATGCAAGAATCAGGGAGCCACGATCCACCTCCCGCCGTCTAACCGCTACGAGACGCTGCTGAAGCAGAGGCATGTGCAGCTCCTCGGCAGATCAATAGACCTCAATCGTCTGATCACCCAGCGCGTCTCAGCAGCCATGTATAAGTCCCTAGAACTGGCGATTGGACGATTTGAAAGTGAAGATTTGACCTCCATAGTTGAGCTGGATGGCCTGTTGGAAATCAACCGCATGACCCACAAGCTGCTGAGCCGGTACCTGACGCTGGACGGCTTCGACGCCATGTTCCGGGAGGCCAACCACAACGTGTCAGCGCCCTACGGGAGGATCACCCTGCACGTCTTCTGGGAGCTCAACTATGACTTCCTGCCCAACTACTGCTACAACGGCTCTACCAACCGGTTTGTTCGGACAGTGTTACCATTTTCTCAGGAATTTCAAAGAGATAAGCAGCCTAATGCACAGCCTCAGTATCTGCATGGATCCAAGGCTTTGAACTTGGCCTACTCCAGCATTTACGGCAGCTACCGGAACTTCGTGGGACCTCCACACTTTCAAGTCATCTGCCGGCTTCTCGGCTACCAGGGTATCGCCGTGGTCATGGAGGAGCTGCTGAAGGTCGTCAAGAGCCTGCTGCAAGGCACAATCCTGCAGTACGTGAAGACGCTGATGGAGGTGATGCCCAAGATCTGCCGCCTGCCCCGGCACGAGTACGGCTCTCCTGGTATCCTGGAGTTCTTCCACCACCAGCTGAAGGACATCGTGGAGTACGCAGAGCTGAAGACGGTGTGCTTCCAGAACCTGCGGGAGGTGGGGAACGCCATCCTCTTCTGCCTGCTCATCGAGCAGAGCCTGTCTTTAGAAGAAGTGTGTGACCTGCTGCACGCGGCTCCTTTCCAGAACATCTTGCCGCGAGTCCATGTGAAAGAGGGGGAGAGACTTGATGCCAAAATGAAAAGACTAGAATCAAAGTACGCCCCGCTGCATCTTGTCCCACTGATTGAAAGACTGGGGACCCCTCAGCAAATTGCCATCGCAAGAGAGGGGGACCTGCTGACAAAGGAGCGCCTCTGCTGCGGCCTGTCCATGTTTGAGGTCATCCTGACACGGATCCGGAGCTTTCTGGATGACCCCATCTGGCGCGGGCCTCTGCCCAGCAATGGGGTCATGCATGTGGACGAGTGTGTGGAGTTTCACAGACTGTGGAGTGCCATGCAGTTTGTCTACTGCATTCCCGTGGGGACACACGAGTTCACAGTCGAGCAGTGCTTTGGTGATGGGCTACACTGGGCTGGCTGTATGATCATCGTACTTCTTGGGCAGCAGCGGCGTTTTGCTGTGCTGGATTTCTGCTACCATCTACTTAAAGTCCAGAAACATGATGGCAAAGATGAGATTATTAAAAATGTGCCTTTGAAGAAGATGGTGGAGAGAATTCGCAAGTTCCAGATTCTCAATGATGAGATCATCACCATCCTGGATAAGTACCTGAAGTCAGGCGACGGGGAGGGCACGCCAGTGGAGCATGTGCGCTGCTTCCAGCCGCCCATCCACCAGTCCCTCGCCAGCAGCTGAgcggccgc

Amino Acid Sequence

MYPYDVPDYAGSPEFPGRLERPREFDCTSMAAQVTLEDALSNVDLLEELP
LPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNE
MLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTK
LMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVL
DELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKIT
QSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGL
YLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKT
SAHYEENKSRWTCTSSGSSPQYNICEQMIQIREDHMRFISELARYSNSEV
VTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTD
KYSNKDCPDSAEEYERATRYNYTSEEKFALVEVIAMIKGLQVLMGRMESV
FNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDW
ETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYMVRTMLESLIA
DKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQL
WFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLY
NDSAHYALTRFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSL
LLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQR
VSAAMYKSLELAIGRFESEDLTSIVELDGLLEINRMTHKLLSRYLTLDGF
DAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPF
SQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLL
GYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPG
ILEFFHHQLKDIVEYAELKTVCFQNLREVGNAILFCLLIEQSLSLEEVCD
LLHAAPFQNILPRVHVKEGERLDAKMKRLESKYAPLHLVPLIERLGTPQQ
IAIAREGDLLTKERLCCGLSMFEVILTRIRSFLDDPIWRGPLPSNGVMHV
DECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQ
RRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITI
LDKYLKSGDGEGTPVEHVRCFQPPIHQSLASS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU7036
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 148556 Daltons