cDNA Clone NCC (mouse)

• Synonyms: solute carrier family 12 member 3 isoform 2, Slc12a3,
• Plasmid: pCMV5-FLAG-1 NCC (mouse)
• Parent Plasmid: pCMV5 FLAG1
• Expressed: FLAG-NCC (mouse)
• Genbank: NP_062288.2
• Tag: FLAG
• Species: Mouse

Sequence of Insert

gaattcatggcagagctccccgtgacagagctgcctggcgatgctctgtgcagtgggcgcttcaccatcagcacactgatgggaggcgatgagcccccac
cagctgcctgtgacagcagccagcccagccacttaacacacggcagcaccttatacatgcgcaccttcggttacaacaccatagacgtggtgccggccta
cgaacactatgctaacagcgctctgcccggtgagccccggaaggtgcggcccaccctggctgacctgcattcattcctcaaggaaggtagccacctgcat
gccctggcctttgatggacggcaaggtcgtgagctgactgatggacttgtggaagatgagacaggcaccaacagtgagaagagccctggagaacctgttc
gcttcggctgggtcaagggggtgatgatccgttgcatgctcaacatctggggagtgatcctctacctgcgactcccctggattacggcgcaggcgggcat
cgtgctgacttggctcatcatcctgctgtcggtcatggtgacatccatcaccggcctctccatctccgccatctctaccaatggcaaggtcaagtcgggt
ggcacctatttccttatttcccggagtctgggaccagagctgggaggctccattggcctcatcttcgcctttgccaatgctgtgggtgtggccatgcaca
ctgtgggctttgcagaaaccgtaagggacctactgcaggagtatggcacacccatcgtagaccccatcaatgacatccgcatcatcggtgtggtcacggt
cactgtgctgctggccatctcgctggctgggatggagtgggagtccaaggcccaggtgctattctttcttgtcatcatggtctcctttgccaactacctg
gtgggcacactgatcccagcatctgaagacaaagcctctaaaggcttctacagctaccatggggacatttttgttcagaacctggtacccgactggagag
gcattgatggtagcttctttggcatgttctccatcttcttcccttcggccactggcattctggcaggggccaacatctccggggacctcaaggatcctgc
tgtagccatccccaagggaacactcatggccattttctggaccaccatctcctaccttgccatctcagctaccatcggctcctgtgtggtgagagatgcc
tctggggatgtgaacgacaccatgactcctggtccaggcccctgcgaagggctagcctgtggctatggctggaacttcacagaatgctcccagcagcgca
gttgccgctatggcctcatcaactactaccagaccatgagcatggtgtctgccttcgcacccctgatcacagccggcatctttggcgccaccctgtcttc
tgccttggcctgtcttgtctcggctgctaaggtcttccagtgcctgtgtgaagaccagctgtaccctctgattggcttttttggcaaaggctatggcaag
aacagggagcctgtgcgtggctacctgctggcctatgccattgctgtggccttcatcatcattgctgaacttaacaccatcgcccccatcatttccaact
tcttcctctgctcctacgccctcatcaatttcagctgcttccatgcctccatcaccaactcacctggatggagaccctccttccgatactacagcaagtg
ggcggcgctgtttggggcagtgatctcggtggtcatcatgttcctgcttacctggtgggctgctctcattgccatcggagtcgtcctcttcctcctgctc
tatgtgatctacaagaagccagaggtgaactggggttcctcagtacaggctggctcctacaatctggccctgagctactccgtgggcctcaacgaggtgg
aagaccacatcaagaactaccgcccccagtgcctggtgcttacagggccccccaacttccgcccagcccttgtggactttgtgagcacatttacccagaa
cctaagcctaatgatttgtggtcatgtgctcattggacctggcaagcagagagtgcccgagctccggctcatcgccagtggacacaccaagtggctgaac
aagaggaagatcaaggccttctactctgacgtcatcgctgaggacttgcgcagtggcgtccagattctcatgcaggcctcagggcttgggagaatgaagc
cgaacattctggtggttggattcaagaggaactggcagtctgcccacccagccacagtggaggactacattggtgtcctgcatgatgcctttgacttcaa
ctacggggtttgtgtcatgaggatgcgcgaggggctcaatgtctccgaggctctgcagacccacacaaccccagaggcattgatacaagaggagcaggct
agcaccatcttccagtcggagcaaggcaagaagaccattgacatctactggctgttcgatgatggaggtctcaccctcctcatcccctatctcctgcacc
gcaagaagagatggggcaaatgcaagatccgagtgtttgttgggggccagatcaacaggatggacgaagagagaaaggcgatcatctctctgttgagcaa
gttcagactgggattccatgaagttcacgtccttcccgacatcaaccagaagccccaggccgagcacaccaagcggtttgaggacatgattgcacccttc
cgcctcaatgatggcttcaaggacgaggccaccgtcactgagatgaggagggactgtccctggaagatctcagatgaagagattaacaagaacagaatta
agtcccttcggcaggtgagactgagtgagatactgctggattactctcgggatgctgctctgatcatccttaccctgcccattggaaggaaggggaagtg
ccccagttcgctgtacatggcctggctggagaccctgtcccaggacctcagacctcccgtcctcctcatccgaggcaaccaggagaatgtgctcaccttt
tactgccagtgagcggccgc

Amino Acid Sequence

MDYKDDDDKGSPEFMAELPVTELPGDALCSGRFTISTLMGGDEPPPAACDSSQPSHLTHGSTLYMRTFGYNTIDVVPAYEHYANSALPGEPRKVRPTLAD
LHSFLKEGSHLHALAFDGRQGRELTDGLVEDETGTNSEKSPGEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWLIILLSVMVTSITGLSI
SAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGTPIVDPINDIRIIGVVTVTVLLAISLAGMEWESKAQVLF
FLVIMVSFANYLVGTLIPASEDKASKGFYSYHGDIFVQNLVPDWRGIDGSFFGMFSIFFPSATGILAGANISGDLKDPAVAIPKGTLMAIFWTTISYLAI
SATIGSCVVRDASGDVNDTMTPGPGPCEGLACGYGWNFTECSQQRSCRYGLINYYQTMSMVSAFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLY
PLIGFFGKGYGKNREPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFRYYSKWAALFGAVISVVIMFLLTWWAA
LIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVSTFTQNLSLMICGHVLIGPGKQRVPEL
RLIASGHTKWLNKRKIKAFYSDVIAEDLRSGVQILMQASGLGRMKPNILVVGFKRNWQSAHPATVEDYIGVLHDAFDFNYGVCVMRMREGLNVSEALQTH
TTPEALIQEEQASTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLHRKKRWGKCKIRVFVGGQINRMDEERKAIISLLSKFRLGFHEVHVLPDINQKPQAE
HTKRFEDMIAPFRLNDGFKDEATVTEMRRDCPWKISDEEINKNRIKSLRQVRLSEILLDYSRDAALIILTLPIGRKGKCPSSLYMAWLETLSQDLRPPVL
LIRGNQENVLTFYCQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU14373
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: plasmid not full sequenced internally