cDNA Clone NCC

• Plasmid: pSG5-SLC12A3 (hNCC) R135A
• Parent Plasmid: pSG5
• Expressed: SLC12A3 (hNCC) R135A
• Genbank: P55017
• Species: Human

Sequence of Insert

GAATTCATGGCAGAACTGCCCACAACAGAGACGCCTGGGGACGCCACTTT
GTGCAGCGGGCGCTTCACCATCAGCACACTGCTGAGCAGTGATGAGCCCT
CTCCACCAGCTGCCTATGACAGCAGCCACCCCAGCCACCTGACCCACAGC
AGCACCTTCTGCATGCGCACCTTTGGCTACAACACGATCGATGTGGTGCC
CACATATGAGCACTATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGG
TCCGGCCCACACTGGCTGACCTGCACTCCTTCCTCAAGCAGGAAGGCAGA
CACCTGCATGCCCTGGCCTTTGACAGCCGGCCCAGCCACGAGATGACTGA
TGGGCTGGTGGAGGGCGAGGCAGGCACCAGCAGCGAGAAGAACCCCGAGG
AGCCAGTGGCCTTCGGCTGGGTCAAGGGGGTGATGATTCGTTGCATGCTC
AACATTTGGGGCGTGATCCTCTACCTGCGGCTGCCCTGGATTACGGCCCA
GGCAGGCATCGTCCTGACCTGGATCATCATCCTGCTGTCGGTCACGGTGA
CCTCCATCACAGGCCTCTCCATCTCAGCCATCTCCACCAATGGCAAGGTC
AAGTCAGGTGGCACCTACTTCCTCATCTCCCGGAGTCTGGGCCCAGAGCT
TGGGGGCTCCATCGGCCTCATTTTCGCTTTCGCCAATGCCGTGGGTGTGG
CCATGCACACGGTGGGCTTTGCAGAGACCGTGCGGGACCTGCTCCAGGAG
TATGGGGCACCCATCGTGGACCCCATTAACGACATCCGCATCATTGGCGT
GGTCTCGGTCACTGTGCTGCTGGCCATCTCCCTGGCTGGCATGGAGTGGG
AGTCCAAGGCCCAGGTGCTGTTCTTCCTTGTCATCATGGTCTCCTTTGCC
AACTATTTAGTGGGGACGCTGATCCCCCCATCTGAGGACAAGGCCTCCAA
AGGCTTCTTCAGCTACCGGGCGGACATTTTTGTCCAAAACTTGGTGCCTG
ACTGGCGGGGTCCAGATGGCACCTTCTTCGGAATGTTCTCCATCTTCTTC
CCCTCGGCCACAGGCATCCTGGCAGGGGCCAACATATCTGGTGACCTCAA
GGACCCTGCTATAGCCATCCCCAAGGGGACCCTCATGGCCATTTTCTGGA
CGACCATTTCCTACCTGGCCATCTCAGCCACCATTGGCTCCTGCGTGGTG
CGTGATGCCTCTGGGGTCCTGAATGACACAGTGACCCCTGGCTGGGGTGC
CTGCGAGGGGCTGGCCTGCAGCTATGGCTGGAACTTCACCGAGTGCACCC
AGCAGCACAGCTGCCACTACGGCCTCATCAACTATTACCAGACCATGAGC
ATGGTGTCAGGCTTCGCGCCCCTGATCACGGCTGGCATCTTCGGGGCCAC
CCTCTCCTCTGCCCTGGCCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGT
GCCTTTGCGAGGACCAGCTGTACCCACTGATCGGCTTCTTCGGCAAAGGC
TATGGCAAGAACAAGGAGCCCGTGCGTGGCTACCTGCTGGCCTACGCCAT
CGCTGTGGCCTTCATCATCATCGCTGAGCTCAACACCATAGCCCCCATCA
TTTCCAACTTCTTCCTCTGCTCCTATGCCCTCATCAACTTCAGCTGCTTC
CACGCCTCCATCACCAACTCGCCTGGGTGGAGACCTTCATTCCAATACTA
CAACAAGTGGGCGGCGCTGTTTGGGGCTATCATCTCCGTGGTCATCATGT
TCCTCCTCACCTGGTGGGCGGCCCTCATCGCCATTGGCGTGGTGCTCTTC
CTCCTGCTCTATGTCATCTACAAGAAGCCAGAGGTAAATTGGGGCTCCTC
GGTACAGGCTGGCTCCTACAACCTGGCCCTCAGCTACTCGGTGGGCCTCA
ATGAGGTGGAAGACCACATCAAGAACTACCGCCCCCAGTGCCTGGTGCTC
ACGGGGCCCCCCAACTTCCGCCCGGCCCTGGTGGACTTTGTGGGCACCTT
CACCCGGAACCTCAGCCTGATGATCTGTGGCCACGTGCTCATCGGACCCC
ACAAGCAGAGGATGCCTGAGCTCCAGCTCATCGCCAACGGGCACACCAAG
TGGCTGAACAAGAGGAAGATCAAGGCCTTCTACTCGGATGTCATTGCTGA
GGACCTCCGCAGAGGCGTCCAGATCCTCATGCAGGCCGCAGGTCTCGGGA
GAATGAAGCCCAACATTCTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCG
GCTCACCCGGCCACAGTGGAAGACTACATTGGCATCCTCCATGATGCCTT
TGATTTCAACTATGGCGTGTGTGTCATGAGGATGCGGGAGGGACTCAACG
TGTCCAAGATGATGCAGGCGCACATTAACCCCGTGTTTGACCCAGCGGAG
GACGGGAAGGAAGCCAGCGCCAGAGTGGACCCCAAGGCCCTGGTGAAGGA
GGAGCAGGCCACCACCATCTTCCAGTCGGAGCAGGGCAAGAAGACCATAG
ACATCTACTGGCTCTTTGACGATGGAGGCCTCACCCTCCTCATTCCCTAT
CTCCTTGGCCGCAAGAGGAGGTGGAGCAAATGCAAGATCCGTGTGTTCGT
AGGTGGCCAGATTAACAGGATGGACCAGGAGAGAAAGGCGATCATTTCTC
TGCTGAGCAAGTTCCGACTGGGATTCCATGAAGTCCACATCCTCCCTGAC
ATCAACCAGAACCCTCGGGCTGAGCACACCAAGAGGTTTGAGGACATGAT
TGCACCCTTCCGTCTGAATGATGGCTTCAAGGATGAGGCCACTGTCAACG
AGATGCGGCGGGACTGCCCCTGGAAGATCTCAGATGAGGAGATTACGAAG
AACAGAGTCAAGTCCCTTCGGCAGGTGAGGCTGAATGAGATTGTGCTGGA
TTACTCCCGAGACGCTGCTCTCATCGTCATCACTTTGCCCATAGGGAGGA
AGGGGAAGTGCCCCAGCTCGCTGTACATGGCCTGGCTGGAGACCCTGTCC
CAGGACCTCAGACCTCCAGTCATCCTGATCCGAGGAAACCAGGAAAACGT
GCTCACCTTTTACTGCCAGTAAGGATCC

Amino Acid Sequence

MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSST
FCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHL
HALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVAFGWVKGVMIRCMLNI
WGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKS
GGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYG
APIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANY
LVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPS
ATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRD
ASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMV
SGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYG
KNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHA
SITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLL
LYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTG
PPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWL
NKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAH
PATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDG
KEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLL
GRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDIN
QNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNR
VKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQD
LRPPVILIRGNQENVLTFYCQ*

• Vector Type: Other
• Antibiotic: Amp
• DU Number: DU4913
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with no tag.