cDNA Clone GYS2

• Plasmid: pEBG6P-1 GYS2
• Parent Plasmid: pEBG6P
• Expressed: GST-GYS2
• Genbank: BC126310
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGCTTCGAGGCCGATCCCTCTCTGTAACATCCCTGGGT
GGGCTTCCCCAGTGGGAAGTCGAAGAACTTCCTGTGGAGGAGTTACTGCT
CTTTGAAGTTGCTTGGGAAGTGACCAATAAAGTTGGAGGCATCTATACTG
TGATTCAGACAAAGGCCAAAACAACAGCAGATGAATGGGGAGAGAACTAT
TTTCTGATAGGTCCATATTTTGAGCATAATATGAAGACTCAGGTGGAACA
GTGTGAACCTGTAAATGATGCTGTCAGAAGAGCAGTGGACGCAATGAATA
AGCATGGCTGCCAGGTGCATTTTGGAAGATGGCTGATAGAAGGAAGTCCT
TATGTGGTACTTTTTGACATAGGCTATTCAGCTTGGAATCTGGACAGGTG
GAAGGGTGACCTCTGGGAAGCATGCAGTGTCGGCATTCCTTATCATGACC
GAGAAGCCAATGATATGCTGATATTTGGATCTTTAACTGCCTGGTTCTTA
AAAGAGGTGACAGATCATGCAGATGGTAAATATGTCGTTGCCCAATTCCA
TGAATGGCAGGCTGGAATTGGACTGATCCTTTCTCGAGCCAGGAAACTTC
CTATTGCCACAATATTTACAACCCACGCTACACTACTTGGGAGGTATCTC
TGTGCAGCAAATATTGATTTCTACAACCATCTTGATAAGTTTAACATTGA
CAAAGAGGCTGGGGAAAGGCAGATTTACCACCGGTACTGCATGGAGCGAG
CTTCCGTTCATTGCGCTCACGTGTTCACCACGGTTTCTGAAATAACAGCA
ATAGAAGCTGAACATATGCTGAAGAGAAAGCCTGATGTAGTTACTCCAAA
CGGCTTGAATGTTAAGAAATTTTCAGCAGTGCATGAGTTTCAAAATCTAC
ATGCCATGTACAAGGCCAGAATCCAAGATTTTGTTCGAGGTCATTTCTAT
GGTCATCTCGACTTTGATCTTGAAAAGACTTTGTTCCTTTTCATTGCTGG
GAGGTATGAGTTTTCAAACAAAGGAGCTGACATCTTCCTAGAATCCTTAT
CCAGGCTAAATTTCCTGCTGAGGATGCATAAAAGTGACATCACAGTGGTG
GTGTTTTTCATTATGCCTGCCAAGACAAATAATTTCAACGTGGAAACCCT
GAAAGGACAAGCAGTGCGAAAACAGCTGTGGGATGTTGCACATTCTGTGA
AGGAAAAGTTTGGAAAAAAACTCTATGATGCATTATTAAGAGGAGAAATT
CCTGACCTGAACGATATTTTAGATCGAGATGATCTAACAATTATGAAAAG
AGCCATCTTTTCAACTCAGCGACAGTCATTGCCCCCAGTGACCACGCACA
ACATGATTGATGACTCCACCGACCCCATCCTCAGCACCATTAGACGGATT
GGACTTTTCAACAACCGCACAGATAGAGTCAAGGTGATTTTGCACCCAGA
GTTTCTATCCTCCACCAGTCCCTTACTACCCATGGACTATGAAGAGTTTG
TTAGAGGTTGTCATCTTGGAGTATTTCCATCATACTATGAACCCTGGGGT
TATACTCCAGCTGAATGCACTGTGATGGGTATCCCCAGTGTGACCACGAA
TCTCTCCGGGTTTGGCTGTTTCATGCAGGAGCACGTGGCTGATCCTACTG
CTTACGGTATTTACATCGTTGACAGGCGGTTCCGTTCTCCAGATGATTCT
TGCAATCAGCTGACTAAGTTTCTCTATGGATTTTGCAAACAGTCACGCCG
CCAAAGGATTATCCAGAGGAACAGAACTGAGAGGCTCTCAGATCTTCTGG
ATTGGAGATACTTAGGCAGATATTACCAGCATGCCAGACACCTGACATTA
AGCAGAGCTTTTCCAGATAAATTCCATGTGGAACTAACATCACCACCAAC
GACAGAAGGATTTAAATATCCCAGGCCTTCCTCAGTACCACCTTCTCCTT
CAGGGTCTCAGGCCTCCAGTCCTCAGAGCAGTGATGTGGAAGATGAAGTG
GAGGATGAGAGATACGATGAGGAAGAGGAGGCTGAAAGGGATCGGTTAAA
TATCAAGTCACCATTTTCACTGAGCCACGTTCCTCATGGGAAGAAAAAGC
TGCATGGTGAATATAAGAACTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMLRGRSLSVTSLG
GLPQWEVEELPVEELLLFEVAWEVTNKVGGIYTVIQTKAKTTADEWGENY
FLIGPYFEHNMKTQVEQCEPVNDAVRRAVDAMNKHGCQVHFGRWLIEGSP
YVVLFDIGYSAWNLDRWKGDLWEACSVGIPYHDREANDMLIFGSLTAWFL
KEVTDHADGKYVVAQFHEWQAGIGLILSRARKLPIATIFTTHATLLGRYL
CAANIDFYNHLDKFNIDKEAGERQIYHRYCMERASVHCAHVFTTVSEITA
IEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQDFVRGHFY
GHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSDITVV
VFFIMPAKTNNFNVETLKGQAVRKQLWDVAHSVKEKFGKKLYDALLRGEI
PDLNDILDRDDLTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILSTIRRI
GLFNNRTDRVKVILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYYEPWG
YTPAECTVMGIPSVTTNLSGFGCFMQEHVADPTAYGIYIVDRRFRSPDDS
CNQLTKFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHARHLTL
SRAFPDKFHVELTSPPTTEGFKYPRPSSVPPSPSGSQASSPQSSDVEDEV
EDERYDEEEEAERDRLNIKSPFSLSHVPHGKKKLHGEYKN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4880
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST tag.