cDNA Clone IRS2

• Plasmid: pCMV5 IRS2
• Parent Plasmid: pCMV5
• Expressed: IRS2
• Genbank: XP_357863
• Species: Mouse

Sequence of Insert

GGATCCATGGCTAGCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGG
GGACGGCCCGAACCTCAATAACAACAACAACAACAACAACCACAGCGTGC
GCAAGTGCGGCTACCTGCGCAAGCAGAAGCACGGCCACAAGCGCTTTTTC
GTGTTGCGCGGCCCCGGCACGGGCGGCGACGAGGCATCCGCGGCTGGGGG
GTCGCCGCCGCAGCCTCCGCGGCTGGAGTACTACGAGAGCGAGAAGAAGT
GGAGGAGCAAGGCGGGCGCGCCGAAGCGAGTGATCGCGCTCGACTGCTGT
CTGAACATCAACAAGCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCT
CTACACCAAGGACGAGTACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGC
AGGAGGGCTGGTACCGCGCACTCACCGACTTGGTCAGCGAAGGCCGCTCT
GGCGAGGGGGGCTCGGGCACCACCGGAGGCTCTTGCAGCGCCTCTCTCCC
GGGCGTCCTGGGCGGCTCAGCGGGCGCCGCTGGCTGCGATGACAACTACG
GGCTCGTGACACCCGCCACGGCCGTCTACCGCGAGGTGTGGCAGGTGAAC
CTGAAACCTAAGGGACTGGGCCAGAGCAAGAACCTGACTGGTGTATACCG
CCTATGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAAC
AGCCGTCGGTGACGCTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCG
GACAGCTTCTTCTTCATCGAGGTGGGCCGTTCGGCCGTCACCGGTCCCGG
GGAGCTGTGGATGCAAGCCGACGACTCGGTGGTGGCGCAGAACATCCATG
AGACCATCCTAGAAGCTATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGG
CCTCGCAGCAAGAGTCAGTCGTCCGGGTCGTCAGCCACGCATCCCATCAG
CGTGCCGGGCGCGCGCCGCCACCACCACCTAGTCAACCTACCCCCTAGCC
AGACCGGCCTGGTGCGCCGCTCGCGCACTGACAGCCTGGCGGCCACCCCC
CCAGCAGCCAAGTGCACTTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGA
CGGCGGCGCGGCAGGCGGGGCCGGGACGGCAGGAGGCAGGCCGATGTCGG
TGGCAGGGAGCCCCCTGAGTCCCGGGCCGGTGCGCGCGCCCCTTAGCCGC
TCGCACACCCTGAGCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCT
GGCGCCGGCAGGGGGAGCCCTGCAACACAGCCGCTCCATGTCCATGCCCG
TGGCGCACTCACCTCCTGCAGCCACCAGCCCAGGCAGCCTGTCCTCCAGC
AGTGGGCACGGCTCGGGCTCCTACCCGCTGCCACCTGGCTCCCACCCGCA
CCTGCCTCATCCACTGCATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTA
GTGCCTCCGCCTCGGGCTCCCCCAGCGACCCGGGTTTCATGTCCCTTGAC
GAGTATGGCTCCAGCCCTGGCGACCTGAGAGCCTTCAGTAGCCACAGGAG
CAACACACCCGAGTCAATAGCGGAGACCCCGCCAGCCAGAGATGGCAGTG
GGGGCGAACTCTATGGGTACATGAGCATGGATAGACCCCTGAGCCACTGT
GGCCGCCCTTACCGTAGGGTCTCAGGGGATGGGGCCCAGGACCTGGATAG
AGGACTGAGGAAGAGGACTTATTCCCTAACCACGCCTGCCAGGCAGCGGC
AGGTACCTCAGCCTTCCTCTGCCTCTCTAGATGAATACACTCTCATGAGG
GCCACCTTCTCTGGTAGTTCAGGTCGCCTCTGCCCATCCTTCCCTGCGTC
CTCTCCCAAAGTGGCCTACAACCCTTACCCAGAGGACTATGGAGACATTG
AGATTGGTTCTCACAAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGC
TACATGCCCATGACCCCTGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAG
CTGCAAGAGCGATGACTACATGCCCATGAGCCCCACAAGCGTGTCTGCTC
CCAAGCAGATCCTGCAGCCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGA
GCAGCCGTGCCAGCACCCCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAA
CGGAGGTGGCTACAAAGCCAGCTCCCCAGCGGAGAGCTCCCCAGAAGACA
GTGGGTACATGCGAATGTGGTGTGGCTCCAAGCTGTCTATGGAGAACCCA
GACCCTAAGCTACTCCCCAACGGGGACTACCTCAACATGTCCCCCAGCGA
GGCAGGCACTGCAGGGACCCCACCTGACTTCTCAGCAGCTTTGCGTGGAG
GCAGTGAAGGCCTCAAAGGCATCCCGGGCCACTGCTACAGCTCTTTGCCC
CGCTCTTATAAGGCTCCCTGTTCCTGCAGCGGAGACAATGACCAGTATGT
GCTCATGAGCTCCCCTGTGGGCCGGATCTTGGAAGAGGAGAGACTGGAGC
CCCAGGCCACCCCAGGGGCTGGCACCTTTGGGGCAGCTGGTGGTAGTCAT
ACCCAGCCTCATCACTCAGCAGTGCCTTCCTCCATGAGGCCGAGTGCCAT
CGGTGGCCGCCCTGAGGGCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGC
CTACACGCCTATCGCTAGAGGGACTGCAGACCCTTCCCAGCATGCAAGAG
TACCCTCTACCCACAGAGCCCAAGAGCCCTGGCGAGTACATCAACATTGA
CTTTGGTGAGGCAGGTACCCGTCTGTCTCCGCCTGCCCCCCCACTACTGG
CATCCGCGGCCTCATCTTCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCA
TCCCTGGGTTCAGGAACCCCAGGCACCAGCAGCGACAGCCGGCAGCGCTC
TCCACTCTCTGACTATATGAACCTGGACTTCAGTTCTCCCAAGTCCCCCA
AGCCTAGCACCCGCAGTGGGGACACAGTAGGCTCCATGGATGGCCTTCTC
TCTCCAGAGGCTTCATCCCCATACCCACCACTGCCCCCACGTCCTTCCAC
TTCCCCTTCCTCCTTACAGCAGCCTCTGCCACCTGCCCCGGGAGACCTAT
ACCGCCTGCCTCCAGCATCAGCTGCCACTTCCCAGGGTCCCACTGCTGGC
TCCTCAATGTCCTCCGAGCCTGGGGATAATGGTGACTATACCGAGATGGC
CTTTGGTGTGGCTGCAACCCCGCCACAACCTATCGTGGCACCTCCAAAGC
CAGAAGGTGCCCGAGTGGCCAGTCCCACATCGGGCTTGAAGCGGCTAAGT
CTCATGGATCAGGTATCTGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCC
CCCTGACCCCCACCGGGGTGCTAAGGTCATCCGTGCAGACCCACAGGGGG
GACGTCGTCGCCACAGTTCAGAGACCTTTTCCTCTACCACCACCGTCACC
CCAGTGTCCCCATCCTTTGCCCACAATTCCAAGCGCCACAATTCGGCCTC
TGTGGAAAATGTCTCACTCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGG
GAGGAGGTGATGAGCCGCCCACATCCCCAGGACAGGCACAGCCCTTGGTG
GCTGTGCCCCCAGTGCCACAGGCTAGGCCGTGGAACCCCGGTCAGCCCGG
AGCTTTGATTGGCTGTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGA
CCTCCGTGGGTTTCCAGAACGGCCTCAACTATATCGCCATCGATGTGAGA
GGCGAGCAGGGGTCCTTGGCGCAGTCTCAGCCGCAGCCAGGAGACAAGAA
CTCCTGGAGCCGGACCCGTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAG
GCTCTGGCGCCAGCGGAGTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCC
TCTGCCAGCACCTATGCAAGCATCGACTTCCTGTCCCATCACTTGAAGGA
AGCCACAGTCGTGAAAGAGTGAGCGGCCGC

Amino Acid Sequence

MASAPLPGPPASAGGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVL
RGPGTGGDEASAAGGSPPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLN
INKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRSGE
GGSGTTGGSCSASLPGVLGGSAGAAGCDDNYGLVTPATAVYREVWQVNLK
PKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDS
FFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPR
SKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPA
AKCTSCRVRTASEGDGGAAGGAGTAGGRPMSVAGSPLSPGPVRAPLSRSH
TLSAGCGGRPSKVTLAPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSG
HGSGSYPLPPGSHPHLPHPLHHPQGQRPSSGSASASGSPSDPGFMSLDEY
GSSPGDLRAFSSHRSNTPESIAETPPARDGSGGELYGYMSMDRPLSHCGR
PYRRVSGDGAQDLDRGLRKRTYSLTTPARQRQVPQPSSASLDEYTLMRAT
FSGSSGRLCPSFPASSPKVAYNPYPEDYGDIEIGSHKSSSSNLGADDGYM
PMTPGAALRSGGPNSCKSDDYMPMSPTSVSAPKQILQPRLAAALPPSGAA
VPAPPSGVGRTFPVNGGGYKASSPAESSPEDSGYMRMWCGSKLSMENPDP
KLLPNGDYLNMSPSEAGTAGTPPDFSAALRGGSEGLKGIPGHCYSSLPRS
YKAPCSCSGDNDQYVLMSSPVGRILEEERLEPQATPGAGTFGAAGGSHTQ
PHHSAVPSSMRPSAIGGRPEGFLGQRCRAVRPTRLSLEGLQTLPSMQEYP
LPTEPKSPGEYINIDFGEAGTRLSPPAPPLLASAASSSSLLSASSPASSL
GSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPSTRSGDTVGSMDGLLSP
EASSPYPPLPPRPSTSPSSLQQPLPPAPGDLYRLPPASAATSQGPTAGSS
MSSEPGDNGDYTEMAFGVAATPPQPIVAPPKPEGARVASPTSGLKRLSLM
DQVSGVEAFLQVSQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPV
SPSFAHNSKRHNSASVENVSLRKSSEGSSTLGGGDEPPTSPGQAQPLVAV
PPVPQARPWNPGQPGALIGCPGGSSSPMRRETSVGFQNGLNYIAIDVRGE
QGSLAQSQPQPGDKNSWSRTRSLGGLLGTVGGSGASGVCGGPGTGALPSA
STYASIDFLSHHLKEATVVKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4871
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with no tag. Note that kozak sequence has been included in this construct.