cDNA Clone IRS2

• Plasmid: pEBG6P-1 IRS2
• Parent Plasmid: pEBG6P
• Expressed: GST-IRS2
• Genbank: XP_357863
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGGCTAGCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCG
GGCGGGGACGGCCCGAACCTCAATAACAACAACAACAACAACAACCACAG
CGTGCGCAAGTGCGGCTACCTGCGCAAGCAGAAGCACGGCCACAAGCGCT
TTTTCGTGTTGCGCGGCCCCGGCACGGGCGGCGACGAGGCATCCGCGGCT
GGGGGGTCGCCGCCGCAGCCTCCGCGGCTGGAGTACTACGAGAGCGAGAA
GAAGTGGAGGAGCAAGGCGGGCGCGCCGAAGCGAGTGATCGCGCTCGACT
GCTGTCTGAACATCAACAAGCGCGCGGACGCCAAGCACAAGTACCTGATC
GCCCTCTACACCAAGGACGAGTACTTCGCTGTAGCGGCGGAGAACGAGCA
GGAGCAGGAGGGCTGGTACCGCGCACTCACCGACTTGGTCAGCGAAGGCC
GCTCTGGCGAGGGGGGCTCGGGCACCACCGGAGGCTCTTGCAGCGCCTCT
CTCCCGGGCGTCCTGGGCGGCTCAGCGGGCGCCGCTGGCTGCGATGACAA
CTACGGGCTCGTGACACCCGCCACGGCCGTCTACCGCGAGGTGTGGCAGG
TGAACCTGAAACCTAAGGGACTGGGCCAGAGCAAGAACCTGACTGGTGTA
TACCGCCTATGCCTGTCTGCGCGCACCATCGGCTTCGTGAAGCTCAATTG
CGAACAGCCGTCGGTGACGCTGCAGCTTATGAACATTCGCCGCTGCGGCC
ACTCGGACAGCTTCTTCTTCATCGAGGTGGGCCGTTCGGCCGTCACCGGT
CCCGGGGAGCTGTGGATGCAAGCCGACGACTCGGTGGTGGCGCAGAACAT
CCATGAGACCATCCTAGAAGCTATGAAGGCACTCAAAGAGCTCTTCGAGT
TCCGGCCTCGCAGCAAGAGTCAGTCGTCCGGGTCGTCAGCCACGCATCCC
ATCAGCGTGCCGGGCGCGCGCCGCCACCACCACCTAGTCAACCTACCCCC
TAGCCAGACCGGCCTGGTGCGCCGCTCGCGCACTGACAGCCTGGCGGCCA
CCCCCCCAGCAGCCAAGTGCACTTCGTGCCGGGTTCGTACGGCCAGCGAG
GGCGACGGCGGCGCGGCAGGCGGGGCCGGGACGGCAGGAGGCAGGCCGAT
GTCGGTGGCAGGGAGCCCCCTGAGTCCCGGGCCGGTGCGCGCGCCCCTTA
GCCGCTCGCACACCCTGAGCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTG
ACTCTGGCGCCGGCAGGGGGAGCCCTGCAACACAGCCGCTCCATGTCCAT
GCCCGTGGCGCACTCACCTCCTGCAGCCACCAGCCCAGGCAGCCTGTCCT
CCAGCAGTGGGCACGGCTCGGGCTCCTACCCGCTGCCACCTGGCTCCCAC
CCGCACCTGCCTCATCCACTGCATCACCCCCAAGGCCAGCGTCCGTCCAG
CGGTAGTGCCTCCGCCTCGGGCTCCCCCAGCGACCCGGGTTTCATGTCCC
TTGACGAGTATGGCTCCAGCCCTGGCGACCTGAGAGCCTTCAGTAGCCAC
AGGAGCAACACACCCGAGTCAATAGCGGAGACCCCGCCAGCCAGAGATGG
CAGTGGGGGCGAACTCTATGGGTACATGAGCATGGATAGACCCCTGAGCC
ACTGTGGCCGCCCTTACCGTAGGGTCTCAGGGGATGGGGCCCAGGACCTG
GATAGAGGACTGAGGAAGAGGACTTATTCCCTAACCACGCCTGCCAGGCA
GCGGCAGGTACCTCAGCCTTCCTCTGCCTCTCTAGATGAATACACTCTCA
TGAGGGCCACCTTCTCTGGTAGTTCAGGTCGCCTCTGCCCATCCTTCCCT
GCGTCCTCTCCCAAAGTGGCCTACAACCCTTACCCAGAGGACTATGGAGA
CATTGAGATTGGTTCTCACAAGAGTTCCAGCAGTAACCTGGGGGCAGATG
ATGGCTACATGCCCATGACCCCTGGGGCAGCCCTTAGGAGTGGTGGTCCC
AATAGCTGCAAGAGCGATGACTACATGCCCATGAGCCCCACAAGCGTGTC
TGCTCCCAAGCAGATCCTGCAGCCACGCTTGGCAGCGGCCTTGCCCCCTT
CCGGAGCAGCCGTGCCAGCACCCCCTTCAGGGGTGGGCAGGACCTTCCCA
GTAAACGGAGGTGGCTACAAAGCCAGCTCCCCAGCGGAGAGCTCCCCAGA
AGACAGTGGGTACATGCGAATGTGGTGTGGCTCCAAGCTGTCTATGGAGA
ACCCAGACCCTAAGCTACTCCCCAACGGGGACTACCTCAACATGTCCCCC
AGCGAGGCAGGCACTGCAGGGACCCCACCTGACTTCTCAGCAGCTTTGCG
TGGAGGCAGTGAAGGCCTCAAAGGCATCCCGGGCCACTGCTACAGCTCTT
TGCCCCGCTCTTATAAGGCTCCCTGTTCCTGCAGCGGAGACAATGACCAG
TATGTGCTCATGAGCTCCCCTGTGGGCCGGATCTTGGAAGAGGAGAGACT
GGAGCCCCAGGCCACCCCAGGGGCTGGCACCTTTGGGGCAGCTGGTGGTA
GTCATACCCAGCCTCATCACTCAGCAGTGCCTTCCTCCATGAGGCCGAGT
GCCATCGGTGGCCGCCCTGAGGGCTTCCTGGGCCAGCGATGTCGGGCAGT
GCGGCCTACACGCCTATCGCTAGAGGGACTGCAGACCCTTCCCAGCATGC
AAGAGTACCCTCTACCCACAGAGCCCAAGAGCCCTGGCGAGTACATCAAC
ATTGACTTTGGTGAGGCAGGTACCCGTCTGTCTCCGCCTGCCCCCCCACT
ACTGGCATCCGCGGCCTCATCTTCTTCACTGCTCTCAGCTAGTAGTCCTG
CTTCATCCCTGGGTTCAGGAACCCCAGGCACCAGCAGCGACAGCCGGCAG
CGCTCTCCACTCTCTGACTATATGAACCTGGACTTCAGTTCTCCCAAGTC
CCCCAAGCCTAGCACCCGCAGTGGGGACACAGTAGGCTCCATGGATGGCC
TTCTCTCTCCAGAGGCTTCATCCCCATACCCACCACTGCCCCCACGTCCT
TCCACTTCCCCTTCCTCCTTACAGCAGCCTCTGCCACCTGCCCCGGGAGA
CCTATACCGCCTGCCTCCAGCATCAGCTGCCACTTCCCAGGGTCCCACTG
CTGGCTCCTCAATGTCCTCCGAGCCTGGGGATAATGGTGACTATACCGAG
ATGGCCTTTGGTGTGGCTGCAACCCCGCCACAACCTATCGTGGCACCTCC
AAAGCCAGAAGGTGCCCGAGTGGCCAGTCCCACATCGGGCTTGAAGCGGC
TAAGTCTCATGGATCAGGTATCTGGGGTGGAGGCTTTCCTTCAAGTCAGC
CAGCCCCCTGACCCCCACCGGGGTGCTAAGGTCATCCGTGCAGACCCACA
GGGGGGACGTCGTCGCCACAGTTCAGAGACCTTTTCCTCTACCACCACCG
TCACCCCAGTGTCCCCATCCTTTGCCCACAATTCCAAGCGCCACAATTCG
GCCTCTGTGGAAAATGTCTCACTCAGGAAAAGCAGTGAAGGCAGCAGTAC
CCTGGGAGGAGGTGATGAGCCGCCCACATCCCCAGGACAGGCACAGCCCT
TGGTGGCTGTGCCCCCAGTGCCACAGGCTAGGCCGTGGAACCCCGGTCAG
CCCGGAGCTTTGATTGGCTGTCCTGGAGGCAGCAGTTCTCCCATGCGCAG
AGAGACCTCCGTGGGTTTCCAGAACGGCCTCAACTATATCGCCATCGATG
TGAGAGGCGAGCAGGGGTCCTTGGCGCAGTCTCAGCCGCAGCCAGGAGAC
AAGAACTCCTGGAGCCGGACCCGTAGCCTTGGGGGGCTCCTCGGCACCGT
CGGAGGCTCTGGCGCCAGCGGAGTGTGTGGGGGTCCAGGCACTGGAGCTT
TGCCCTCTGCCAGCACCTATGCAAGCATCGACTTCCTGTCCCATCACTTG
AAGGAAGCCACAGTCGTGAAAGAGTGAGCGGCCGC

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMASAPLPGPPASA
GGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGTGGDEASAA
GGSPPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLI
ALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRSGEGGSGTTGGSCSAS
LPGVLGGSAGAAGCDDNYGLVTPATAVYREVWQVNLKPKGLGQSKNLTGV
YRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTG
PGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHP
ISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCTSCRVRTASE
GDGGAAGGAGTAGGRPMSVAGSPLSPGPVRAPLSRSHTLSAGCGGRPSKV
TLAPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPLPPGSH
PHLPHPLHHPQGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFSSH
RSNTPESIAETPPARDGSGGELYGYMSMDRPLSHCGRPYRRVSGDGAQDL
DRGLRKRTYSLTTPARQRQVPQPSSASLDEYTLMRATFSGSSGRLCPSFP
ASSPKVAYNPYPEDYGDIEIGSHKSSSSNLGADDGYMPMTPGAALRSGGP
NSCKSDDYMPMSPTSVSAPKQILQPRLAAALPPSGAAVPAPPSGVGRTFP
VNGGGYKASSPAESSPEDSGYMRMWCGSKLSMENPDPKLLPNGDYLNMSP
SEAGTAGTPPDFSAALRGGSEGLKGIPGHCYSSLPRSYKAPCSCSGDNDQ
YVLMSSPVGRILEEERLEPQATPGAGTFGAAGGSHTQPHHSAVPSSMRPS
AIGGRPEGFLGQRCRAVRPTRLSLEGLQTLPSMQEYPLPTEPKSPGEYIN
IDFGEAGTRLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQ
RSPLSDYMNLDFSSPKSPKPSTRSGDTVGSMDGLLSPEASSPYPPLPPRP
STSPSSLQQPLPPAPGDLYRLPPASAATSQGPTAGSSMSSEPGDNGDYTE
MAFGVAATPPQPIVAPPKPEGARVASPTSGLKRLSLMDQVSGVEAFLQVS
QPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNSKRHNS
ASVENVSLRKSSEGSSTLGGGDEPPTSPGQAQPLVAVPPVPQARPWNPGQ
PGALIGCPGGSSSPMRRETSVGFQNGLNYIAIDVRGEQGSLAQSQPQPGD
KNSWSRTRSLGGLLGTVGGSGASGVCGGPGTGALPSASTYASIDFLSHHL
KEATVVKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4861
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.