cDNA Clone IRS2

• Plasmid: pGEX6P-1-IRS2
• Parent Plasmid: pGEX6P1
• Expressed: GST-IRS2
• Genbank: XP_357863
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGGCTA
GCGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGGGGACGGCCCGAAC
CTCAATAACAACAACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTA
CCTGCGCAAGCAGAAGCACGGCCACAAGCGCTTTTTCGTGTTGCGCGGCC
CCGGCACGGGCGGCGACGAGGCATCCGCGGCTGGGGGGTCGCCGCCGCAG
CCTCCGCGGCTGGAGTACTACGAGAGCGAGAAGAAGTGGAGGAGCAAGGC
GGGCGCGCCGAAGCGAGTGATCGCGCTCGACTGCTGTCTGAACATCAACA
AGCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCTCTACACCAAGGAC
GAGTACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGCAGGAGGGCTGGTA
CCGCGCACTCACCGACTTGGTCAGCGAAGGCCGCTCTGGCGAGGGGGGCT
CGGGCACCACCGGAGGCTCTTGCAGCGCCTCTCTCCCGGGCGTCCTGGGC
GGCTCAGCGGGCGCCGCTGGCTGCGATGACAACTACGGGCTCGTGACACC
CGCCACGGCCGTCTACCGCGAGGTGTGGCAGGTGAACCTGAAACCTAAGG
GACTGGGCCAGAGCAAGAACCTGACTGGTGTATACCGCCTATGCCTGTCT
GCGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAACAGCCGTCGGTGAC
GCTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCGGACAGCTTCTTCT
TCATCGAGGTGGGCCGTTCGGCCGTCACCGGTCCCGGGGAGCTGTGGATG
CAAGCCGACGACTCGGTGGTGGCGCAGAACATCCATGAGACCATCCTAGA
AGCTATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGGCCTCGCAGCAAGA
GTCAGTCGTCCGGGTCGTCAGCCACGCATCCCATCAGCGTGCCGGGCGCG
CGCCGCCACCACCACCTAGTCAACCTACCCCCTAGCCAGACCGGCCTGGT
GCGCCGCTCGCGCACTGACAGCCTGGCGGCCACCCCCCCAGCAGCCAAGT
GCACTTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGACGGCGGCGCGGCA
GGCGGGGCCGGGACGGCAGGAGGCAGGCCGATGTCGGTGGCAGGGAGCCC
CCTGAGTCCCGGGCCGGTGCGCGCGCCCCTTAGCCGCTCGCACACCCTGA
GCGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCTGGCGCCGGCAGGG
GGAGCCCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCACTCACC
TCCTGCAGCCACCAGCCCAGGCAGCCTGTCCTCCAGCAGTGGGCACGGCT
CGGGCTCCTACCCGCTGCCACCTGGCTCCCACCCGCACCTGCCTCATCCA
CTGCATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTAGTGCCTCCGCCTC
GGGCTCCCCCAGCGACCCGGGTTTCATGTCCCTTGACGAGTATGGCTCCA
GCCCTGGCGACCTGAGAGCCTTCAGTAGCCACAGGAGCAACACACCCGAG
TCAATAGCGGAGACCCCGCCAGCCAGAGATGGCAGTGGGGGCGAACTCTA
TGGGTACATGAGCATGGATAGACCCCTGAGCCACTGTGGCCGCCCTTACC
GTAGGGTCTCAGGGGATGGGGCCCAGGACCTGGATAGAGGACTGAGGAAG
AGGACTTATTCCCTAACCACGCCTGCCAGGCAGCGGCAGGTACCTCAGCC
TTCCTCTGCCTCTCTAGATGAATACACTCTCATGAGGGCCACCTTCTCTG
GTAGTTCAGGTCGCCTCTGCCCATCCTTCCCTGCGTCCTCTCCCAAAGTG
GCCTACAACCCTTACCCAGAGGACTATGGAGACATTGAGATTGGTTCTCA
CAAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGCTACATGCCCATGA
CCCCTGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAGCTGCAAGAGCGAT
GACTACATGCCCATGAGCCCCACAAGCGTGTCTGCTCCCAAGCAGATCCT
GCAGCCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGAGCAGCCGTGCCAG
CACCCCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAACGGAGGTGGCTAC
AAAGCCAGCTCCCCAGCGGAGAGCTCCCCAGAAGACAGTGGGTACATGCG
AATGTGGTGTGGCTCCAAGCTGTCTATGGAGAACCCAGACCCTAAGCTAC
TCCCCAACGGGGACTACCTCAACATGTCCCCCAGCGAGGCAGGCACTGCA
GGGACCCCACCTGACTTCTCAGCAGCTTTGCGTGGAGGCAGTGAAGGCCT
CAAAGGCATCCCGGGCCACTGCTACAGCTCTTTGCCCCGCTCTTATAAGG
CTCCCTGTTCCTGCAGCGGAGACAATGACCAGTATGTGCTCATGAGCTCC
CCTGTGGGCCGGATCTTGGAAGAGGAGAGACTGGAGCCCCAGGCCACCCC
AGGGGCTGGCACCTTTGGGGCAGCTGGTGGTAGTCATACCCAGCCTCATC
ACTCAGCAGTGCCTTCCTCCATGAGGCCGAGTGCCATCGGTGGCCGCCCT
GAGGGCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGCCTACACGCCTATC
GCTAGAGGGACTGCAGACCCTTCCCAGCATGCAAGAGTACCCTCTACCCA
CAGAGCCCAAGAGCCCTGGCGAGTACATCAACATTGACTTTGGTGAGGCA
GGTACCCGTCTGTCTCCGCCTGCCCCCCCACTACTGGCATCCGCGGCCTC
ATCTTCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCATCCCTGGGTTCAG
GAACCCCAGGCACCAGCAGCGACAGCCGGCAGCGCTCTCCACTCTCTGAC
TATATGAACCTGGACTTCAGTTCTCCCAAGTCCCCCAAGCCTAGCACCCG
CAGTGGGGACACAGTAGGCTCCATGGATGGCCTTCTCTCTCCAGAGGCTT
CATCCCCATACCCACCACTGCCCCCACGTCCTTCCACTTCCCCTTCCTCC
TTACAGCAGCCTCTGCCACCTGCCCCGGGAGACCTATACCGCCTGCCTCC
AGCATCAGCTGCCACTTCCCAGGGTCCCACTGCTGGCTCCTCAATGTCCT
CCGAGCCTGGGGATAATGGTGACTATACCGAGATGGCCTTTGGTGTGGCT
GCAACCCCGCCACAACCTATCGTGGCACCTCCAAAGCCAGAAGGTGCCCG
AGTGGCCAGTCCCACATCGGGCTTGAAGCGGCTAAGTCTCATGGATCAGG
TATCTGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCCCCCTGACCCCCAC
CGGGGTGCTAAGGTCATCCGTGCAGACCCACAGGGGGGACGTCGTCGCCA
CAGTTCAGAGACCTTTTCCTCTACCACCACCGTCACCCCAGTGTCCCCAT
CCTTTGCCCACAATTCCAAGCGCCACAATTCGGCCTCTGTGGAAAATGTC
TCACTCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGGGAGGAGGTGATGA
GCCGCCCACATCCCCAGGACAGGCACAGCCCTTGGTGGCTGTGCCCCCAG
TGCCACAGGCTAGGCCGTGGAACCCCGGTCAGCCCGGAGCTTTGATTGGC
TGTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGACCTCCGTGGGTTT
CCAGAACGGCCTCAACTATATCGCCATCGATGTGAGAGGCGAGCAGGGGT
CCTTGGCGCAGTCTCAGCCGCAGCCAGGAGACAAGAACTCCTGGAGCCGG
ACCCGTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAGGCTCTGGCGCCAG
CGGAGTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCCTCTGCCAGCACCT
ATGCAAGCATCGACTTCCTGTCCCATCACTTGAAGGAAGCCACAGTCGTG
AAAGAGTGAGCGGCCGCG

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMASAPLPGPPASAGGDGPN
LNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGTGGDEASAAGGSPPQ
PPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKD
EYFAVAAENEQEQEGWYRALTDLVSEGRSGEGGSGTTGGSCSASLPGVLG
GSAGAAGCDDNYGLVTPATAVYREVWQVNLKPKGLGQSKNLTGVYRLCLS
ARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWM
QADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGA
RRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCTSCRVRTASEGDGGAA
GGAGTAGGRPMSVAGSPLSPGPVRAPLSRSHTLSAGCGGRPSKVTLAPAG
GALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPLPPGSHPHLPHP
LHHPQGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFSSHRSNTPE
SIAETPPARDGSGGELYGYMSMDRPLSHCGRPYRRVSGDGAQDLDRGLRK
RTYSLTTPARQRQVPQPSSASLDEYTLMRATFSGSSGRLCPSFPASSPKV
AYNPYPEDYGDIEIGSHKSSSSNLGADDGYMPMTPGAALRSGGPNSCKSD
DYMPMSPTSVSAPKQILQPRLAAALPPSGAAVPAPPSGVGRTFPVNGGGY
KASSPAESSPEDSGYMRMWCGSKLSMENPDPKLLPNGDYLNMSPSEAGTA
GTPPDFSAALRGGSEGLKGIPGHCYSSLPRSYKAPCSCSGDNDQYVLMSS
PVGRILEEERLEPQATPGAGTFGAAGGSHTQPHHSAVPSSMRPSAIGGRP
EGFLGQRCRAVRPTRLSLEGLQTLPSMQEYPLPTEPKSPGEYINIDFGEA
GTRLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSD
YMNLDFSSPKSPKPSTRSGDTVGSMDGLLSPEASSPYPPLPPRPSTSPSS
LQQPLPPAPGDLYRLPPASAATSQGPTAGSSMSSEPGDNGDYTEMAFGVA
ATPPQPIVAPPKPEGARVASPTSGLKRLSLMDQVSGVEAFLQVSQPPDPH
RGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNSKRHNSASVENV
SLRKSSEGSSTLGGGDEPPTSPGQAQPLVAVPPVPQARPWNPGQPGALIG
CPGGSSSPMRRETSVGFQNGLNYIAIDVRGEQGSLAQSQPQPGDKNSWSR
TRSLGGLLGTVGGSGASGVCGGPGTGALPSASTYASIDFLSHHLKEATVV
KE*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4860
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.