cDNA Clone IRS2

• Plasmid: pCMV5-FLAG-1 IRS2
• Parent Plasmid: pCMV5
• Expressed: FLAG-IRS2
• Genbank: XP_357863
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGGCTAGCGC
GCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGGGGACGGCCCGAACCTCA
ATAACAACAACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTACCTG
CGCAAGCAGAAGCACGGCCACAAGCGCTTTTTCGTGTTGCGCGGCCCCGG
CACGGGCGGCGACGAGGCATCCGCGGCTGGGGGGTCGCCGCCGCAGCCTC
CGCGGCTGGAGTACTACGAGAGCGAGAAGAAGTGGAGGAGCAAGGCGGGC
GCGCCGAAGCGAGTGATCGCGCTCGACTGCTGTCTGAACATCAACAAGCG
CGCGGACGCCAAGCACAAGTACCTGATCGCCCTCTACACCAAGGACGAGT
ACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGCAGGAGGGCTGGTACCGC
GCACTCACCGACTTGGTCAGCGAAGGCCGCTCTGGCGAGGGGGGCTCGGG
CACCACCGGAGGCTCTTGCAGCGCCTCTCTCCCGGGCGTCCTGGGCGGCT
CAGCGGGCGCCGCTGGCTGCGATGACAACTACGGGCTCGTGACACCCGCC
ACGGCCGTCTACCGCGAGGTGTGGCAGGTGAACCTGAAACCTAAGGGACT
GGGCCAGAGCAAGAACCTGACTGGTGTATACCGCCTATGCCTGTCTGCGC
GCACCATCGGCTTCGTGAAGCTCAATTGCGAACAGCCGTCGGTGACGCTG
CAGCTTATGAACATTCGCCGCTGCGGCCACTCGGACAGCTTCTTCTTCAT
CGAGGTGGGCCGTTCGGCCGTCACCGGTCCCGGGGAGCTGTGGATGCAAG
CCGACGACTCGGTGGTGGCGCAGAACATCCATGAGACCATCCTAGAAGCT
ATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGGCCTCGCAGCAAGAGTCA
GTCGTCCGGGTCGTCAGCCACGCATCCCATCAGCGTGCCGGGCGCGCGCC
GCCACCACCACCTAGTCAACCTACCCCCTAGCCAGACCGGCCTGGTGCGC
CGCTCGCGCACTGACAGCCTGGCGGCCACCCCCCCAGCAGCCAAGTGCAC
TTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGACGGCGGCGCGGCAGGCG
GGGCCGGGACGGCAGGAGGCAGGCCGATGTCGGTGGCAGGGAGCCCCCTG
AGTCCCGGGCCGGTGCGCGCGCCCCTTAGCCGCTCGCACACCCTGAGCGC
CGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCTGGCGCCGGCAGGGGGAG
CCCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCACTCACCTCCT
GCAGCCACCAGCCCAGGCAGCCTGTCCTCCAGCAGTGGGCACGGCTCGGG
CTCCTACCCGCTGCCACCTGGCTCCCACCCGCACCTGCCTCATCCACTGC
ATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTAGTGCCTCCGCCTCGGGC
TCCCCCAGCGACCCGGGTTTCATGTCCCTTGACGAGTATGGCTCCAGCCC
TGGCGACCTGAGAGCCTTCAGTAGCCACAGGAGCAACACACCCGAGTCAA
TAGCGGAGACCCCGCCAGCCAGAGATGGCAGTGGGGGCGAACTCTATGGG
TACATGAGCATGGATAGACCCCTGAGCCACTGTGGCCGCCCTTACCGTAG
GGTCTCAGGGGATGGGGCCCAGGACCTGGATAGAGGACTGAGGAAGAGGA
CTTATTCCCTAACCACGCCTGCCAGGCAGCGGCAGGTACCTCAGCCTTCC
TCTGCCTCTCTAGATGAATACACTCTCATGAGGGCCACCTTCTCTGGTAG
TTCAGGTCGCCTCTGCCCATCCTTCCCTGCGTCCTCTCCCAAAGTGGCCT
ACAACCCTTACCCAGAGGACTATGGAGACATTGAGATTGGTTCTCACAAG
AGTTCCAGCAGTAACCTGGGGGCAGATGATGGCTACATGCCCATGACCCC
TGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAGCTGCAAGAGCGATGACT
ACATGCCCATGAGCCCCACAAGCGTGTCTGCTCCCAAGCAGATCCTGCAG
CCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGAGCAGCCGTGCCAGCACC
CCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAACGGAGGTGGCTACAAAG
CCAGCTCCCCAGCGGAGAGCTCCCCAGAAGACAGTGGGTACATGCGAATG
TGGTGTGGCTCCAAGCTGTCTATGGAGAACCCAGACCCTAAGCTACTCCC
CAACGGGGACTACCTCAACATGTCCCCCAGCGAGGCAGGCACTGCAGGGA
CCCCACCTGACTTCTCAGCAGCTTTGCGTGGAGGCAGTGAAGGCCTCAAA
GGCATCCCGGGCCACTGCTACAGCTCTTTGCCCCGCTCTTATAAGGCTCC
CTGTTCCTGCAGCGGAGACAATGACCAGTATGTGCTCATGAGCTCCCCTG
TGGGCCGGATCTTGGAAGAGGAGAGACTGGAGCCCCAGGCCACCCCAGGG
GCTGGCACCTTTGGGGCAGCTGGTGGTAGTCATACCCAGCCTCATCACTC
AGCAGTGCCTTCCTCCATGAGGCCGAGTGCCATCGGTGGCCGCCCTGAGG
GCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGCCTACACGCCTATCGCTA
GAGGGACTGCAGACCCTTCCCAGCATGCAAGAGTACCCTCTACCCACAGA
GCCCAAGAGCCCTGGCGAGTACATCAACATTGACTTTGGTGAGGCAGGTA
CCCGTCTGTCTCCGCCTGCCCCCCCACTACTGGCATCCGCGGCCTCATCT
TCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCATCCCTGGGTTCAGGAAC
CCCAGGCACCAGCAGCGACAGCCGGCAGCGCTCTCCACTCTCTGACTATA
TGAACCTGGACTTCAGTTCTCCCAAGTCCCCCAAGCCTAGCACCCGCAGT
GGGGACACAGTAGGCTCCATGGATGGCCTTCTCTCTCCAGAGGCTTCATC
CCCATACCCACCACTGCCCCCACGTCCTTCCACTTCCCCTTCCTCCTTAC
AGCAGCCTCTGCCACCTGCCCCGGGAGACCTATACCGCCTGCCTCCAGCA
TCAGCTGCCACTTCCCAGGGTCCCACTGCTGGCTCCTCAATGTCCTCCGA
GCCTGGGGATAATGGTGACTATACCGAGATGGCCTTTGGTGTGGCTGCAA
CCCCGCCACAACCTATCGTGGCACCTCCAAAGCCAGAAGGTGCCCGAGTG
GCCAGTCCCACATCGGGCTTGAAGCGGCTAAGTCTCATGGATCAGGTATC
TGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCCCCCTGACCCCCACCGGG
GTGCTAAGGTCATCCGTGCAGACCCACAGGGGGGACGTCGTCGCCACAGT
TCAGAGACCTTTTCCTCTACCACCACCGTCACCCCAGTGTCCCCATCCTT
TGCCCACAATTCCAAGCGCCACAATTCGGCCTCTGTGGAAAATGTCTCAC
TCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGGGAGGAGGTGATGAGCCG
CCCACATCCCCAGGACAGGCACAGCCCTTGGTGGCTGTGCCCCCAGTGCC
ACAGGCTAGGCCGTGGAACCCCGGTCAGCCCGGAGCTTTGATTGGCTGTC
CTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGACCTCCGTGGGTTTCCAG
AACGGCCTCAACTATATCGCCATCGATGTGAGAGGCGAGCAGGGGTCCTT
GGCGCAGTCTCAGCCGCAGCCAGGAGACAAGAACTCCTGGAGCCGGACCC
GTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAGGCTCTGGCGCCAGCGGA
GTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCCTCTGCCAGCACCTATGC
AAGCATCGACTTCCTGTCCCATCACTTGAAGGAAGCCACAGTCGTGAAAG
AGTGAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMASAPLPGPPASAGGDGPNLNNNNNNNNHSVRKCGYLRK
QKHGHKRFFVLRGPGTGGDEASAAGGSPPQPPRLEYYESEKKWRSKAGAP
KRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRAL
TDLVSEGRSGEGGSGTTGGSCSASLPGVLGGSAGAAGCDDNYGLVTPATA
VYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQL
MNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMK
ALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRS
RTDSLAATPPAAKCTSCRVRTASEGDGGAAGGAGTAGGRPMSVAGSPLSP
GPVRAPLSRSHTLSAGCGGRPSKVTLAPAGGALQHSRSMSMPVAHSPPAA
TSPGSLSSSSGHGSGSYPLPPGSHPHLPHPLHHPQGQRPSSGSASASGSP
SDPGFMSLDEYGSSPGDLRAFSSHRSNTPESIAETPPARDGSGGELYGYM
SMDRPLSHCGRPYRRVSGDGAQDLDRGLRKRTYSLTTPARQRQVPQPSSA
SLDEYTLMRATFSGSSGRLCPSFPASSPKVAYNPYPEDYGDIEIGSHKSS
SSNLGADDGYMPMTPGAALRSGGPNSCKSDDYMPMSPTSVSAPKQILQPR
LAAALPPSGAAVPAPPSGVGRTFPVNGGGYKASSPAESSPEDSGYMRMWC
GSKLSMENPDPKLLPNGDYLNMSPSEAGTAGTPPDFSAALRGGSEGLKGI
PGHCYSSLPRSYKAPCSCSGDNDQYVLMSSPVGRILEEERLEPQATPGAG
TFGAAGGSHTQPHHSAVPSSMRPSAIGGRPEGFLGQRCRAVRPTRLSLEG
LQTLPSMQEYPLPTEPKSPGEYINIDFGEAGTRLSPPAPPLLASAASSSS
LLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPSTRSGD
TVGSMDGLLSPEASSPYPPLPPRPSTSPSSLQQPLPPAPGDLYRLPPASA
ATSQGPTAGSSMSSEPGDNGDYTEMAFGVAATPPQPIVAPPKPEGARVAS
PTSGLKRLSLMDQVSGVEAFLQVSQPPDPHRGAKVIRADPQGGRRRHSSE
TFSSTTTVTPVSPSFAHNSKRHNSASVENVSLRKSSEGSSTLGGGDEPPT
SPGQAQPLVAVPPVPQARPWNPGQPGALIGCPGGSSSPMRRETSVGFQNG
LNYIAIDVRGEQGSLAQSQPQPGDKNSWSRTRSLGGLLGTVGGSGASGVC
GGPGTGALPSASTYASIDFLSHHLKEATVVKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4859
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.