cDNA Clone IRS2

• Plasmid: pCMV5-HA-1 IRS2
• Parent Plasmid: pCMV5
• Expressed: HA-IRS2
• Genbank: XP_357863
• Tag: HA
• Species: Mouse

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGGCTAG
CGCGCCCCTGCCTGGGCCCCCCGCGTCGGCGGGCGGGGACGGCCCGAACC
TCAATAACAACAACAACAACAACAACCACAGCGTGCGCAAGTGCGGCTAC
CTGCGCAAGCAGAAGCACGGCCACAAGCGCTTTTTCGTGTTGCGCGGCCC
CGGCACGGGCGGCGACGAGGCATCCGCGGCTGGGGGGTCGCCGCCGCAGC
CTCCGCGGCTGGAGTACTACGAGAGCGAGAAGAAGTGGAGGAGCAAGGCG
GGCGCGCCGAAGCGAGTGATCGCGCTCGACTGCTGTCTGAACATCAACAA
GCGCGCGGACGCCAAGCACAAGTACCTGATCGCCCTCTACACCAAGGACG
AGTACTTCGCTGTAGCGGCGGAGAACGAGCAGGAGCAGGAGGGCTGGTAC
CGCGCACTCACCGACTTGGTCAGCGAAGGCCGCTCTGGCGAGGGGGGCTC
GGGCACCACCGGAGGCTCTTGCAGCGCCTCTCTCCCGGGCGTCCTGGGCG
GCTCAGCGGGCGCCGCTGGCTGCGATGACAACTACGGGCTCGTGACACCC
GCCACGGCCGTCTACCGCGAGGTGTGGCAGGTGAACCTGAAACCTAAGGG
ACTGGGCCAGAGCAAGAACCTGACTGGTGTATACCGCCTATGCCTGTCTG
CGCGCACCATCGGCTTCGTGAAGCTCAATTGCGAACAGCCGTCGGTGACG
CTGCAGCTTATGAACATTCGCCGCTGCGGCCACTCGGACAGCTTCTTCTT
CATCGAGGTGGGCCGTTCGGCCGTCACCGGTCCCGGGGAGCTGTGGATGC
AAGCCGACGACTCGGTGGTGGCGCAGAACATCCATGAGACCATCCTAGAA
GCTATGAAGGCACTCAAAGAGCTCTTCGAGTTCCGGCCTCGCAGCAAGAG
TCAGTCGTCCGGGTCGTCAGCCACGCATCCCATCAGCGTGCCGGGCGCGC
GCCGCCACCACCACCTAGTCAACCTACCCCCTAGCCAGACCGGCCTGGTG
CGCCGCTCGCGCACTGACAGCCTGGCGGCCACCCCCCCAGCAGCCAAGTG
CACTTCGTGCCGGGTTCGTACGGCCAGCGAGGGCGACGGCGGCGCGGCAG
GCGGGGCCGGGACGGCAGGAGGCAGGCCGATGTCGGTGGCAGGGAGCCCC
CTGAGTCCCGGGCCGGTGCGCGCGCCCCTTAGCCGCTCGCACACCCTGAG
CGCCGGCTGCGGAGGCCGCCCGAGCAAAGTGACTCTGGCGCCGGCAGGGG
GAGCCCTGCAACACAGCCGCTCCATGTCCATGCCCGTGGCGCACTCACCT
CCTGCAGCCACCAGCCCAGGCAGCCTGTCCTCCAGCAGTGGGCACGGCTC
GGGCTCCTACCCGCTGCCACCTGGCTCCCACCCGCACCTGCCTCATCCAC
TGCATCACCCCCAAGGCCAGCGTCCGTCCAGCGGTAGTGCCTCCGCCTCG
GGCTCCCCCAGCGACCCGGGTTTCATGTCCCTTGACGAGTATGGCTCCAG
CCCTGGCGACCTGAGAGCCTTCAGTAGCCACAGGAGCAACACACCCGAGT
CAATAGCGGAGACCCCGCCAGCCAGAGATGGCAGTGGGGGCGAACTCTAT
GGGTACATGAGCATGGATAGACCCCTGAGCCACTGTGGCCGCCCTTACCG
TAGGGTCTCAGGGGATGGGGCCCAGGACCTGGATAGAGGACTGAGGAAGA
GGACTTATTCCCTAACCACGCCTGCCAGGCAGCGGCAGGTACCTCAGCCT
TCCTCTGCCTCTCTAGATGAATACACTCTCATGAGGGCCACCTTCTCTGG
TAGTTCAGGTCGCCTCTGCCCATCCTTCCCTGCGTCCTCTCCCAAAGTGG
CCTACAACCCTTACCCAGAGGACTATGGAGACATTGAGATTGGTTCTCAC
AAGAGTTCCAGCAGTAACCTGGGGGCAGATGATGGCTACATGCCCATGAC
CCCTGGGGCAGCCCTTAGGAGTGGTGGTCCCAATAGCTGCAAGAGCGATG
ACTACATGCCCATGAGCCCCACAAGCGTGTCTGCTCCCAAGCAGATCCTG
CAGCCACGCTTGGCAGCGGCCTTGCCCCCTTCCGGAGCAGCCGTGCCAGC
ACCCCCTTCAGGGGTGGGCAGGACCTTCCCAGTAAACGGAGGTGGCTACA
AAGCCAGCTCCCCAGCGGAGAGCTCCCCAGAAGACAGTGGGTACATGCGA
ATGTGGTGTGGCTCCAAGCTGTCTATGGAGAACCCAGACCCTAAGCTACT
CCCCAACGGGGACTACCTCAACATGTCCCCCAGCGAGGCAGGCACTGCAG
GGACCCCACCTGACTTCTCAGCAGCTTTGCGTGGAGGCAGTGAAGGCCTC
AAAGGCATCCCGGGCCACTGCTACAGCTCTTTGCCCCGCTCTTATAAGGC
TCCCTGTTCCTGCAGCGGAGACAATGACCAGTATGTGCTCATGAGCTCCC
CTGTGGGCCGGATCTTGGAAGAGGAGAGACTGGAGCCCCAGGCCACCCCA
GGGGCTGGCACCTTTGGGGCAGCTGGTGGTAGTCATACCCAGCCTCATCA
CTCAGCAGTGCCTTCCTCCATGAGGCCGAGTGCCATCGGTGGCCGCCCTG
AGGGCTTCCTGGGCCAGCGATGTCGGGCAGTGCGGCCTACACGCCTATCG
CTAGAGGGACTGCAGACCCTTCCCAGCATGCAAGAGTACCCTCTACCCAC
AGAGCCCAAGAGCCCTGGCGAGTACATCAACATTGACTTTGGTGAGGCAG
GTACCCGTCTGTCTCCGCCTGCCCCCCCACTACTGGCATCCGCGGCCTCA
TCTTCTTCACTGCTCTCAGCTAGTAGTCCTGCTTCATCCCTGGGTTCAGG
AACCCCAGGCACCAGCAGCGACAGCCGGCAGCGCTCTCCACTCTCTGACT
ATATGAACCTGGACTTCAGTTCTCCCAAGTCCCCCAAGCCTAGCACCCGC
AGTGGGGACACAGTAGGCTCCATGGATGGCCTTCTCTCTCCAGAGGCTTC
ATCCCCATACCCACCACTGCCCCCACGTCCTTCCACTTCCCCTTCCTCCT
TACAGCAGCCTCTGCCACCTGCCCCGGGAGACCTATACCGCCTGCCTCCA
GCATCAGCTGCCACTTCCCAGGGTCCCACTGCTGGCTCCTCAATGTCCTC
CGAGCCTGGGGATAATGGTGACTATACCGAGATGGCCTTTGGTGTGGCTG
CAACCCCGCCACAACCTATCGTGGCACCTCCAAAGCCAGAAGGTGCCCGA
GTGGCCAGTCCCACATCGGGCTTGAAGCGGCTAAGTCTCATGGATCAGGT
ATCTGGGGTGGAGGCTTTCCTTCAAGTCAGCCAGCCCCCTGACCCCCACC
GGGGTGCTAAGGTCATCCGTGCAGACCCACAGGGGGGACGTCGTCGCCAC
AGTTCAGAGACCTTTTCCTCTACCACCACCGTCACCCCAGTGTCCCCATC
CTTTGCCCACAATTCCAAGCGCCACAATTCGGCCTCTGTGGAAAATGTCT
CACTCAGGAAAAGCAGTGAAGGCAGCAGTACCCTGGGAGGAGGTGATGAG
CCGCCCACATCCCCAGGACAGGCACAGCCCTTGGTGGCTGTGCCCCCAGT
GCCACAGGCTAGGCCGTGGAACCCCGGTCAGCCCGGAGCTTTGATTGGCT
GTCCTGGAGGCAGCAGTTCTCCCATGCGCAGAGAGACCTCCGTGGGTTTC
CAGAACGGCCTCAACTATATCGCCATCGATGTGAGAGGCGAGCAGGGGTC
CTTGGCGCAGTCTCAGCCGCAGCCAGGAGACAAGAACTCCTGGAGCCGGA
CCCGTAGCCTTGGGGGGCTCCTCGGCACCGTCGGAGGCTCTGGCGCCAGC
GGAGTGTGTGGGGGTCCAGGCACTGGAGCTTTGCCCTCTGCCAGCACCTA
TGCAAGCATCGACTTCCTGTCCCATCACTTGAAGGAAGCCACAGTCGTGA
AAGAGTGAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMASAPLPGPPASAGGDGPNLNNNNNNNNHSVRKCGYLR
KQKHGHKRFFVLRGPGTGGDEASAAGGSPPQPPRLEYYESEKKWRSKAGA
PKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRA
LTDLVSEGRSGEGGSGTTGGSCSASLPGVLGGSAGAAGCDDNYGLVTPAT
AVYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQ
LMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAM
KALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRR
SRTDSLAATPPAAKCTSCRVRTASEGDGGAAGGAGTAGGRPMSVAGSPLS
PGPVRAPLSRSHTLSAGCGGRPSKVTLAPAGGALQHSRSMSMPVAHSPPA
ATSPGSLSSSSGHGSGSYPLPPGSHPHLPHPLHHPQGQRPSSGSASASGS
PSDPGFMSLDEYGSSPGDLRAFSSHRSNTPESIAETPPARDGSGGELYGY
MSMDRPLSHCGRPYRRVSGDGAQDLDRGLRKRTYSLTTPARQRQVPQPSS
ASLDEYTLMRATFSGSSGRLCPSFPASSPKVAYNPYPEDYGDIEIGSHKS
SSSNLGADDGYMPMTPGAALRSGGPNSCKSDDYMPMSPTSVSAPKQILQP
RLAAALPPSGAAVPAPPSGVGRTFPVNGGGYKASSPAESSPEDSGYMRMW
CGSKLSMENPDPKLLPNGDYLNMSPSEAGTAGTPPDFSAALRGGSEGLKG
IPGHCYSSLPRSYKAPCSCSGDNDQYVLMSSPVGRILEEERLEPQATPGA
GTFGAAGGSHTQPHHSAVPSSMRPSAIGGRPEGFLGQRCRAVRPTRLSLE
GLQTLPSMQEYPLPTEPKSPGEYINIDFGEAGTRLSPPAPPLLASAASSS
SLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPSTRSG
DTVGSMDGLLSPEASSPYPPLPPRPSTSPSSLQQPLPPAPGDLYRLPPAS
AATSQGPTAGSSMSSEPGDNGDYTEMAFGVAATPPQPIVAPPKPEGARVA
SPTSGLKRLSLMDQVSGVEAFLQVSQPPDPHRGAKVIRADPQGGRRRHSS
ETFSSTTTVTPVSPSFAHNSKRHNSASVENVSLRKSSEGSSTLGGGDEPP
TSPGQAQPLVAVPPVPQARPWNPGQPGALIGCPGGSSSPMRRETSVGFQN
GLNYIAIDVRGEQGSLAQSQPQPGDKNSWSRTRSLGGLLGTVGGSGASGV
CGGPGTGALPSASTYASIDFLSHHLKEATVVKE*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4858
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA.