cDNA Clone NCC

• Plasmid: pCMV5-HA-1 SLC12A3 (hNCC) T46A T50A T55A S91A
• Parent Plasmid: pCMV5
• Expressed: HA-SLC12A3 (hNCC) T46A T50A T55A S91A
• Genbank: P55017
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGGCAGA
ACTGCCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGCT
TCACCATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCC
TATGACAGCAGCCACCCCAGCCACCTGGCCCACAGCAGCGCCTTCTGCAT
GCGCGCCTTTGGCTATAACACGATCGATGTGGTGCCCACATATGAGCACT
ATGCCAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACTG
GCTGACCTGCACGCCTTCCTCAAGCAGGAAGGCAGACACCTGCATGCCCT
GGCCTTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAGG
GCGAGGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTTC
GGCTGGGTCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGGGGCGT
GATCCTCTACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGGCATCGTCC
TGACCTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCATCACAGGC
CTCTCCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGTGGCAC
CTACTTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCG
GCCTCATTTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGCACACGGTG
GGCTTTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCACCCAT
CGTGGACCCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGTCACTG
TGCTGCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAGGCCCAG
GTGCTGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTAGTGGG
GACGCTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCT
ACCGGGCGGACATTTTTGTCCAAAACTTGGTGCCTGACTGGCGGGGTCCA
GATGGCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCCACAGG
CATCCTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGCTATAG
CCATCCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTAC
CTGGCCATCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGATGCCTCTGG
GGTCCTGAATGACACAGTGACCCCTGGCTGGGGTGCCTGCGAGGGGCTGG
CCTGCAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGC
CACTACGGCCTCATCAACTATTACCAGACCATGAGCATGGTGTCAGGCTT
CGCGCCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTCTGCCC
TGGCCTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTTGCGAGGAC
CAGCTGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAGAACAA
GGAGCCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCA
TCATCATCGCTGAGCTCAACACCATAGCCCCCATCATTTCCAACTTCTTC
CTCTGCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATCAC
CAACTCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGG
CGCTGTTTGGGGCTATCATCTCCGTGGTCATCATGTTCCTCCTCACCTGG
TGGGCGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTGCTCTATGT
CATCTACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGCT
CCTACAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGAC
CACATCAAGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGGCCCCCCAA
CTTCCGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGGAACCTCA
GCCTGATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGAGGATG
CCTGAGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAG
GAAGATCAAGGCCTTCTACTCGGATGTCATTGCTGAGGACCTCCGCAGAG
GCGTCCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAAC
ATTCTGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCAC
AGTGGAAGACTACATTGGCATCCTCCATGATGCCTTTGATTTCAACTATG
GCGTGTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTCCAAGATGATG
CAGGCGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGC
CAGCGCCAGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACCA
CCATCTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTC
TTTGACGATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTTGGCCGCAA
GAGGAGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGGCCAGATTA
ACAGGATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCAAGTTC
CGACTGGGATTCCATGAAGTCCACATCCTCCCTGACATCAACCAGAACCC
TCGGGCTGAGCACACCAAGAGGTTTGAGGACATGATTGCACCCTTCCGTC
TGAATGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGGCGGGAC
TGCCCCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGTC
CCTTCGGCAGGTGAGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACG
CTGCTCTCATCGTCATCACTTTGCCCATAGGGAGGAAGGGGAAGTGCCCC
AGCTCGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTCAGACC
TCCAGTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTTTTACT
GCCAGTAAGCGGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYD
SSHPSHLAHSSAFCMRAFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLAD
LHAFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGW
VKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLS
ISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGF
AETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVL
FFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDG
TFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLA
ISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHY
GLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQL
YPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLC
SYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWA
ALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHI
KNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPE
LQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNIL
VVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQA
HINPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFD
DGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRL
GFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCP
WKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSS
LYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4853
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA.