cDNA Clone HLA-B

• Plasmid: pGEX6P-1-HLA-B
• Parent Plasmid: pGEX6P1
• Expressed: GST-HLA-B
• Genbank: NM_006709
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCCCCGGGC
TGCAGCGACCAATGTGGAATTCGCCCTTCGAGGGGGAGGCCCCCGCTGAG
ATGGGGGCGCTGCTGCTGGAGAAGGAAACCAGAGGAGCCACCGAGAGAGT
TCATGGCTCTTTGGGGGACACCCCTCGTAGTGAAGAAACCCTGCCCAAGG
CCAACCCCGACTCCCTGGAGCCTGCTGGCCCCTCATCTCCAGCCTCTGTC
ACTGTCACTGTTGGTGATGAGGGGGCTGACACCCCTGTAGGGGCTACACC
ACTCATTGGGGATGAATCTGAGAATCTTGAGGGAGATGGGGACCTCCGTG
GGGGCCGGATCCTGCTGGGCCATGCCACAAAGTCATTCCCCTCTTCCCCC
AGCAAGGGGGGTTCCTGTCCTAGCCGGGCCAAGATGTCAATGACAGGGGC
GGGAAAATCACCTCCATCTGTCCAGAGTTTGGCTATGAGGCTACTGAGTA
TGCCAGGAGCCCAGGGAGCTGCAGCAGCAGGGTCTGAACCCCCTCCAGCC
ACCACGAGCCCAGAGGGACAGCCCAAGGTCCACCGAGCCCGCAAAACCAT
GTCCAAACCAGGAAATGGACAGCCCCCGGTCCCTGAGAAGCGGCCCCCTG
AAATACAGCATTTCCGCATGAGTGATGATGTCCACTCACTGGGAAAGGTG
ACCTCAGATCTGGCCAAAAGGAGGAAGCTGAACTCAGGAGGTGGCCTGTC
GGAGGAGTTAGGTTCTGCCCGGCGTTCAGGAGAAGTGACCCTGACGAAAG
GGGACCCCGGGTCCCTGGAGGAGTGGGAGACGGTGGTGGGTGATGACTTC
AGTCTCTACTATGATTCCTACTCTGTGGATGAGCGCGTGGACTCCGACAG
CAAGTCTGAAGTTGAAGCTCTAACTGAACAACTAAGTGAAGAGGAGGAGG
AGGAAGAGGAGGAAGAAGAAGAAGAGGAAGAGGAGGAGGAAGAGGAAGAA
GAAGAGGAAGATGAGGAGTCAGGGAATCAGTCAGATAGGAGTGGTTCCAG
TGGCCGGCGCAAGGCCAAGAAGAAATGGCGAAAAGACAGCCCATGGGTGA
AGCCGTCTCGGAAACGGCGCAAGCGGGAGCCTCCGCGGGCCAAGGAGCCA
CGAGGAGTGAATGGTGTGGGCTCCTCAGGCCCCAGTGAGTACATGGAGGT
CCCTCTGGGGTCCCTGGAGCTGCCCAGCGAGGGGACCCTCTCCCCCAACC
ACGCTGGGGTGTCCAATGACACATCTTCGCTGGAGACAGAGCGAGGGTTT
GAGGAGTTGCCCCTGTGCAGCTGCCGCATGGAGGCACCCAAGATTGACCG
CATCAGCGAGAGGGCGGGGCACAAGTGCATGGCCACTGAGAGTGTGGACG
GAGAGCTGTCAGGCTGCAATGCCGCCATCCTCAAGCGGGAGACCATGAGG
CCATCCAGCCGTGTGGCCCTGATGGTGCTCTGTGAGACCCACCGCGCCCG
CATGGTCAAACACCACTGCTGCCCGGGCTGCGGCTACTTCTGCACGGCGG
GCACCTTCCTGGAGTGCCACCCTGACTTCCGTGTGGCCCACCGCTTCCAC
AAGGCCTGTGTGTCTCAGCTGAATGGGATGGTCTTCTGTCCCCACTGTGG
GGAGGATGCTTCTGAAGCTCAAGAGGTGACCATCCCCCGGGGTGACGGGG
TGACCCCACCGGCCGGCACTGCAGCTCCTGCACCCCCACCCCTGTCCCAG
GATGTCCCCGGGAGAGCAGACACTTCTCAGCCCAGTGCCCGGATGCGAGG
GCATGGGGAACCCCGGCGCCCGCCCTGCGATCCCCTGGCTGACACCATTG
ACAGCTCAGGGCCCTCCCTGACCCTGCCCAATGGGGGCTGCCTTTCAGCC
GTGGGGCTGCCACTGGGGCCAGGCCGGGAGGCCCTGGAAAAGGCCCTGGT
CATCCAGGAGTCAGAGAGGCGGAAGAAGCTCCGTTTCCACCCTCGGCAGT
TGTACCTGTCCGTGAAGCAGGGCGAGCTGCAGAAGGTGATCCTGATGCTG
TTGGACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCAC
GCCCCTGCATGCAGCCGCCCAGAAGGGCTCCGTGGAGATCTGCCATGTGC
TGCTGCAGGCTGGAGCCAACATAAATGCAGTGGACAAACAGCAGCGGACG
CCACTGATGGAGGCCGTGGTGAACAACCACCTGGAGGTAGCCCGTTACAT
GGTGCAGCGTGGTGGCTGTGTCTATAGCAAGGAGGAGGACGGTTCCACCT
GCCTCCACCACGCAGCCAAAATCGGGAACTTGGAGATGGTCAGCCTGCTG
CTGAGCACAGGACAGGTGGACGTCAACGCCCAGGACAGTGGGGGGTGGAC
GCCCATCATCTGGGCTGCAGAGCACAAGCACATCGAGGTGATCCGCATGC
TACTGACGCGGGGCGCCGACGTCACCCTCACTGACAACGAGGAGAACATC
TGCCTGCACTGGGCCTCCTTCACGGGCAGCGCCGCCATCGCCGAAGTCCT
TCTGAATGCGCGCTGTGACCTCCATGCTGTCAACTACCATGGGGACACCC
CCCTGCACATCGCAGCTCGGGAGAGCTACCATGACTGCGTGCTGTTATTC
CTGTCACGTGGGGCCAACCCTGAGCTGCGGAACAAAGAGGGGGACACAGC
ATGGGACCTGACTCCCGAGCGCTCCGACGTGTGGTTTGCGCTTCAACTCA
ACCGCAAGCTCCGACTTGGGGTGGGAAATCGGGCCATCCGCACAGAGAAG
ATCATCTGCCGGGACGTGGCTCGGGGCTATGAGAACGTGCCCATTCCCTG
TGTCAACGGTGTGGATGGGGAGCCCTGCCCTGAGGATTACAAGTACATCT
CAGAGAACTGCGAGACGTCCACCATGAACATCGATCGCAACATCACCCAC
CTGCAGCACTGCACGTGTGTGGACGACTGCTCTAGCTCCAACTGCCTGTG
CGGCCAGCTCAGCATCCGGTGCTGGTATGACAAGGATGGGCGATTGCTCC
AGGAATTTAACAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCG
TGCTCATGCTGGAGAAACTGCAAGAACCGGGTCGTACAGAGTGGCATCAA
GGTGCGGCTACAGCTCTACCGAACAGCCAAGATGGGCTGGGGGGTCCGCG
CCCTGCAGACCATCCCACAGGGGACCTTCATCTGCGAGTATGTCGGGGAG
CTGATCTCTGATGCTGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTT
CGACTTAGACAACAAGGATGGAGAGGTGTACTGCATAGATGCCCGTTACT
ATGGCAACATCAGCCGCTTCATCAACCACCTGTGTGACCCCAACATCATT
CCCGTCCGGGTCTTCATGCTGCACCAAGACCTGCGATTTCCACGCATCGC
CTTCTTCAGTTCCCGAGACATCCGGACTGGGGAGGAGCTAGGGTTTGACT
ATGGCGACCGCTTCTGGGACATCAAAAGCAAATATTTCACCTGCCAATGT
GGCTCTGAGAAGTGCAAGCACTCAGCCGAAGCCATTGCCCTGGAGCAGAG
CCGTCTGGCCCGCCTGGACCCACACCCTGAGCTGCTGCCCGAGCTCGGCT
CCCTGCCCCCTGTCAACACATGAGTCGAC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPGLQRPMWNSPFEGEAPAE
MGALLLEKETRGATERVHGSLGDTPRSEETLPKANPDSLEPAGPSSPASV
TVTVGDEGADTPVGATPLIGDESENLEGDGDLRGGRILLGHATKSFPSSP
SKGGSCPSRAKMSMTGAGKSPPSVQSLAMRLLSMPGAQGAAAAGSEPPPA
TTSPEGQPKVHRARKTMSKPGNGQPPVPEKRPPEIQHFRMSDDVHSLGKV
TSDLAKRRKLNSGGGLSEELGSARRSGEVTLTKGDPGSLEEWETVVGDDF
SLYYDSYSVDERVDSDSKSEVEALTEQLSEEEEEEEEEEEEEEEEEEEEE
EEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEP
RGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGF
EELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMR
PSSRVALMVLCETHRARMVKHHCCPGCGYFCTAGTFLECHPDFRVAHRFH
KACVSQLNGMVFCPHCGEDASEAQEVTIPRGDGVTPPAGTAAPAPPPLSQ
DVPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPSLTLPNGGCLSA
VGLPLGPGREALEKALVIQESERRKKLRFHPRQLYLSVKQGELQKVILML
LDNLDPNFQSDQQSKRTPLHAAAQKGSVEICHVLLQAGANINAVDKQQRT
PLMEAVVNNHLEVARYMVQRGGCVYSKEEDGSTCLHHAAKIGNLEMVSLL
LSTGQVDVNAQDSGGWTPIIWAAEHKHIEVIRMLLTRGADVTLTDNEENI
CLHWASFTGSAAIAEVLLNARCDLHAVNYHGDTPLHIAARESYHDCVLLF
LSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEK
IICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITH
LQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQA
CSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGE
LISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNII
PVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQC
GSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT*VD

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4844
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST. This protein differs from the accession given in that this one has a 34 amino acids insertion from 372-405. The first 12 amino acids are from pSC-B vector.