cDNA Clone HLA-B

• Plasmid: pCMV5-FLAG-1 HLA-B
• Parent Plasmid: pCMV5
• Expressed: FLAG-HLA-B
• Genbank: NM_006709
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCCCCGGGCTGCA
GCGACCAATGTGGAATTCGCCCTTCGAGGGGGAGGCCCCCGCTGAGATGG
GGGCGCTGCTGCTGGAGAAGGAAACCAGAGGAGCCACCGAGAGAGTTCAT
GGCTCTTTGGGGGACACCCCTCGTAGTGAAGAAACCCTGCCCAAGGCCAA
CCCCGACTCCCTGGAGCCTGCTGGCCCCTCATCTCCAGCCTCTGTCACTG
TCACTGTTGGTGATGAGGGGGCTGACACCCCTGTAGGGGCTACACCACTC
ATTGGGGATGAATCTGAGAATCTTGAGGGAGATGGGGACCTCCGTGGGGG
CCGGATCCTGCTGGGCCATGCCACAAAGTCATTCCCCTCTTCCCCCAGCA
AGGGGGGTTCCTGTCCTAGCCGGGCCAAGATGTCAATGACAGGGGCGGGA
AAATCACCTCCATCTGTCCAGAGTTTGGCTATGAGGCTACTGAGTATGCC
AGGAGCCCAGGGAGCTGCAGCAGCAGGGTCTGAACCCCCTCCAGCCACCA
CGAGCCCAGAGGGACAGCCCAAGGTCCACCGAGCCCGCAAAACCATGTCC
AAACCAGGAAATGGACAGCCCCCGGTCCCTGAGAAGCGGCCCCCTGAAAT
ACAGCATTTCCGCATGAGTGATGATGTCCACTCACTGGGAAAGGTGACCT
CAGATCTGGCCAAAAGGAGGAAGCTGAACTCAGGAGGTGGCCTGTCGGAG
GAGTTAGGTTCTGCCCGGCGTTCAGGAGAAGTGACCCTGACGAAAGGGGA
CCCCGGGTCCCTGGAGGAGTGGGAGACGGTGGTGGGTGATGACTTCAGTC
TCTACTATGATTCCTACTCTGTGGATGAGCGCGTGGACTCCGACAGCAAG
TCTGAAGTTGAAGCTCTAACTGAACAACTAAGTGAAGAGGAGGAGGAGGA
AGAGGAGGAAGAAGAAGAAGAGGAAGAGGAGGAGGAAGAGGAAGAAGAAG
AGGAAGATGAGGAGTCAGGGAATCAGTCAGATAGGAGTGGTTCCAGTGGC
CGGCGCAAGGCCAAGAAGAAATGGCGAAAAGACAGCCCATGGGTGAAGCC
GTCTCGGAAACGGCGCAAGCGGGAGCCTCCGCGGGCCAAGGAGCCACGAG
GAGTGAATGGTGTGGGCTCCTCAGGCCCCAGTGAGTACATGGAGGTCCCT
CTGGGGTCCCTGGAGCTGCCCAGCGAGGGGACCCTCTCCCCCAACCACGC
TGGGGTGTCCAATGACACATCTTCGCTGGAGACAGAGCGAGGGTTTGAGG
AGTTGCCCCTGTGCAGCTGCCGCATGGAGGCACCCAAGATTGACCGCATC
AGCGAGAGGGCGGGGCACAAGTGCATGGCCACTGAGAGTGTGGACGGAGA
GCTGTCAGGCTGCAATGCCGCCATCCTCAAGCGGGAGACCATGAGGCCAT
CCAGCCGTGTGGCCCTGATGGTGCTCTGTGAGACCCACCGCGCCCGCATG
GTCAAACACCACTGCTGCCCGGGCTGCGGCTACTTCTGCACGGCGGGCAC
CTTCCTGGAGTGCCACCCTGACTTCCGTGTGGCCCACCGCTTCCACAAGG
CCTGTGTGTCTCAGCTGAATGGGATGGTCTTCTGTCCCCACTGTGGGGAG
GATGCTTCTGAAGCTCAAGAGGTGACCATCCCCCGGGGTGACGGGGTGAC
CCCACCGGCCGGCACTGCAGCTCCTGCACCCCCACCCCTGTCCCAGGATG
TCCCCGGGAGAGCAGACACTTCTCAGCCCAGTGCCCGGATGCGAGGGCAT
GGGGAACCCCGGCGCCCGCCCTGCGATCCCCTGGCTGACACCATTGACAG
CTCAGGGCCCTCCCTGACCCTGCCCAATGGGGGCTGCCTTTCAGCCGTGG
GGCTGCCACTGGGGCCAGGCCGGGAGGCCCTGGAAAAGGCCCTGGTCATC
CAGGAGTCAGAGAGGCGGAAGAAGCTCCGTTTCCACCCTCGGCAGTTGTA
CCTGTCCGTGAAGCAGGGCGAGCTGCAGAAGGTGATCCTGATGCTGTTGG
ACAACCTGGACCCCAACTTCCAGAGCGACCAGCAGAGCAAGCGCACGCCC
CTGCATGCAGCCGCCCAGAAGGGCTCCGTGGAGATCTGCCATGTGCTGCT
GCAGGCTGGAGCCAACATAAATGCAGTGGACAAACAGCAGCGGACGCCAC
TGATGGAGGCCGTGGTGAACAACCACCTGGAGGTAGCCCGTTACATGGTG
CAGCGTGGTGGCTGTGTCTATAGCAAGGAGGAGGACGGTTCCACCTGCCT
CCACCACGCAGCCAAAATCGGGAACTTGGAGATGGTCAGCCTGCTGCTGA
GCACAGGACAGGTGGACGTCAACGCCCAGGACAGTGGGGGGTGGACGCCC
ATCATCTGGGCTGCAGAGCACAAGCACATCGAGGTGATCCGCATGCTACT
GACGCGGGGCGCCGACGTCACCCTCACTGACAACGAGGAGAACATCTGCC
TGCACTGGGCCTCCTTCACGGGCAGCGCCGCCATCGCCGAAGTCCTTCTG
AATGCGCGCTGTGACCTCCATGCTGTCAACTACCATGGGGACACCCCCCT
GCACATCGCAGCTCGGGAGAGCTACCATGACTGCGTGCTGTTATTCCTGT
CACGTGGGGCCAACCCTGAGCTGCGGAACAAAGAGGGGGACACAGCATGG
GACCTGACTCCCGAGCGCTCCGACGTGTGGTTTGCGCTTCAACTCAACCG
CAAGCTCCGACTTGGGGTGGGAAATCGGGCCATCCGCACAGAGAAGATCA
TCTGCCGGGACGTGGCTCGGGGCTATGAGAACGTGCCCATTCCCTGTGTC
AACGGTGTGGATGGGGAGCCCTGCCCTGAGGATTACAAGTACATCTCAGA
GAACTGCGAGACGTCCACCATGAACATCGATCGCAACATCACCCACCTGC
AGCACTGCACGTGTGTGGACGACTGCTCTAGCTCCAACTGCCTGTGCGGC
CAGCTCAGCATCCGGTGCTGGTATGACAAGGATGGGCGATTGCTCCAGGA
ATTTAACAAGATTGAGCCTCCGCTGATTTTCGAGTGTAACCAGGCGTGCT
CATGCTGGAGAAACTGCAAGAACCGGGTCGTACAGAGTGGCATCAAGGTG
CGGCTACAGCTCTACCGAACAGCCAAGATGGGCTGGGGGGTCCGCGCCCT
GCAGACCATCCCACAGGGGACCTTCATCTGCGAGTATGTCGGGGAGCTGA
TCTCTGATGCTGAGGCTGATGTGAGAGAGGATGATTCTTACCTCTTCGAC
TTAGACAACAAGGATGGAGAGGTGTACTGCATAGATGCCCGTTACTATGG
CAACATCAGCCGCTTCATCAACCACCTGTGTGACCCCAACATCATTCCCG
TCCGGGTCTTCATGCTGCACCAAGACCTGCGATTTCCACGCATCGCCTTC
TTCAGTTCCCGAGACATCCGGACTGGGGAGGAGCTAGGGTTTGACTATGG
CGACCGCTTCTGGGACATCAAAAGCAAATATTTCACCTGCCAATGTGGCT
CTGAGAAGTGCAAGCACTCAGCCGAAGCCATTGCCCTGGAGCAGAGCCGT
CTGGCCCGCCTGGACCCACACCCTGAGCTGCTGCCCGAGCTCGGCTCCCT
GCCCCCTGTCAACACATGAGTCGAC

Amino Acid Sequence

MDYKDDDDKGSPGLQRPMWNSPFEGEAPAEMGALLLEKETRGATERVHGS
LGDTPRSEETLPKANPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIG
DESENLEGDGDLRGGRILLGHATKSFPSSPSKGGSCPSRAKMSMTGAGKS
PPSVQSLAMRLLSMPGAQGAAAAGSEPPPATTSPEGQPKVHRARKTMSKP
GNGQPPVPEKRPPEIQHFRMSDDVHSLGKVTSDLAKRRKLNSGGGLSEEL
GSARRSGEVTLTKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSE
VEALTEQLSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRR
KAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLG
SLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISE
RAGHKCMATESVDGELSGCNAAILKRETMRPSSRVALMVLCETHRARMVK
HHCCPGCGYFCTAGTFLECHPDFRVAHRFHKACVSQLNGMVFCPHCGEDA
SEAQEVTIPRGDGVTPPAGTAAPAPPPLSQDVPGRADTSQPSARMRGHGE
PRRPPCDPLADTIDSSGPSLTLPNGGCLSAVGLPLGPGREALEKALVIQE
SERRKKLRFHPRQLYLSVKQGELQKVILMLLDNLDPNFQSDQQSKRTPLH
AAAQKGSVEICHVLLQAGANINAVDKQQRTPLMEAVVNNHLEVARYMVQR
GGCVYSKEEDGSTCLHHAAKIGNLEMVSLLLSTGQVDVNAQDSGGWTPII
WAAEHKHIEVIRMLLTRGADVTLTDNEENICLHWASFTGSAAIAEVLLNA
RCDLHAVNYHGDTPLHIAARESYHDCVLLFLSRGANPELRNKEGDTAWDL
TPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNG
VDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQL
SIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRL
QLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLD
NKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFS
SRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLA
RLDPHPELLPELGSLPPVNT*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4843
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG. This protein differs from the accession given in that this one has a 34 amino acids insertion from 372-405. The first 12 amino acids are from pSC-B vector.