cDNA Clone Optineurin

• Plasmid: pCMV5-HA-1 Optineurin E50K E478A
• Parent Plasmid: pCMV5
• Expressed: HA-Optineurin E50K E478A
• Genbank: AF420371
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGATCCATGTCCCA
TCAACCTCTCAGCTGCCTCACTGAAAAGGAGGACAGCCCCAGTGAAAGCA
CAGGAAATGGACCCCCCCACCTGGCCCACCCAAACCTGGACACGTTTACC
CCGGAGGAGCTGCTGCAGCAGATGAAAGAGCTCCTGACCAAGAACCACCA
GCTGAAAGAAGCCATGAAGCTAAATAATCAAGCCATGAAAGGGAGATTTG
AGGAGCTTTCGGCCTGGACAGAGAAACAGAAGGAAGAACGCCAGTTTTTT
GAGATACAGAGCAAAGAAGCAAAAGAGCGTCTAATGGCCTTGAGTCATGA
GAATGAGAAATTGAAGGAAGAGCTTGGAAAACTAAAAGGGAAATCAGAAA
GGTCATCTGAGGACCCCACTGATGACTCCAGGCTTCCCAGGGCCGAAGCG
GAGCAGGAAAAGGACCAGCTCAGGACCCAGGTGGTGAGGCTACAAGCAGA
GAAGGCAGACCTGTTGGGCATCGTGTCTGAACTGCAGCTCAAGCTGAACT
CCAGCGGCTCCTCAGAAGATTCCTTTGTTGAAATTAGGATGGCTGAAGGA
GAAGCAGAAGGGTCAGTAAAAGAAATCAAGCATAGTCCTGGGCCCACGAG
AACAGTCTCCACTGGCACGGCATTGTCTAAATATAGGAGCAGATCTGCAG
ATGGGGCCAAGAATTACTTCGAACATGAGGAGTTAACTGTGAGCCAGCTC
CTGCTGTGCCTAAGGGAAGGGAATCAGAAGGTGGAGAGACTTGAAGTTGC
ACTCAAGGAGGCCAAAGAAAGAGTTTCAGATTTTGAAAAGAAAACAAGTA
ATCGTTCTGAGATTGAAACCCAGACAGAGGGGAGCACAGAGAAAGAGAAT
GATGAAGAGAAAGGCCCGGAGACTGTTGGAAGCGAAGTGGAAGCACTGAA
CCTCCAGGTGACATCTCTGTTTAAGGAGCTTCAAGAGGCTCATACAAAAC
TCAGCGAAGCTGAGCTAATGAAGAAGAGACTTCAAGAAAAGTGTCAGGCC
CTTGAAAGGAAAAATTCTGCAATTCCATCAGAGTTGAATGAAAAGCAAGA
GCTTGTTTATACTAACAAAAAGTTAGAGCTACAAGTGGAAAGCATGCTAT
CAGAAATCAAAATGGAACAGGCTAAAACAGAGGATGAAAAGTCCAAATTA
ACTGTGCTACAGATGACACACAACAAGCTTCTTCAAGAACATAATAATGC
ATTGAAAACAATTGAGGAACTAACAAGAAAAGAGTCAGAAAAAGTGGACA
GGGCAGTGCTGAAGGAACTGAGTGAAAAACTGGAACTGGCAGAGAAGGCT
CTGGCTTCCAAACAGCTGCAAATGGATGAAATGAAGCAAACCATTGCCAA
GCAGGAAGAGGACCTGGAAACCATGACCATCCTCAGGGCTCAGATGGAAG
TTTACTGTTCTGATTTTCATGCTGCAAGAGCAGCGAGAGAGAAAATTCAT
GAGGAAAAGGAGCAACTGGCATTGCAGCTGGCAGTTCTGCTGAAAGAGAA
TGATGCTTTCGAAGACGGAGGCAGGCAGTCCTTGATGGAGATGCAGAGTC
GTCATGGGGCGAGAACAAGTGACTCTGACCAGCAGGCTTACCTTGTTCAA
AGAGGAGCTGAGGACAGGGACTGGCGGCAACAGCGGAATATTCCGATTCA
TTCCTGCCCCAAGTGTGGAGAGGTTCTGCCTGACATAGACACGTTACAGA
TTCACGTGATGGATTGCATCATTTAAGCGGCCGCG

Amino Acid Sequence

MYPYDVPDYAGSMSHQPLSCLTEKEDSPSESTGNGPPHLAHPNLDTFTPE
ELLQQMKELLTKNHQLKEAMKLNNQAMKGRFEELSAWTEKQKEERQFFEI
QSKEAKERLMALSHENEKLKEELGKLKGKSERSSEDPTDDSRLPRAEAEQ
EKDQLRTQVVRLQAEKADLLGIVSELQLKLNSSGSSEDSFVEIRMAEGEA
EGSVKEIKHSPGPTRTVSTGTALSKYRSRSADGAKNYFEHEELTVSQLLL
CLREGNQKVERLEVALKEAKERVSDFEKKTSNRSEIETQTEGSTEKENDE
EKGPETVGSEVEALNLQVTSLFKELQEAHTKLSEAELMKKRLQEKCQALE
RKNSAIPSELNEKQELVYTNKKLELQVESMLSEIKMEQAKTEDEKSKLTV
LQMTHNKLLQEHNNALKTIEELTRKESEKVDRAVLKELSEKLELAEKALA
SKQLQMDEMKQTIAKQEEDLETMTILRAQMEVYCSDFHAARAAREKIHEE
KEQLALQLAVLLKENDAFEDGGRQSLMEMQSRHGARTSDSDQQAYLVQRG
AEDRDWRQQRNIPIHSCPKCGEVLPDIDTLQIHVMDCII*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4804
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA.