cDNA Clone MLEL1

• Plasmid: pCMV5-FLAG-1 MLEL1
• Parent Plasmid: pCMV5
• Expressed: FLAG-MLEL1
• Genbank: AF217190
• Tag: FLAG
• Species: Human

Sequence of Insert

GCCACCATGGACTACAAGGACGATGACGATAAGGGATCCATGAGTTATGA
CTACCATCAGAACTGGGGCCGTGATGGGGGTCCCCGCAGCTCCGGTGGGG
GCTATGGAGGGGGGCCAGCAGGGGGTCATGGAGGTAACCGAGGCTCCGGA
GGAGGCGGCGGCGGCGGAGGGGGTGGTCGAGGCGGCAGGGGCCGGCATCC
CGGGCACCTGAAAGGCCGCGAAATCGGCATGTGGTACGCGAAAAAACAGG
GGCAGAAGAACAAGGAAGCGGAGAGGCAAGAGAGAGCTGTAGTACACATG
GATGAACGACGAGAAGAACAAATTGTACAGTTACTGAATTCTGTTCAAGC
GAAGAATGATAAAGAGTCAGAAGCACAGATATCCTGGTTTGCTCCTGAGG
ATCATGGATACGGTACTGAAGTTTCTACTAAGAACACACCATGCTCAGAG
AACAAACTTGACATCCAGGAAAAGAAGTTGATAAATCAAGAAAAAAAAAT
GTTTAGAATCAGGAACAGATCATATATTGACCGAGATTCTGAGTATCTCT
TGCAAGAAAATGAACCAGATGGAACTTTAGACCAAAAATTATTGGAAGAT
TTACAAAAGAAAAAAAATGACCTTCGGTATATTGAAATGCAGCATTTCAG
AGAAAAGCTGCCTTCGTATGGAATGCAAAAGGAATTGGTAAATTTAATTG
ATAACCATCAGGTAACAGTAATAAGTGGTGAAACTGGTTGTGGCAAAACC
ACTCAAGTTACTCAGTTCATTTTGGATAACTACATTGAAAGAGGAAAAGG
ATCTGCTTGCAGAATAGTTTGTACTCAGCCAAGAAGAATTAGTGCCATTT
CAGTTGCGGAAAGAGTAGCTGCAGAAAGGGCAGAATCTTGTGGCAGTGGT
AATAGTACTGGATATCAAATTCGTCTCCAGAGTCGGTTGCCAAGGAAACA
GGGTTCTATCTTATACTGTACAACAGGAATCATCCTTCAGTGGCTCCAGT
CAGACCCGTATTTGTCCAGTGTTAGTCATATCGTACTTGATGAAATCCAT
GAAAGAAATCTGCAGTCAGATGTTTTAATGACTGTTGTTAAAGACCTTCT
CAATTTTCGATCTGACTTGAAAGTAATATTGATGAGTGCAACATTGAATG
CAGAAAAGTTTTCAGAATATTTTGGTAACTGTCCAATGATACATATACCT
GGTTTTACCTTTCCGGTTGTGGAATATCTTTTGGAAGATGTAATTGAAAA
AATAAGGTATGTTCCAGAACAAAAAGAACACAGATGCCAGTTTAAGAGGG
GTTTCATGCAAGGGCATGTAAATAGACAAGAAAAAGAAGAAAAAGAAGCA
ATATATAAAGAACGTTGGCCAGATTATGTAAGGGAACTGCGAAGAAGGTA
TTCTGCAAGTACTGTAGATGTTATAGAAATGATGGAGGATGATAAAGTTG
ATCTGAATTTGATTGTTGCCCTCATCCGATACATTGTTTTGGAAGAAGAG
GATGGTGCGATACTGGTCTTTCTGCCAGGCTGGGACAATATCAGCACTTT
ACATGATCTCTTGATGTCACAAGTAATGTTTAAATCAGATAAATTTTTAA
TTATACCTTTACATTCACTGATGCCTACAGTTAACCAGACACAGGTGTTT
AAAAGAACCCCTCCTGGTGTTCGGAAAATAGTAATTGCTACCAACATTGC
GGAGACTAGCATTACCATAGATGATGTCGTTTATGTGATAGATGGAGGAA
AAATAAAAGAGACGCATTTTGATACTCAGAACAATAACAGTACAATGTCC
GCTGAGTGGGTTAGTAAAGCTAATGCCAAACAGAGAAAAGGTCGAGCTGG
AAGAGTTCAACCTGGTCATTGCTATCATCTGTATAATGGTCTTAGAGCAA
GTCTTCTAGATGACTATCAACTGCCAGAAATTTTGAGAACTCCTTTGGAA
GAACTTTGTTTACAAATAAAGATTTTAAGGCTAGGTGGAATTGCTTATTT
TCTGAGTAGATTAATGGACCCACCATCAAATGAGGCAGTGTTACTCTCCA
TAAGACACCTGATGGAGCTGAACGCTTTGGATAAACAAGAAGAATTGACA
CCTCTTGGAGTCCACTTGGCACGATTACCCGTTGAGCCACATATTGGAAA
AATGATTCTTTTTGGAGCACTGTTCTGCTGCTTAGACCCAGTACTCACTA
TTGCTGCTAGTCTCAGTTTCAAAGATCCATTTGTCATTCCACTGGGCTGG
GAAGAGGCTAGGCGACGTGGTTTCAGATACGAAAAGGACTATTGCTGGGA
ATATTTTCTGTCTTCAAACACACTGCAGATGCTGCATAACATGAAAGGAC
AGTTTGCTGAGCATCTTCTTGGAGCTGGATTTGTAAGCAGTAGAAATCCT
AAAGATCCAGAATCTAATATAAATTCAGATAATGAGAAGATAATTAAAGC
TGTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATTCGACTAAATT
TGGGTAAAAAAAGAAAAATGGTAAAAGTTTACACAAAAACCGATGGCCTG
GTTGCTGTTCATCCTAAATCTGTTAATGTGGAGCAAACAGACTTTCACTA
CAACTGGCTTATCTATCACCTAAAGATGAGAACAAGCAGTATATACTTGT
ATGACTGCACAGAGGTTTCCCCATACTGTCTCTTGTTTTTTGGAGGTGAC
ATTTCCATCCAGAAGGATAACGATCAGGAAACTATTGCTGTAGATGAGTG
GATTGTATTTCAGTCTCCAGCAAGAATTGCCCATCTTGTTAAGGAATTAA
GAAAGGAACTAGATATTCTTCTGCAAGAGAAGATTGAAAGTCCTCATCCT
GTAGACTGGAATGACACTAAATCCAGAGACTGTGCAGTACTGTCAGCTAT
TATAGACTTGATCAAAACACAGGAAAAGGCAACTCCCAGGAACTTTCCGC
CACGATTCCAGGATGGATATTACAGCTGAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKGSMSYDYHQNWGRDGGPRSSGGGYGGGPAGGHGGNRGSGGG
GGGGGGGRGGRGRHPGHLKGREIGMWYAKKQGQKNKEAERQERAVVHMDE
RREEQIVQLLNSVQAKNDKESEAQISWFAPEDHGYGTEVSTKNTPCSENK
LDIQEKKLINQEKKMFRIRNRSYIDRDSEYLLQENEPDGTLDQKLLEDLQ
KKKNDLRYIEMQHFREKLPSYGMQKELVNLIDNHQVTVISGETGCGKTTQ
VTQFILDNYIERGKGSACRIVCTQPRRISAISVAERVAAERAESCGSGNS
TGYQIRLQSRLPRKQGSILYCTTGIILQWLQSDPYLSSVSHIVLDEIHER
NLQSDVLMTVVKDLLNFRSDLKVILMSATLNAEKFSEYFGNCPMIHIPGF
TFPVVEYLLEDVIEKIRYVPEQKEHRCQFKRGFMQGHVNRQEKEEKEAIY
KERWPDYVRELRRRYSASTVDVIEMMEDDKVDLNLIVALIRYIVLEEEDG
AILVFLPGWDNISTLHDLLMSQVMFKSDKFLIIPLHSLMPTVNQTQVFKR
TPPGVRKIVIATNIAETSITIDDVVYVIDGGKIKETHFDTQNNNSTMSAE
WVSKANAKQRKGRAGRVQPGHCYHLYNGLRASLLDDYQLPEILRTPLEEL
CLQIKILRLGGIAYFLSRLMDPPSNEAVLLSIRHLMELNALDKQEELTPL
GVHLARLPVEPHIGKMILFGALFCCLDPVLTIAASLSFKDPFVIPLGWEE
ARRRGFRYEKDYCWEYFLSSNTLQMLHNMKGQFAEHLLGAGFVSSRNPKD
PESNINSDNEKIIKAVICAGLYPKVAKIRLNLGKKRKMVKVYTKTDGLVA
VHPKSVNVEQTDFHYNWLIYHLKMRTSSIYLYDCTEVSPYCLLFFGGDIS
IQKDNDQETIAVDEWIVFQSPARIAHLVKELRKELDILLQEKIESPHPVD
WNDTKSRDCAVLSAIIDLIKTQEKATPRNFPPRFQDGYYS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4787
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term FLAG.