cDNA Clone MLEL1

• Plasmid: pGEX6P-1-MLEL1
• Parent Plasmid: pGEX6P1
• Expressed: GST-MLEL1
• Genbank: AF217190
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCATGAGTT
ATGACTACCATCAGAACTGGGGCCGTGATGGGGGTCCCCGCAGCTCCGGT
GGGGGCTATGGAGGGGGGCCAGCAGGGGGTCATGGAGGTAACCGAGGCTC
CGGAGGAGGCGGCGGCGGCGGAGGGGGTGGTCGAGGCGGCAGGGGCCGGC
ATCCCGGGCACCTGAAAGGCCGCGAAATCGGCATGTGGTACGCGAAAAAA
CAGGGGCAGAAGAACAAGGAAGCGGAGAGGCAAGAGAGAGCTGTAGTACA
CATGGATGAACGACGAGAAGAACAAATTGTACAGTTACTGAATTCTGTTC
AAGCGAAGAATGATAAAGAGTCAGAAGCACAGATATCCTGGTTTGCTCCT
GAGGATCATGGATACGGTACTGAAGTTTCTACTAAGAACACACCATGCTC
AGAGAACAAACTTGACATCCAGGAAAAGAAGTTGATAAATCAAGAAAAAA
AAATGTTTAGAATCAGGAACAGATCATATATTGACCGAGATTCTGAGTAT
CTCTTGCAAGAAAATGAACCAGATGGAACTTTAGACCAAAAATTATTGGA
AGATTTACAAAAGAAAAAAAATGACCTTCGGTATATTGAAATGCAGCATT
TCAGAGAAAAGCTGCCTTCGTATGGAATGCAAAAGGAATTGGTAAATTTA
ATTGATAACCATCAGGTAACAGTAATAAGTGGTGAAACTGGTTGTGGCAA
AACCACTCAAGTTACTCAGTTCATTTTGGATAACTACATTGAAAGAGGAA
AAGGATCTGCTTGCAGAATAGTTTGTACTCAGCCAAGAAGAATTAGTGCC
ATTTCAGTTGCGGAAAGAGTAGCTGCAGAAAGGGCAGAATCTTGTGGCAG
TGGTAATAGTACTGGATATCAAATTCGTCTCCAGAGTCGGTTGCCAAGGA
AACAGGGTTCTATCTTATACTGTACAACAGGAATCATCCTTCAGTGGCTC
CAGTCAGACCCGTATTTGTCCAGTGTTAGTCATATCGTACTTGATGAAAT
CCATGAAAGAAATCTGCAGTCAGATGTTTTAATGACTGTTGTTAAAGACC
TTCTCAATTTTCGATCTGACTTGAAAGTAATATTGATGAGTGCAACATTG
AATGCAGAAAAGTTTTCAGAATATTTTGGTAACTGTCCAATGATACATAT
ACCTGGTTTTACCTTTCCGGTTGTGGAATATCTTTTGGAAGATGTAATTG
AAAAAATAAGGTATGTTCCAGAACAAAAAGAACACAGATGCCAGTTTAAG
AGGGGTTTCATGCAAGGGCATGTAAATAGACAAGAAAAAGAAGAAAAAGA
AGCAATATATAAAGAACGTTGGCCAGATTATGTAAGGGAACTGCGAAGAA
GGTATTCTGCAAGTACTGTAGATGTTATAGAAATGATGGAGGATGATAAA
GTTGATCTGAATTTGATTGTTGCCCTCATCCGATACATTGTTTTGGAAGA
AGAGGATGGTGCGATACTGGTCTTTCTGCCAGGCTGGGACAATATCAGCA
CTTTACATGATCTCTTGATGTCACAAGTAATGTTTAAATCAGATAAATTT
TTAATTATACCTTTACATTCACTGATGCCTACAGTTAACCAGACACAGGT
GTTTAAAAGAACCCCTCCTGGTGTTCGGAAAATAGTAATTGCTACCAACA
TTGCGGAGACTAGCATTACCATAGATGATGTCGTTTATGTGATAGATGGA
GGAAAAATAAAAGAGACGCATTTTGATACTCAGAACAATAACAGTACAAT
GTCCGCTGAGTGGGTTAGTAAAGCTAATGCCAAACAGAGAAAAGGTCGAG
CTGGAAGAGTTCAACCTGGTCATTGCTATCATCTGTATAATGGTCTTAGA
GCAAGTCTTCTAGATGACTATCAACTGCCAGAAATTTTGAGAACTCCTTT
GGAAGAACTTTGTTTACAAATAAAGATTTTAAGGCTAGGTGGAATTGCTT
ATTTTCTGAGTAGATTAATGGACCCACCATCAAATGAGGCAGTGTTACTC
TCCATAAGACACCTGATGGAGCTGAACGCTTTGGATAAACAAGAAGAATT
GACACCTCTTGGAGTCCACTTGGCACGATTACCCGTTGAGCCACATATTG
GAAAAATGATTCTTTTTGGAGCACTGTTCTGCTGCTTAGACCCAGTACTC
ACTATTGCTGCTAGTCTCAGTTTCAAAGATCCATTTGTCATTCCACTGGG
CTGGGAAGAGGCTAGGCGACGTGGTTTCAGATACGAAAAGGACTATTGCT
GGGAATATTTTCTGTCTTCAAACACACTGCAGATGCTGCATAACATGAAA
GGACAGTTTGCTGAGCATCTTCTTGGAGCTGGATTTGTAAGCAGTAGAAA
TCCTAAAGATCCAGAATCTAATATAAATTCAGATAATGAGAAGATAATTA
AAGCTGTCATCTGTGCTGGTTTATATCCCAAAGTTGCTAAAATTCGACTA
AATTTGGGTAAAAAAAGAAAAATGGTAAAAGTTTACACAAAAACCGATGG
CCTGGTTGCTGTTCATCCTAAATCTGTTAATGTGGAGCAAACAGACTTTC
ACTACAACTGGCTTATCTATCACCTAAAGATGAGAACAAGCAGTATATAC
TTGTATGACTGCACAGAGGTTTCCCCATACTGTCTCTTGTTTTTTGGAGG
TGACATTTCCATCCAGAAGGATAACGATCAGGAAACTATTGCTGTAGATG
AGTGGATTGTATTTCAGTCTCCAGCAAGAATTGCCCATCTTGTTAAGGAA
TTAAGAAAGGAACTAGATATTCTTCTGCAAGAGAAGATTGAAAGTCCTCA
TCCTGTAGACTGGAATGACACTAAATCCAGAGACTGTGCAGTACTGTCAG
CTATTATAGACTTGATCAAAACACAGGAAAAGGCAACTCCCAGGAACTTT
CCGCCACGATTCCAGGATGGATATTACAGCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMSYDYHQNWGRDGGPRSSG
GGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIGMWYAKK
QGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQISWFAP
EDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYIDRDSEY
LLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQKELVNL
IDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQPRRISA
ISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTGIILQWL
QSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVILMSATL
NAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKEHRCQFK
RGFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIEMMEDDK
VDLNLIVALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVMFKSDKF
LIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITIDDVVYVIDG
GKIKETHFDTQNNNSTMSAEWVSKANAKQRKGRAGRVQPGHCYHLYNGLR
ASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPSNEAVLL
SIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFCCLDPVL
TIAASLSFKDPFVIPLGWEEARRRGFRYEKDYCWEYFLSSNTLQMLHNMK
GQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPKVAKIRL
NLGKKRKMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKMRTSSIY
LYDCTEVSPYCLLFFGGDISIQKDNDQETIAVDEWIVFQSPARIAHLVKE
LRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEKATPRNF
PPRFQDGYYS*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4786
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.