cDNA Clone MLEL1

• Plasmid: pEBG6P-1 MLEL1
• Parent Plasmid: pEBG6P
• Expressed: GST-MLEL1
• Genbank: AF217190
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGAGTTATGACTACCATCAGAACTGGGGCCGTGATGGG
GGTCCCCGCAGCTCCGGTGGGGGCTATGGAGGGGGGCCAGCAGGGGGTCA
TGGAGGTAACCGAGGCTCCGGAGGAGGCGGCGGCGGCGGAGGGGGTGGTC
GAGGCGGCAGGGGCCGGCATCCCGGGCACCTGAAAGGCCGCGAAATCGGC
ATGTGGTACGCGAAAAAACAGGGGCAGAAGAACAAGGAAGCGGAGAGGCA
AGAGAGAGCTGTAGTACACATGGATGAACGACGAGAAGAACAAATTGTAC
AGTTACTGAATTCTGTTCAAGCGAAGAATGATAAAGAGTCAGAAGCACAG
ATATCCTGGTTTGCTCCTGAGGATCATGGATACGGTACTGAAGTTTCTAC
TAAGAACACACCATGCTCAGAGAACAAACTTGACATCCAGGAAAAGAAGT
TGATAAATCAAGAAAAAAAAATGTTTAGAATCAGGAACAGATCATATATT
GACCGAGATTCTGAGTATCTCTTGCAAGAAAATGAACCAGATGGAACTTT
AGACCAAAAATTATTGGAAGATTTACAAAAGAAAAAAAATGACCTTCGGT
ATATTGAAATGCAGCATTTCAGAGAAAAGCTGCCTTCGTATGGAATGCAA
AAGGAATTGGTAAATTTAATTGATAACCATCAGGTAACAGTAATAAGTGG
TGAAACTGGTTGTGGCAAAACCACTCAAGTTACTCAGTTCATTTTGGATA
ACTACATTGAAAGAGGAAAAGGATCTGCTTGCAGAATAGTTTGTACTCAG
CCAAGAAGAATTAGTGCCATTTCAGTTGCGGAAAGAGTAGCTGCAGAAAG
GGCAGAATCTTGTGGCAGTGGTAATAGTACTGGATATCAAATTCGTCTCC
AGAGTCGGTTGCCAAGGAAACAGGGTTCTATCTTATACTGTACAACAGGA
ATCATCCTTCAGTGGCTCCAGTCAGACCCGTATTTGTCCAGTGTTAGTCA
TATCGTACTTGATGAAATCCATGAAAGAAATCTGCAGTCAGATGTTTTAA
TGACTGTTGTTAAAGACCTTCTCAATTTTCGATCTGACTTGAAAGTAATA
TTGATGAGTGCAACATTGAATGCAGAAAAGTTTTCAGAATATTTTGGTAA
CTGTCCAATGATACATATACCTGGTTTTACCTTTCCGGTTGTGGAATATC
TTTTGGAAGATGTAATTGAAAAAATAAGGTATGTTCCAGAACAAAAAGAA
CACAGATGCCAGTTTAAGAGGGGTTTCATGCAAGGGCATGTAAATAGACA
AGAAAAAGAAGAAAAAGAAGCAATATATAAAGAACGTTGGCCAGATTATG
TAAGGGAACTGCGAAGAAGGTATTCTGCAAGTACTGTAGATGTTATAGAA
ATGATGGAGGATGATAAAGTTGATCTGAATTTGATTGTTGCCCTCATCCG
ATACATTGTTTTGGAAGAAGAGGATGGTGCGATACTGGTCTTTCTGCCAG
GCTGGGACAATATCAGCACTTTACATGATCTCTTGATGTCACAAGTAATG
TTTAAATCAGATAAATTTTTAATTATACCTTTACATTCACTGATGCCTAC
AGTTAACCAGACACAGGTGTTTAAAAGAACCCCTCCTGGTGTTCGGAAAA
TAGTAATTGCTACCAACATTGCGGAGACTAGCATTACCATAGATGATGTC
GTTTATGTGATAGATGGAGGAAAAATAAAAGAGACGCATTTTGATACTCA
GAACAATAACAGTACAATGTCCGCTGAGTGGGTTAGTAAAGCTAATGCCA
AACAGAGAAAAGGTCGAGCTGGAAGAGTTCAACCTGGTCATTGCTATCAT
CTGTATAATGGTCTTAGAGCAAGTCTTCTAGATGACTATCAACTGCCAGA
AATTTTGAGAACTCCTTTGGAAGAACTTTGTTTACAAATAAAGATTTTAA
GGCTAGGTGGAATTGCTTATTTTCTGAGTAGATTAATGGACCCACCATCA
AATGAGGCAGTGTTACTCTCCATAAGACACCTGATGGAGCTGAACGCTTT
GGATAAACAAGAAGAATTGACACCTCTTGGAGTCCACTTGGCACGATTAC
CCGTTGAGCCACATATTGGAAAAATGATTCTTTTTGGAGCACTGTTCTGC
TGCTTAGACCCAGTACTCACTATTGCTGCTAGTCTCAGTTTCAAAGATCC
ATTTGTCATTCCACTGGGCTGGGAAGAGGCTAGGCGACGTGGTTTCAGAT
ACGAAAAGGACTATTGCTGGGAATATTTTCTGTCTTCAAACACACTGCAG
ATGCTGCATAACATGAAAGGACAGTTTGCTGAGCATCTTCTTGGAGCTGG
ATTTGTAAGCAGTAGAAATCCTAAAGATCCAGAATCTAATATAAATTCAG
ATAATGAGAAGATAATTAAAGCTGTCATCTGTGCTGGTTTATATCCCAAA
GTTGCTAAAATTCGACTAAATTTGGGTAAAAAAAGAAAAATGGTAAAAGT
TTACACAAAAACCGATGGCCTGGTTGCTGTTCATCCTAAATCTGTTAATG
TGGAGCAAACAGACTTTCACTACAACTGGCTTATCTATCACCTAAAGATG
AGAACAAGCAGTATATACTTGTATGACTGCACAGAGGTTTCCCCATACTG
TCTCTTGTTTTTTGGAGGTGACATTTCCATCCAGAAGGATAACGATCAGG
AAACTATTGCTGTAGATGAGTGGATTGTATTTCAGTCTCCAGCAAGAATT
GCCCATCTTGTTAAGGAATTAAGAAAGGAACTAGATATTCTTCTGCAAGA
GAAGATTGAAAGTCCTCATCCTGTAGACTGGAATGACACTAAATCCAGAG
ACTGTGCAGTACTGTCAGCTATTATAGACTTGATCAAAACACAGGAAAAG
GCAACTCCCAGGAACTTTCCGCCACGATTCCAGGATGGATATTACAGCTG
AGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMSYDYHQNWGRDG
GPRSSGGGYGGGPAGGHGGNRGSGGGGGGGGGGRGGRGRHPGHLKGREIG
MWYAKKQGQKNKEAERQERAVVHMDERREEQIVQLLNSVQAKNDKESEAQ
ISWFAPEDHGYGTEVSTKNTPCSENKLDIQEKKLINQEKKMFRIRNRSYI
DRDSEYLLQENEPDGTLDQKLLEDLQKKKNDLRYIEMQHFREKLPSYGMQ
KELVNLIDNHQVTVISGETGCGKTTQVTQFILDNYIERGKGSACRIVCTQ
PRRISAISVAERVAAERAESCGSGNSTGYQIRLQSRLPRKQGSILYCTTG
IILQWLQSDPYLSSVSHIVLDEIHERNLQSDVLMTVVKDLLNFRSDLKVI
LMSATLNAEKFSEYFGNCPMIHIPGFTFPVVEYLLEDVIEKIRYVPEQKE
HRCQFKRGFMQGHVNRQEKEEKEAIYKERWPDYVRELRRRYSASTVDVIE
MMEDDKVDLNLIVALIRYIVLEEEDGAILVFLPGWDNISTLHDLLMSQVM
FKSDKFLIIPLHSLMPTVNQTQVFKRTPPGVRKIVIATNIAETSITIDDV
VYVIDGGKIKETHFDTQNNNSTMSAEWVSKANAKQRKGRAGRVQPGHCYH
LYNGLRASLLDDYQLPEILRTPLEELCLQIKILRLGGIAYFLSRLMDPPS
NEAVLLSIRHLMELNALDKQEELTPLGVHLARLPVEPHIGKMILFGALFC
CLDPVLTIAASLSFKDPFVIPLGWEEARRRGFRYEKDYCWEYFLSSNTLQ
MLHNMKGQFAEHLLGAGFVSSRNPKDPESNINSDNEKIIKAVICAGLYPK
VAKIRLNLGKKRKMVKVYTKTDGLVAVHPKSVNVEQTDFHYNWLIYHLKM
RTSSIYLYDCTEVSPYCLLFFGGDISIQKDNDQETIAVDEWIVFQSPARI
AHLVKELRKELDILLQEKIESPHPVDWNDTKSRDCAVLSAIIDLIKTQEK
ATPRNFPPRFQDGYYS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4780
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.