cDNA Clone NCC

• Plasmid: pHR-Lenti FLAG SLC12A3 (hNCC) T46A T50A T55A
• Parent Plasmid: pHR-Lenti
• Expressed: FLAG-SLC12A3 (hNCC) T46A T50A T55A
• Genbank: P55017
• Tag: FLAG
• Species: Human

Sequence of Insert

CCCCATATCGGGGCGCGGCAGTCATCCGACAGACTGAGTCGCCCGGGGGG
GATCCGCCACCATGGACTACAAGGACGACGATGACAAGATGGCAGAACTG
CCCACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGCTTCAC
CATCAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCCTATG
ACAGCAGCCACCCCAGCCACCTGGCCCACAGCAGCGCCTTCTGCATGCGC
GCCTTTGGCTATAACACGATCGATGTGGTGCCCACATATGAGCACTATGC
CAACAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACTGGCTG
ACCTGCACTCCTTCCTCAAGCAGGAAGGCAGACACCTGCATGCCCTGGCC
TTTGACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAGGGCGA
GGCAGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTTCGGCT
GGGTCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGGGGCGTGATC
CTCTACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGGCATCGTCCTGAC
CTGGATCATCATCCTGCTGTCGGTCACGGTGACCTCCATCACAGGCCTCT
CCATCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGTGGCACCTAC
TTCCTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCGGCCT
CATTTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGCACACGGTGGGCT
TTGCAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCACCCATCGTG
GACCCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGTCACTGTGCT
GCTGGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAGGCCCAGGTGC
TGTTCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTAGTGGGGACG
CTGATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCTACCG
GGCGGACATTTTTGTCCAAAACTTGGTGCCTGACTGGCGGGGTCCAGATG
GCACCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCCACAGGCATC
CTGGCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGCTATAGCCAT
CCCCAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTACCTGG
CCATCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGATGCCTCTGGGGTC
CTGAATGACACAGTGACCCCTGGCTGGGGTGCCTGCGAGGGGCTGGCCTG
CAGCTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGCCACT
ACGGCCTCATCAACTATTACCAGACCATGAGCATGGTGTCAGGCTTCGCG
CCCCTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTCTGCCCTGGC
CTGCCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTTGCGAGGACCAGC
TGTACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAGAACAAGGAG
CCCGTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCATCAT
CATCGCTGAGCTCAACACCATAGCCCCCATCATTTCCAACTTCTTCCTCT
GCTCCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATCACCAAC
TCGCCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGGCGCT
GTTTGGGGCTATCATCTCCGTGGTCATCATGTTCCTCCTCACCTGGTGGG
CGGCCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTGCTCTATGTCATC
TACAAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGCTCCTA
CAACCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGACCACA
TCAAGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGGCCCCCCAACTTC
CGCCCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGGAACCTCAGCCT
GATGATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGAGGATGCCTG
AGCTCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAGGAAG
ATCAAGGCCTTCTACTCGGATGTCATTGCTGAGGACCTCCGCAGAGGCGT
CCAGATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAACATTC
TGGTGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCACAGTG
GAAGACTACATTGGCATCCTCCATGATGCCTTTGATTTCAACTATGGCGT
GTGTGTCATGAGGATGCGGGAGGGACTCAACGTGTCCAAGATGATGCAGG
CGCACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGCCAGC
GCCAGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACCACCAT
CTTCCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTCTTTG
ACGATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTTGGCCGCAAGAGG
AGGTGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGGCCAGATTAACAG
GATGGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCAAGTTCCGGC
TGGGATTCCATGAAGTCCACATCCTCCCTGACATCAACCAGAACCCTCGG
GCTGAGCACACCAAGAGGTTTGAGGACATGATTGCACCCTTCCGTCTGAA
TGATGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGGCGGGACTGCC
CCTGGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGTCCCTT
CGGCAGGTGAGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACGCTGC
TCTCATCGTCATCACTTTGCCCATAGGGAGGAAGGGGAAGTGCCCCAGCT
CGCTGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTCAGACCTCCA
GTCATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTTTTACTGCCA
GTAAGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSH
PSHLAHSSAFCMRAFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHS
FLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKG
VMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISA
ISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAET
VRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFL
VIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFF
GMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISA
TIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLI
NYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPL
IGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYA
LINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALI
AIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNY
RPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQL
IANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVG
FKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHIN
PVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGG
LTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFH
EVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKI
SDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYM
AWLETLSQDLRPPVILIRGNQENVLTFYCQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4657
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal FLAG.