cDNA Clone NCC

• Plasmid: pHR-Lenti FLAG SLC12A3 (hNCC)
• Parent Plasmid: pHR-Lenti
• Expressed: FLAG-SLC12A3 (hNCC)
• Genbank: P55017
• Tag: FLAG
• Species: Human

Sequence of Insert

CCCCCTCTCGGCGCGCAGTCATCCGACAGACTGAGTCGCCCGGGGGGGAT
CCGCCACCATGGACTACAAGGACGACGATGACAAGATGGCAGAACTGCCC
ACAACAGAGACGCCTGGGGACGCCACTTTGTGCAGCGGGCGCTTCACCAT
CAGCACACTGCTGAGCAGTGATGAGCCCTCTCCACCAGCTGCCTATGACA
GCAGCCACCCCAGCCACCTGACCCACAGCAGCACCTTCTGCATGCGCACC
TTTGGCTACAACACGATCGATGTGGTGCCCACATATGAGCACTATGCCAA
CAGCACCCAGCCTGGTGAGCCCCGGAAGGTCCGGCCCACACTGGCTGACC
TGCACTCCTTCCTCAAGCAGGAAGGCAGACACCTGCATGCCCTGGCCTTT
GACAGCCGGCCCAGCCACGAGATGACTGATGGGCTGGTGGAGGGCGAGGC
AGGCACCAGCAGCGAGAAGAACCCCGAGGAGCCAGTGCGCTTCGGCTGGG
TCAAGGGGGTGATGATTCGTTGCATGCTCAACATTTGGGGCGTGATCCTC
TACCTGCGGCTGCCCTGGATTACGGCCCAGGCAGGCATCGTCCTGACCTG
GATCATCATCCTGCTGTCGGTCACGGTGACCTCCATCACAGGCCTCTCCA
TCTCAGCCATCTCCACCAATGGCAAGGTCAAGTCAGGTGGCACCTACTTC
CTCATCTCCCGGAGTCTGGGCCCAGAGCTTGGGGGCTCCATCGGCCTCAT
TTTCGCTTTCGCCAATGCCGTGGGTGTGGCCATGCACACGGTGGGCTTTG
CAGAGACCGTGCGGGACCTGCTCCAGGAGTATGGGGCACCCATCGTGGAC
CCCATTAACGACATCCGCATCATTGGCGTGGTCTCGGTCACTGTGCTGCT
GGCCATCTCCCTGGCTGGCATGGAGTGGGAGTCCAAGGCCCAGGTGCTGT
TCTTCCTTGTCATCATGGTCTCCTTTGCCAACTATTTAGTGGGGACGCTG
ATCCCCCCATCTGAGGACAAGGCCTCCAAAGGCTTCTTCAGCTACCGGGC
GGACATTTTTGTCCAAAACTTGGTGCCTGACTGGCGGGGTCCAGATGGCA
CCTTCTTCGGAATGTTCTCCATCTTCTTCCCCTCGGCCACAGGCATCCTG
GCAGGGGCCAACATATCTGGTGACCTCAAGGACCCTGCTATAGCCATCCC
CAAGGGGACCCTCATGGCCATTTTCTGGACGACCATTTCCTACCTGGCCA
TCTCAGCCACCATTGGCTCCTGCGTGGTGCGTGATGCCTCTGGGGTCCTG
AATGACACAGTGACCCCTGGCTGGGGTGCCTGCGAGGGGCTGGCCTGCAG
CTATGGCTGGAACTTCACCGAGTGCACCCAGCAGCACAGCTGCCACTACG
GCCTCATCAACTATTACCAGACCATGAGCATGGTGTCAGGCTTCGCGCCC
CTGATCACGGCTGGCATCTTCGGGGCCACCCTCTCCTCTGCCCTGGCCTG
CCTTGTCTCTGCTGCCAAAGTCTTCCAGTGCCTTTGCGAGGACCAGCTGT
ACCCACTGATCGGCTTCTTCGGCAAAGGCTATGGCAAGAACAAGGAGCCC
GTGCGTGGCTACCTGCTGGCCTACGCCATCGCTGTGGCCTTCATCATCAT
CGCTGAGCTCAACACCATAGCCCCCATCATTTCCAACTTCTTCCTCTGCT
CCTATGCCCTCATCAACTTCAGCTGCTTCCACGCCTCCATCACCAACTCG
CCTGGGTGGAGACCTTCATTCCAATACTACAACAAGTGGGCGGCGCTGTT
TGGGGCTATCATCTCCGTGGTCATCATGTTCCTCCTCACCTGGTGGGCGG
CCCTCATCGCCATTGGCGTGGTGCTCTTCCTCCTGCTCTATGTCATCTAC
AAGAAGCCAGAGGTAAATTGGGGCTCCTCGGTACAGGCTGGCTCCTACAA
CCTGGCCCTCAGCTACTCGGTGGGCCTCAATGAGGTGGAAGACCACATCA
AGAACTACCGCCCCCAGTGCCTGGTGCTCACGGGGCCCCCCAACTTCCGC
CCGGCCCTGGTGGACTTTGTGGGCACCTTCACCCGGAACCTCAGCCTGAT
GATCTGTGGCCACGTGCTCATCGGACCCCACAAGCAGAGGATGCCTGAGC
TCCAGCTCATCGCCAACGGGCACACCAAGTGGCTGAACAAGAGGAAGATC
AAGGCCTTCTACTCGGATGTCATTGCTGAGGACCTCCGCAGAGGCGTCCA
GATCCTCATGCAGGCCGCAGGTCTCGGGAGAATGAAGCCCAACATTCTGG
TGGTTGGGTTCAAGAAGAACTGGCAGTCGGCTCACCCGGCCACAGTGGAA
GACTACATTGGCATCCTCCATGATGCCTTTGATTTCAACTATGGCGTGTG
TGTCATGAGGATGCGGGAGGGACTCAACGTGTCCAAGATGATGCAGGCGC
ACATTAACCCCGTGTTTGACCCAGCGGAGGACGGGAAGGAAGCCAGCGCC
AGAGTGGACCCCAAGGCCCTGGTGAAGGAGGAGCAGGCCACCACCATCTT
CCAGTCGGAGCAGGGCAAGAAGACCATAGACATCTACTGGCTCTTTGACG
ATGGAGGCCTCACCCTCCTCATTCCCTATCTCCTTGGCCGCAAGAGGAGG
TGGAGCAAATGCAAGATCCGTGTGTTCGTAGGTGGCCAGATTAACAGGAT
GGACCAGGAGAGAAAGGCGATCATTTCTCTGCTGAGCAAGTTCCGACTGG
GATTCCATGAAGTCCACATCCTCCCTGACATCAACCAGAACCCTCGGGCT
GAGCACACCAAGAGGTTTGAGGACATGATTGCACCCTTCCGTCTGAATGA
TGGCTTCAAGGATGAGGCCACTGTCAACGAGATGCGGCGGGACTGCCCCT
GGAAGATCTCAGATGAGGAGATTACGAAGAACAGAGTCAAGTCCCTTCGG
CAGGTGAGGCTGAATGAGATTGTGCTGGATTACTCCCGAGACGCTGCTCT
CATCGTCATCACTTTGCCCATAGGGAGGAAGGGGAAGTGCCCCAGCTCGC
TGTACATGGCCTGGCTGGAGACCCTGTCCCAGGACCTCAGACCTCCAGTC
ATCCTGATCCGAGGAAACCAGGAAAACGTGCTCACCTTTTACTGCCAGTA
AGCGGCCGCG

Amino Acid Sequence

MDYKDDDDKMAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSH
PSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHS
FLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKG
VMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISA
ISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAET
VRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFL
VIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFF
GMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISA
TIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLI
NYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPL
IGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYA
LINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALI
AIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNY
RPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQL
IANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVG
FKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHIN
PVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGG
LTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFH
EVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKI
SDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYM
AWLETLSQDLRPPVILIRGNQENVLTFYCQ*AAA

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4656
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal FLAG.