cDNA Clone hn RNP U

• Plasmid: pFastbac HTa MEKK3 FL S526D
• Parent Plasmid: pSC-A
• Expressed: His-MEKK3 FL S526D
• Genbank: U78876
• Tag: 6His
• Species: Human

Sequence of Insert

ATGTCGTACTACCATCACCATCACCATCACGATTACGATATCCCAACGAC
CGAAAACCTGTATTTTCAGGGCGCCATGGATCCGGAATTCGACGAACAGG
AGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTCCAGATGAACCGA
CGTCACCGGATGCCTGGATATGAGACCATGAAGAACAAAGACACAGGTCA
CTCAAATAGGCAGAGTGACGTCAGAATCAAGTTCGAGCACAACGGGGAGA
GGCGAATTATAGCGTTCAGCCGGCCTGTGAAATATGAAGATGTGGAGCAC
AAGGTGACAACAGTATTTGGACAACCTCTTGATCTACATTACATGAACAA
TGAGCTCTCCATCCTGCTGAAAAACCAAGATGATCTTGATAAAGCAATTG
ACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGCTGTTG
TCCCAGGACAGAAACCATAACAGTTCCTCTCCCCACTCTGAGGTGTCCAG
ACAGGTGCGGATCAAGGCTTCCCAGTCCGCAGGGGATATAAATACTATCT
ACCAGCCCCCCGAGCCCAGAAGCAGGCACCTCTCTGTCAGCTCCCAGAAC
CCTGGCCGAAGCTCACCTCCCCCTGGCTATGTTCCTGAGCGGCAGCAGCA
CATTGCCCGGCAGGGGTCCTACACCAGCATCAACAGTGAGGGGGAGTTCA
TCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTGAGCAGTGCAGAA
AATTCCTTGTCTGGAAGCTGCCAATCCTTGGACAGGTCAGCAGACAGCCC
ATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCTGACAACA
GACAGGAATACTCAGATCGGGAAACTCAGCTTTATGACAAAGGGGTCAAA
GGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCACCACAAGGACTA
CAGTGATGGCAGAAGAACATTTCCCCGAATACGGCGTCATCAAGGCAACT
TGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGGCGAGAAC
ATGGGCCTGGCTGTGCAATACCTGGACCCCCGTGGGCGCCTGCGGAGTGC
GGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGT
CTCCCAGTGCCCCCATCAACTGGCGCCGGGGAAAGCTCCTGGGCCAGGGT
GCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACACGGGACGTGAACT
TGCTTCCAAGCAGGTCCAATTTGATCCAGACAGTCCTGAGACAAGCAAGG
AGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAGCAT
GAGCGCATCGTGCAGTACTATGGCTGTCTGCGGGACCGCGCTGAGAAGAC
CCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGT
TGAAGGCTTACGGTGCTCTGACAGAGAGCGTGACCCGAAAGTACACGCGG
CAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACATGATTGTTCACCG
GGACATTAAGGGAGCCAACATCCTCCGAGACTCTGCTGGGAATGTAAAGC
TGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTATGTCGGGG
ACGGGCATGCGCGACGTCACTGGCACACCCTACTGGATGAGCCCTGAGGT
GATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCT
GCACTGTGGTGGAGATGCTGACAGAGAAACCACCGTGGGCAGAGTATGAA
GCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCACCAATCCTCAGCT
GCCCTCCCACATCTCTGAACATGGCCGGGACTTCCTGAGGCGCATTTTTG
TGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACACCACTTT
GCACAGCTCATGTACTGAGAATT

Amino Acid Sequence

MSYYHHHHHHDYDIPTTENLYFQGAMDPEFDEQEALNSIMNDLVALQMNR
RHRMPGYETMKNKDTGHSNRQSDVRIKFEHNGERRIIAFSRPVKYEDVEH
KVTTVFGQPLDLHYMNNELSILLKNQDDLDKAIDILDRSSSMKSLRILLL
SQDRNHNSSSPHSEVSRQVRIKASQSAGDINTIYQPPEPRSRHLSVSSQN
PGRSSPPPGYVPERQQHIARQGSYTSINSEGEFIPETSEQCMLDPLSSAE
NSLSGSCQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRETQLYDKGVK
GGTYPRRYHVSVHHKDYSDGRRTFPRIRRHQGNLFTLVPSSRSLSTNGEN
MGLAVQYLDPRGRLRSADSENALSVQERNVPTKSPSAPINWRRGKLLGQG
AFGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLKNLQH
ERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTR
QILEGMSYLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSG
TGMRDVTGTPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTEKPPWAEYE
AMAAIFKIATQPTNPQLPSHISEHGRDFLRRIFVEARQRPSAEELLTHHF
AQLMY*E

• Vector Type: Baculovirus
• Antibiotic: Amp
• DU Number: DU4584
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Baculovirus expression with N term 6H tag.