cDNA Clone JAK1

• Plasmid: pEBG6P-1 JAK1
• Parent Plasmid: pEBG6P
• Expressed: GST-JAK1
• Genbank: NM_002227
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAG
AAGACTGAGGTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTT
CTATCTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAG
CAGAGGAACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTT
TGTCACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGC
TCCAAATCGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACT
ACCGGATGAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAG
CAGTCAGTGTGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTATGAGAA
AAAAAAGATTCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGT
ATCTGTTTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATT
CGAGACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCT
AGGGATGGCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGC
AGTTGCCAGAACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAA
ACATTGAATAAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGAT
AAATAATGTTTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTT
GTGACAGCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACC
TTGGAAACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCAT
GTTACTGATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACG
GTGGAAACGTTCTCTACTACGAAGTGATGGTGACTGGGAATCTTGGAATC
CAGTGGAGGCATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAA
ACTGAAGCGGAAAAAACTGGAAAATAAAGACAAGAAGGATGAGGAGAAAA
ACAAGATCCGGGAAGAGTGGAACAATTTTTCATTCTTCCCTGAAATCACT
CACATTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAA
GAAAATGGAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGT
CCCTGGTAGATGGCTACTTCCGGCTCACAGCAGATGCCCATCATTACCTC
TGCACCGACGTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTG
TCATGGTCCAATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAG
GAAGCGAGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGAC
AACATCCTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGG
TGCCCAGAAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCT
ACAGTCTGCACGGTTCGGACCGCAGCTTCCCCAGCTTGGGAGACCTCATG
AGCCACCTCAAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCT
AAAACGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGG
CTACTAAGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTG
AGTTTCGATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGG
GAGAGGCACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGG
ATGACGAAGGAACTTCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTC
TTAGACCCCAGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAG
CATGATGAGACAGGTCTCCCACAAGCACATCGTGTACCTCTATGGCGTCT
GTGTCCGCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGT
CCTCTGGATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATG
GAAATTCAAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGG
ATAAAGACCTGGTCCATGGAAATGTGTGTACTAAAAACCTCCTCCTGGCC
CGTGAGGGCATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCC
CGGCATCCCCATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCC
CATGGATTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCT
GCTGACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGG
CGAGATCCCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATG
AAAGCCGGTGCAGGCCAGTGACACCATCATGTAAGGAGCTGGCTGACCTC
ATGACCCGCTGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGC
CATCATGAGAGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTT
CCAGAAAAAAAAACCAGCCAACTGAAGTGGACCCCACACATTTTGAAAAG
CGCTTCCTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGT
TGAGCTCTGCAGGTATGACCCCGAAGACAATACAGGGGAGCAGGTGGCTG
TTAAATCTCTGAAGCCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAA
AAGGAAATCGAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTA
CAAAGGAATCTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGG
AATTTCTGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAAC
AAAATAAACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGG
GATGGACTATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAA
GAAATGTCCTTGTTGAGAGTGAACACCAAGTGAAAATTGGAGACTTCGGT
TTAACCAAAGCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGA
CCGGGACAGCCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTA
AATTTTATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAG
CTGCTGACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAA
AATGATAGGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATA
CGTTAAAAGAAGGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAG
GTTTATCAACTTATGAGGAAATGCTGGGAATTCCAACCATCCAATCGGAC
AAGCTTTCAGAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAAGCGG
CCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSMAFCAKMRSSK
KTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPL
CHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNE
QSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPI
RDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPE
TLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLAT
LETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGI
QWRHKPNVVSVEKEKNKLKRKKLENKDKKDEEKNKIREEWNNFSFFPEIT
HIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYL
CTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFD
NILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLM
SHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQL
SFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKV
LDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGG
PLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLA
REGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVA
ADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADL
MTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSRKKNQPTEVDPTHFEK
RFLKRIRDLGEGHFGKVELCRYDPEDNTGEQVAVKSLKPESGGNHIADLK
KEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKN
KINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFG
LTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHE
LLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDE
VYQLMRKCWEFQPSNRTSFQNLIEGFEALLK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4556
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.