cDNA Clone JAK1

• Plasmid: pCMV5-HA-3 JAK1
• Parent Plasmid: pCMV5
• Expressed: HA-JAK1
• Genbank: NM_002227
• Tag: HA
• Species: Human

Sequence of Insert

GCCACCATGTACCCATACGATGTGCCAGATTACGCCGGGGATCCCCGGAA
TTCCCGGGTCGACATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGA
CTGAGGTGAACCTGGAGGCCCCTGAGCCAGGGGTGGAAGTGATCTTCTAT
CTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGTGGAGAGTACACAGCAGA
GGAACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCTCTTTGTC
ACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCA
AATCGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCG
GATGAGGTTCTATTTCACCAATTGGCATGGAACCAACGACAATGAGCAGT
CAGTGTGGCGTCATTCTCCAAAGAAGCAGAAAAATGGCTACGAGAAAAAA
AAGATTCCAGATGCAACCCCTCTCCTTGATGCCAGCTCACTGGAGTATCT
GTTTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCTATTCGAG
ACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGG
ATGGCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTT
GCCAGAACTGCCCAAGGACATCAGCTACAAGCGATATATTCCAGAAACAT
TGAATAAGTCCATCAGACAGAGGAACCTTCTCACCAGGATGCGGATAAAT
AATGTTTTCAAGGATTTCCTAAAGGAATTTAACAACAAGACCATTTGTGA
CAGCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCTACCTTGG
AAACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTA
CTGATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGG
AAACGTTCTCTACTACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGT
GGAGGCATAAACCAAATGTTGTTTCTGTTGAAAAGGAAAAAAATAAACTG
AAGCGGAAAAAACTGGAAAATAAAGACAAGAAGGATGAGGAGAAAAACAA
GATCCGGGAAGAGTGGAACAATTTTTCATTCTTCCCTGAAATCACTCACA
TTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAA
ATGGAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCT
GGTAGATGGCTACTTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCA
CCGACGTGGCCCCCCCGTTGATCGTCCACAACATACAGAATGGCTGTCAT
GGTCCAATCTGTACAGAATACGCCATCAATAAATTGCGGCAAGAAGGAAG
CGAGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTTGACAACA
TCCTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCC
CAGAAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAG
TCTGCACGGTTCGGACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCC
ACCTCAAGAAGCAGATCCTGCGCACGGATAACATCAGCTTCATGCTAAAA
CGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCCAACCTGCTGGTGGCTAC
TAAGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAGCTGAGTT
TCGATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGA
GGCACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGA
CGAAGGAACTTCTGAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAG
ACCCCAGCCACAGGGATATTTCCCTGGCCTTCTTCGAGGCAGCCAGCATG
ATGAGACAGGTCTCCCACAAACACATCGTGTACCTCTATGGCGTCTGTGT
CCGCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGGGGTCCTC
TGGATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAA
TTCAAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAA
AGACCTGGTCCATGGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTG
AGGGCATCGACAGTGAGTGTGGCCCATTCATCAAGCTCAGTGACCCCGGC
ATCCCCATTACGGTGCTGTCTAGGCAAGAATGCATTGAACGAATCCCATG
GATTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTGGCTGCTG
ACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAG
ATCCCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAG
CCGGTGCAGGCCAGTGACACCATCATGTAAGGAGCTGGCTGACCTCATGA
CCCGCTGCATGAACTATGACCCCAATCAGAGGCCTTTCTTCCGAGCCATC
ATGAGAGACATTAATAAGCTTGAAGAGCAGAATCCAGATATTGTTTCCAG
AAAAAAAAACCAGCCAACTGAAGTGGACCCCACACATTTTGAAAAGCGCT
TCCTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAG
CTCTGCAGGTATGACCCCGAAGACAATACAGGGGAGCAGGTGGCTGTTAA
ATCTCTGAAGCCTGAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGG
AAATCGAGATCTTAAGGAACCTCTATCATGAGAACATTGTGAAGTACAAA
GGAATCTGCACAGAAGACGGAGGAAATGGTATTAAGCTCATCATGGAATT
TCTGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAGAACAAAA
TAAACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATG
GACTATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAA
TGTCCTTGTTGAGAGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAA
CCAAAGCAATTGAAACCGATAAGGAGTATTACACCGTCAAGGATGACCGG
GACAGCCCTGTGTTTTGGTATGCTCCAGAATGTTTAATGCAATCTAAATT
TTATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCATGAGCTGC
TGACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATG
ATAGGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTT
AAAAGAAGGAAAACGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTT
ATCAACTTATGAGGAAATGCTGGGAATTCCAACCATCCAATCGGACAAGC
TTTCAGAACCTTATTGAAGGATTTGAAGCACTTTTAAAATAAGCGGCCGC
G

Amino Acid Sequence

MYPYDVPDYAGDPRNSRVDMAFCAKMRSSKKTEVNLEAPEPGVEVIFY
LSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAP
NRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKK
KIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLG
MAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRIN
NVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSML
LISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKL
KRKKLENKDKKDEEKNKIREEWNNFSFFPEITHIVIKESVVSINKQDNKK
MELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCH
GPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGA
QKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLK
RCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGR
GTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASM
MRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWK
FKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPG
IPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGE
IPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAI
MRDINKLEEQNPDIVSRKKNQPTEVDPTHFEKRFLKRIRDLGEGHFGKVE
LCRYDPEDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYK
GICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGM
DYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDR
DSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKM
IGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTS
FQNLIEGFEALLK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4528
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term HA.