cDNA Clone NCC

• Plasmid: pEBG6P-1 SLC12A3 (hNCC)
• Parent Plasmid: pEBG6P
• Expressed: GST-SLC12A3 (hNCC)
• Genbank: P55017
• Tag: GST

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCATGGCAGAACTGCCCACAACAGAGACGCCTGGGGACGCC
ACTTTGTGCAGCGGGCGCTTCACCATCAGCACACTGCTGAGCAGTGATGA
GCCCTCTCCACCAGCTGCCTATGACAGCAGCCACCCCAGCCACCTGACCC
ACAGCAGCACCTTCTGCATGCGCACCTTTGGCTACAACACGATCGATGTG
GTGCCCACATATGAGCACTATGCCAACAGCACCCAGCCTGGTGAGCCCCG
GAAGGTCCGGCCCACACTGGCTGACCTGCACTCCTTCCTCAAGCAGGAAG
GCAGACACCTGCATGCCCTGGCCTTTGACAGCCGGCCCAGCCACGAGATG
ACTGATGGGCTGGTGGAGGGCGAGGCAGGCACCAGCAGCGAGAAGAACCC
CGAGGAGCCAGTGCGCTTCGGCTGGGTCAAGGGGGTGATGATTCGTTGCA
TGCTCAACATTTGGGGCGTGATCCTCTACCTGCGGCTGCCCTGGATTACG
GCCCAGGCAGGCATCGTCCTGACCTGGATCATCATCCTGCTGTCGGTCAC
GGTGACCTCCATCACAGGCCTCTCCATCTCAGCCATCTCCACCAATGGCA
AGGTCAAGTCAGGTGGCACCTACTTCCTCATCTCCCGGAGTCTGGGCCCA
GAGCTTGGGGGCTCCATCGGCCTCATTTTCGCTTTCGCCAATGCCGTGGG
TGTGGCCATGCACACGGTGGGCTTTGCAGAGACCGTGCGGGACCTGCTCC
AGGAGTATGGGGCACCCATCGTGGACCCCATTAACGACATCCGCATCATT
GGCGTGGTCTCGGTCACTGTGCTGCTGGCCATCTCCCTGGCTGGCATGGA
GTGGGAGTCCAAGGCCCAGGTGCTGTTCTTCCTTGTCATCATGGTCTCCT
TTGCCAACTATTTAGTGGGGACGCTGATCCCCCCATCTGAGGACAAGGCC
TCCAAAGGCTTCTTCAGCTACCGGGCGGACATTTTTGTCCAAAACTTGGT
GCCTGACTGGCGGGGTCCAGATGGCACCTTCTTCGGAATGTTCTCCATCT
TCTTCCCCTCGGCCACAGGCATCCTGGCAGGGGCCAACATATCTGGTGAC
CTCAAGGACCCTGCTATAGCCATCCCCAAGGGGACCCTCATGGCCATTTT
CTGGACGACCATTTCCTACCTGGCCATCTCAGCCACCATTGGCTCCTGCG
TGGTGCGTGATGCCTCTGGGGTCCTGAATGACACAGTGACCCCTGGCTGG
GGTGCCTGCGAGGGGCTGGCCTGCAGCTATGGCTGGAACTTCACCGAGTG
CACCCAGCAGCACAGCTGCCACTACGGCCTCATCAACTATTACCAGACCA
TGAGCATGGTGTCAGGCTTCGCGCCCCTGATCACGGCTGGCATCTTCGGG
GCCACCCTCTCCTCTGCCCTGGCCTGCCTTGTCTCTGCTGCCAAAGTCTT
CCAGTGCCTTTGCGAGGACCAGCTGTACCCACTGATCGGCTTCTTCGGCA
AAGGCTATGGCAAGAACAAGGAGCCCGTGCGTGGCTACCTGCTGGCCTAC
GCCATCGCTGTGGCCTTCATCATCATCGCTGAGCTCAACACCATAGCCCC
CATCATTTCCAACTTCTTCCTCTGCTCCTATGCCCTCATCAACTTCAGCT
GCTTCCACGCCTCCATCACCAACTCGCCTGGGTGGAGACCTTCATTCCAA
TACTACAACAAGTGGGCGGCGCTGTTTGGGGCTATCATCTCCGTGGTCAT
CATGTTCCTCCTCACCTGGTGGGCGGCCCTCATCGCCATTGGCGTGGTGC
TCTTCCTCCTGCTCTATGTCATCTACAAGAAGCCAGAGGTAAATTGGGGC
TCCTCGGTACAGGCTGGCTCCTACAACCTGGCCCTCAGCTACTCGGTGGG
CCTCAATGAGGTGGAAGACCACATCAAGAACTACCGCCCCCAGTGCCTGG
TGCTCACGGGGCCCCCCAACTTCCGCCCGGCCCTGGTGGACTTTGTGGGC
ACCTTCACCCGGAACCTCAGCCTGATGATCTGTGGCCACGTGCTCATCGG
ACCCCACAAGCAGAGGATGCCTGAGCTCCAGCTCATCGCCAACGGGCACA
CCAAGTGGCTGAACAAGAGGAAGATCAAGGCCTTCTACTCGGATGTCATT
GCTGAGGACCTCCGCAGAGGCGTCCAGATCCTCATGCAGGCCGCAGGTCT
CGGGAGAATGAAGCCCAACATTCTGGTGGTTGGGTTCAAGAAGAACTGGC
AGTCGGCTCACCCGGCCACAGTGGAAGACTACATTGGCATCCTCCATGAT
GCCTTTGATTTCAACTATGGCGTGTGTGTCATGAGGATGCGGGAGGGACT
CAACGTGTCCAAGATGATGCAGGCGCACATTAACCCCGTGTTTGACCCAG
CGGAGGACGGGAAGGAAGCCAGCGCCAGAGTGGACCCCAAGGCCCTGGTG
AAGGAGGAGCAGGCCACCACCATCTTCCAGTCGGAGCAGGGCAAGAAGAC
CATAGACATCTACTGGCTCTTTGACGATGGAGGCCTCACCCTCCTCATTC
CCTATCTCCTTGGCCGCAAGAGGAGGTGGAGCAAATGCAAGATCCGTGTG
TTCGTAGGTGGCCAGATTAACAGGATGGACCAGGAGAGAAAGGCGATCAT
TTCTCTGCTGAGCAAGTTCCGACTGGGATTCCATGAAGTCCACATCCTCC
CTGACATCAACCAGAACCCTCGGGCTGAGCACACCAAGAGGTTTGAGGAC
ATGATTGCACCCTTCCGTCTGAATGATGGCTTCAAGGATGAGGCCACTGT
CAACGAGATGCGGCGGGACTGCCCCTGGAAGATCTCAGATGAGGAGATTA
CGAAGAACAGAGTCAAGTCCCTTCGGCAGGTGAGGCTGAATGAGATTGTG
CTGGATTACTCCCGAGACGCTGCTCTCATCGTCATCACTTTGCCCATAGG
GAGGAAGGGGAAGTGCCCCAGCTCGCTGTACATGGCCTGGCTGGAGACCC
TGTCCCAGGACCTCAGACCTCCAGTCATCCTGATCCGAGGAAACCAGGAA
AACGTGCTCACCTTTTACTGCCAGTAAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSMAELPTTETPGDA
TLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDV
VPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEM
TDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWIT
AQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGP
ELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRII
GVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKA
SKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGD
LKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGW
GACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFG
ATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAY
AIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQ
YYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWG
SSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVG
TFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVI
AEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHD
AFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARVDPKALV
KEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRV
FVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFED
MIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIV
LDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQE
NVLTFYCQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4464
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST; READ: This protein was amplified by RT-PCR from placenta RNA. All the transcripts variants analyzed had V459A and V460G. These changes differ from accession P55017.