cDNA Clone KIAA0731

• Plasmid: pEBG6P-1 KIAA0731
• Parent Plasmid: pEBG6P
• Expressed: GST-KIAA0731
• Genbank: AB018274 (GI:3882183)
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCGCCGCGGATCGCGTGGTCTGCTTGGCT
CGCCGCGCCTCTCCCCCGTCCGTCCATATTGCTGCAGCCCCCGAGCCGGG
AAGCCCGGGCCGCCCCGGGGGCGGGGGGGAGGGAGGGACGGGACTAGAAG
CCTGCCGGGCTCGGGGTGGGGGCGTCTTGCGAGGAACGGGCGGGGGGGGA
CGCACGCCTAGGAGGCCTGGACTGCAGAGTGGGGGGCCTTCCTCCCCCCC
CGCCCCGCTAGTGGGCCTCGGATTTACGGCGGCTGCATCTAACTGGGAGG
GGGCCGCACCTCGCGTGAACCCCGACCCTTCTCTGCAGGGACTGGGGCCC
AGCGCCCCGGAGGAAGGCGTCGCGGGCGCTCTGCTAGCCAAGGTGAACCC
GTGGACTAAGAACGCATTGCCGCCGGTCCTGACCACCGTGAACGGACAGT
CCCCCCCAGAACACTCTGCTCCAGCCAAGGTGGTGAGGGCAGCTGTTCCT
AAACAGCGCAAAGGCAGCAAGGTTGGTGACTTTGGAGATGCAATCAATTG
GCCCACACCTGGAGAGATAGCCCACAAGAGTGTTCAGCCACAGTCCCACA
AGCCTCAGCCTACCCGTAAACTGCCACCCAAGAAGGACATGAAGGAACAG
GAGAAAGGAGAAGGGAGTGATAGTAAGGAGAGTCCAAAAACCAAATCAGA
TGAATCAGGGGAGGAAAAGAATGGAGATGAGGATTGCCAGCGAGGCGGGC
AGAAGAAGAAAGGAAACAAACACAAGTGGGTTCCATTACAAATAGACATG
AAGCCTGAAGTGCCCAGAGAGAAACTGGCTTCACGCCCCACTCGCCCACC
GGAGCCTAGACACATACCTGCCAATCGCGGAGAGATCAAAGGGTCTGAGT
CTGCCACCTACGTGCCCGTGGCCCCCCCCACCCCAGCCTGGCAACCAGAG
ATCAAACCGGAGCCTGCCTGGCACGACCAGGATGAGACATCGAGTGTGAA
GAGTGATGGGGCTGGTGGGGCGCGGGCTTCCTTCCGTGGCCGTGGACGGG
GGCGTGGTCGCGGCCGGGGACGCGGCCGGGGTGGCACTCGAACCCATTTT
GACTACCAGTTTGGCTACCGAAAGTTTGATGGTGTGGAGGGGCCTCGTAC
GCCCAAGTACATGAACAACATCACCTACTACTTTGACAATGTCAGCAGCA
CCGAGCTTTACAGTGTGGATCAGGAACTGCTCAAAGACTACATCAAGCGC
CAGATTGAATACTACTTCAGCGTGGACAATTTAGAGCGAGACTTCTTCCT
GCGAAGGAAAATGGATGCTGATGGTTTCCTACCCATCACCCTTATTGCTT
CCTTCCACCGAGTGCAGGCCCTTACCACTGACATTTCACTCATCTTTGCG
GCCCTAAAGGACAGCAAGGTGGTGGAGATCGTTGATGAGAAAGTTCGTAG
GAGGGAGGAACCAGAAAAGTGGCCTCTTCCCCCAATAGTGGATTATTCAC
AGACTGATTTCTCCCAGCTTCTCAACTGCCCTGAATTTGTTCCCCGTCAG
CACTACCAAAAGGAGACAGAGTCGGCACCTGGCTCTCCTCGTGCAGTCAC
CCCAGTGCCAACCAAAACAGAGGAGGTCAGCAACCTAAAGACACTACCCA
AGGGCCTGTCTGCCAGCCTGCCTGACCTGGATTCTGAGAACTGGATTGAA
GTGAAGAAGAGGCCTCGGCCATCCCCAGCACGGCCCAAGAAGTCAGAGGA
GTCCAGATTTTCCCACCTGACCTCTCTGCCTCAGCAGCTGCCTTCCCAGC
AGCTGATGTCCAAGGATCAGGATGAGCAAGAGGAACTGGATTTTCTGTTT
GACGAGGAGATGGAGCAGATGGATGGGCGGAAGAACACCTTCACTGCCTG
GTCTGATGAGGAATCTGACTATGAGATTGATGACAGGGATGTCAACAAGA
TCCTCATTGTCACCCAGACACCACATTACATGCGCCGGCACCCAGGGGGG
GACCGCACAGGCAACCACACCTCGCGTGCCAAGATGAGCGCCGAACTGGC
CAAGGTCATTAATGATGGCCTCTTCTACTATGAGCAGGACCTGTGGGCTG
AAAAGTTTGAACCTGAGTATTCCCAGATCAAGCAAGAAGTCGAGAACTTC
AAAAAGGTCAATATGATCAGCCGGGAGCAGTTTGACACACTGACCCCTGA
GCCCCCTGTGGATCCCAACCAGGAAGTTCCTCCTGGGCCACCTCGGTTCC
AGCAAGTTCCTACGGATGCCCTGGCCAACAAGTTGTTTGGTGCTCCTGAG
CCCTCCACCATCGCCCGCTCTCTACCAACCACTGTCCCAGAGTCACCAAA
CTACCGCAACACCAGGACCCCTCGCACTCCCCGGACACCACAGCTCAAAG
ACTCAAGCCAGACATCACGGTTTTACCCAGTGGTGAAAGAAGGACGGACA
CTGGATGCCAAGATGCCTCGAAAAAGAAAGACAAGACACAGTTCAAACCC
ACCCTTGGAGAGCCATGTGGGCTGGGTGATGGATTCCCGTGAGCACAGGC
CCCGTACTGCTTCCATCAGCTCCAGCCCCTCAGAAGGGACGCCTACAGTT
GGCAGCTATGGCTGTACCCCTCAGTCATTGCCCAAGTTCCAGCATCCTTC
CCATGAACTGCTCAAGGAAAATGGCTTCACACAACACGTCTACCATAAGT
ATCGTAGGCGCTGCCTTAATGAGCGGAAACGCTTGGGCATTGGCCAGTCT
CAGGAGATGAACACACTCTTCCGCTTCTGGTCCTTCTTCCTCCGAGATCA
CTTCAACAAAAAGATGTATGAGGAGTTCAAGCAGCTGGCTCTGGAGGACG
CCAAAGAAGGCTACAGATATGGTTTGGAGTGCCTTTTTCGATACTACAGT
TATGGCCTGGAAAAGAAGTTCCGGCTGGACATATTCAAGGATTTTCAGGA
GGAAACGGTGAAGGACTATGAAGCTGGCCAACTGTATGGGCTGGAGAAGT
TCTGGGCCTTCTTGAAATATTCCAAAGCCAAAAATTTGGACATTGACCCC
AAACTGCAAGAATACCTCGGCAAATTCCGACGTCTTGAAGACTTCCGAGT
AGATCCCCCCATGGGTGAGGAGGGCAACCACAAGCGACACTCAGTGGTAG
CAGGAGGTGGCGGCGGTGAGGGCAGGAAGCGGTGCCCCTCCCAGTCTTCC
AGCAGGCCTGCTGCCATGATCAGCCAACCCCCTACACCACCCACCGGCCA
GCCTGTCCGGGAAGATGCCAAATGGACAAGCCAGCACTCGAACACACAGA
CTTTGGGAAAGTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTAADRVVCLA
RRASPPSVHIAAAPEPGSPGRPGGGGEGGTGLEACRARGGGVLRGTGGGG
RTPRRPGLQSGGPSSPPAPLVGLGFTAAASNWEGAAPRVNPDPSLQGLGP
SAPEEGVAGALLAKVNPWTKNALPPVLTTVNGQSPPEHSAPAKVVRAAVP
KQRKGSKVGDFGDAINWPTPGEIAHKSVQPQSHKPQPTRKLPPKKDMKEQ
EKGEGSDSKESPKTKSDESGEEKNGDEDCQRGGQKKKGNKHKWVPLQIDM
KPEVPREKLASRPTRPPEPRHIPANRGEIKGSESATYVPVAPPTPAWQPE
IKPEPAWHDQDETSSVKSDGAGGARASFRGRGRGRGRGRGRGRGGTRTHF
DYQFGYRKFDGVEGPRTPKYMNNITYYFDNVSSTELYSVDQELLKDYIKR
QIEYYFSVDNLERDFFLRRKMDADGFLPITLIASFHRVQALTTDISLIFA
ALKDSKVVEIVDEKVRRREEPEKWPLPPIVDYSQTDFSQLLNCPEFVPRQ
HYQKETESAPGSPRAVTPVPTKTEEVSNLKTLPKGLSASLPDLDSENWIE
VKKRPRPSPARPKKSEESRFSHLTSLPQQLPSQQLMSKDQDEQEELDFLF
DEEMEQMDGRKNTFTAWSDEESDYEIDDRDVNKILIVTQTPHYMRRHPGG
DRTGNHTSRAKMSAELAKVINDGLFYYEQDLWAEKFEPEYSQIKQEVENF
KKVNMISREQFDTLTPEPPVDPNQEVPPGPPRFQQVPTDALANKLFGAPE
PSTIARSLPTTVPESPNYRNTRTPRTPRTPQLKDSSQTSRFYPVVKEGRT
LDAKMPRKRKTRHSSNPPLESHVGWVMDSREHRPRTASISSSPSEGTPTV
GSYGCTPQSLPKFQHPSHELLKENGFTQHVYHKYRRRCLNERKRLGIGQS
QEMNTLFRFWSFFLRDHFNKKMYEEFKQLALEDAKEGYRYGLECLFRYYS
YGLEKKFRLDIFKDFQEETVKDYEAGQLYGLEKFWAFLKYSKAKNLDIDP
KLQEYLGKFRRLEDFRVDPPMGEEGNHKRHSVVAGGGGGEGRKRCPSQSS
SRPAAMISQPPTPPTGQPVREDAKWTSQHSNTQTLGK*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4426
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST tag.