cDNA Clone GYS2 (mouse)

• Plasmid: pEBG6P-1 GYS2
• Parent Plasmid: pEBG6P
• Expressed: GST-GYS2
• Genbank: NM_145572
• Tag: GST
• Species: Mouse

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCATGCTCAGAGGCCGCTCCTTGTCGGTG
ACATCCCTTGGTGGGCTCCCTGTGTGGGAAGCTGAAAGACTCCCTGTGGA
AGACTTACTCCTTTTTGAAGTTTCTTGGGAGGTGACCAACAAAGTTGGGG
GCATCTGTACTGTGATCCAGACCAAGGCCAAAACGACAGCCGATGAGTGG
GGAGAGAATTACTTCCTGATAGGTCCGTACTTTGAGCATAATATGAAGAC
TCAAGTAGAACAATGTGAGCCCACCAACGATGCTGTCAGAAAAGCTGTGG
ATGCGATGAATAAACATGGCTGCCAGGTGCATTTTGGAAGATGGCTCATA
GAAGGGAGTCCCTACGTGGTGCTCTTTGACATCAGCTCCTCAGCATGGAA
CCTGGACAGATGGAAGGGTGACTTCTGGGAAGCTTGTGGCGTTGGCATCC
CTCATCATGACCGAGAAGCTAACGACATGCTCATATTTGGGTCTTTAACT
GCCTGGTTCTTAAAGGAGGTGACAGACCACGCAGACGGTAAACACGTCAT
TGCCCAATTCCATGAATGGCAGGCTGGGACTGGGCTGATCCTTTCTCGTG
CCAGGAAACTCCCCATTGCCACAGTATTTACAACCCATGCCACACTGCTT
GGGCGTTATCTCTGTGCAGCAAATATTGACTTCTACAACCAGCTTGACAA
GTTCGACATAGACAAAGAGGCCGGGGAGAGGCAGATATACCACCGCTACT
GCATGGAGCGGGCATCCGTGCACTGTGCGCACGTGTTCACCACAGTGTCG
GAAATCACAGCCATCGAGGCAGAGCACATGCTGAAGAGGAAGCCTGATGT
AGTGACTCCAAATGGTTTGAATGTTAAGAAGTTTTCTGCAGTGCATGAAT
TTCAAAATCTCCACGCCATGTACAAGGCCAGGATACAGGATTTCGTTCGA
GGTCATTTCTATGGTCATCTGGACTTTGATCTTGAGAAGACTTTATTCCT
CTTCATTGCTGGGAGATATGAATTCTCAAACAAAGGAGCAGACATCTTCC
TGGAGTCCTTATCCAGGCTTAATTTCCTCCTGAGGATGCATAAGAGTAAC
GTCACCGTGGTAGTGTTTTTCATCATGCCTGCCAAGACAAACAATTTCAA
CGTGGAAACCCTGAAGGGCCAGGCAGTGCGGAAACAGCTGTGGGACACTG
TGCATTGTTTGAAGGAGAAGTTTGGGAAGAAACTCTATGACGGGTTATTA
AGAGGAGAAATTCCTGACATGAATAGTATTTTGGATCGAGATGACTTAAC
AATTATGAAAAGGGCCATCTTTTCAACTCAGAGACAGTCTTTGCCTCCTG
TGACCACTCACAATATGATCGATGATTCCACGGATCCCATCCTCAGCACC
ATTAGACGAATCGGACTTTTCAACAACCGTGCAGACAGAGTCAAGGTGAT
TTTACACCCAGAATTCCTGTCCTCCACCAGCCCCCTATTGCCCATGGATT
ATGAAGAGTTTGTTCGAGGTTGTCACCTTGGGGTGTTTCCATCGTACTAT
GAACCCTGGGGTTACACACCAGCTGAATGCACAGTGATGGGCATCCCCAG
TGTGACTACAAACCTCTCCGGTTTCGGGTGTTTCGTGCAGGAGCATGTGG
CTGACCCTACTGCATACGGTATTTACATCGTGGACAGACGCTTCCGCTCT
CCAGACGATTCTTGCAACCAACTGACTCAGTTCCTCTACGGGTTTTGTAA
ACAGTCACGCCGGCAAAGGATCATTCAGAGGAACCGCACGGAGAGGCTCT
CAGATCTCCTGGACTGGAGATACCTGGGCAGATATTACCAGCATGCCAGA
CACCTGACACTGAGCAGGGCTTTTCCAGACAAATTCCACCTAGAGCCCAC
ATCACCGCCAATGACGGATGGCTTTAAGTATCCCAGGCCCTCCTCAGTAC
CACCTTCTCCGTCAGGATCCCAGGCCTCCAGTCCTCAGAGCAGTGATGCA
GAAGATGAAGAAGATGAGGATGAGAGGTATGATGAGGAAGAGGAGGCTGA
GAGGGATCGGCTAAATATCAAGTCACCGTTTTCTCTGAACCACTTTCCAA
AGGGGAAGAAAAAGCTTCATGGAGAATATAAGAACTGAGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTMLRGRSLSV
TSLGGLPVWEAERLPVEDLLLFEVSWEVTNKVGGICTVIQTKAKTTADEW
GENYFLIGPYFEHNMKTQVEQCEPTNDAVRKAVDAMNKHGCQVHFGRWLI
EGSPYVVLFDISSSAWNLDRWKGDFWEACGVGIPHHDREANDMLIFGSLT
AWFLKEVTDHADGKHVIAQFHEWQAGTGLILSRARKLPIATVFTTHATLL
GRYLCAANIDFYNQLDKFDIDKEAGERQIYHRYCMERASVHCAHVFTTVS
EITAIEAEHMLKRKPDVVTPNGLNVKKFSAVHEFQNLHAMYKARIQDFVR
GHFYGHLDFDLEKTLFLFIAGRYEFSNKGADIFLESLSRLNFLLRMHKSN
VTVVVFFIMPAKTNNFNVETLKGQAVRKQLWDTVHCLKEKFGKKLYDGLL
RGEIPDMNSILDRDDLTIMKRAIFSTQRQSLPPVTTHNMIDDSTDPILST
IRRIGLFNNRADRVKVILHPEFLSSTSPLLPMDYEEFVRGCHLGVFPSYY
EPWGYTPAECTVMGIPSVTTNLSGFGCFVQEHVADPTAYGIYIVDRRFRS
PDDSCNQLTQFLYGFCKQSRRQRIIQRNRTERLSDLLDWRYLGRYYQHAR
HLTLSRAFPDKFHLEPTSPPMTDGFKYPRPSSVPPSPSGSQASSPQSSDA
EDEEDEDERYDEEEEAERDRLNIKSPFSLNHFPKGKKKLHGEYKN*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4356
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N terminal GST.