cDNA Clone sec23A

• Plasmid: pGEX6P-1-sec23A delta 580-603
• Parent Plasmid: pGEX6P1
• Expressed: GST-sec23A delta 580-603
• Genbank: NP_006355
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCACAACCT
ATTTGGAATTCATTCAACAAAATGAAGAACGAGATGGAGTCCGATTTAGT
TGGAATGTTTGGCCATCAAGTCGACTGGAAGCTACAAGAATGGTTGTTCC
TGTGGCAGCCCTGTTTACACCACTGAAAGAGAGACCTGACTTACCACCTA
TTCAATATGAACCTGTTCTGTGTAGTAGGACCACTTGCCGTGCAGTTTTG
AATCCTTTATGTCAAGTGGATTATCGAGCAAAACTTTGGGCTTGCAACTT
TTGTTACCAAAGGAATCAGTTTCCACCTAGTTATGCTGGTATATCTGAAC
TGAATCAGCCTGCTGAACTTTTACCTCAGTTTTCTAGCATTGAATATGTA
GTTCTGCGTGGTCCTCAGATGCCTTTGATATTCCTCTATGTGGTTGATAC
TTGCATGGAAGATGAAGATTTACAAGCCCTGAAAGAATCCATGCAGATGT
CATTAAGTCTTTTACCACCTACAGCTTTGGTTGGACTTATTACTTTTGGG
AGAATGGTTCAGGTTCATGAACTTGGATGTGAAGGCATTTCAAAAAGCTA
TGTCTTCAGAGGAACAAAAGATTTGTCTGCCAAACAACTGCAGGAAATGC
TGGGGCTCTCTAAAGTACCACTTACTCAAGCAACACGTGGTCCTCAGGTA
CAGCAGCCACCTCCTTCCAACAGATTCTTACAACCAGTACAGAAAATAGA
CATGAATCTCACAGATCTTCTGGGAGAACTCCAGCGAGACCCTTGGCCTG
TACCACAGGGAAAGAGACCTTTGCGTTCCTCTGGGGTGGCACTTTCCATA
GCTGTAGGACTGCTGGAGTGTACTTTTCCCAACACTGGTGCTCGTATCAT
GATGTTCATTGGTGGTCCTGCTACTCAGGGGCCTGGAATGGTGGTTGGAG
ATGAGTTGAAGACACCTATAAGATCGTGGCATGACATTGACAAAGACAAT
GCCAAATATGTTAAAAAGGGAACTAAGCATTTTGAAGCATTGGCTAATCG
AGCTGCTACAACTGGCCATGTTATTGATATCTATGCGTGTGCATTAGATC
AGACAGGTCTCCTGGAGATGAAATGCTGTCCCAACCTTACTGGAGGATAC
ATGGTAATGGGTGATTCTTTCAATACTTCCTTATTCAAACAAACTTTTCA
AAGAGTCTTTACCAAAGACATGCATGGACAGTTTAAAATGGGCTTTGGTG
GTACGCTAGAAATAAAGACCTCAAGGGAAATAAAGATTTCAGGAGCTATT
GGACCCTGTGTGTCACTCAATTCTAAAGGACCCTGTGTGTCTGAAAATGA
GATAGGAACAGGTGGCACATGTCAGTGGAAGATATGTGGACTTAGTCCCA
CTACAACCTTAGCCATATATTTTGAGGTTGTCAATCAGCATAATGCTCCA
ATTCCTCAAGGAGGGCGTGGTGCAATCCAGTTTGTGACTCAGTATCAGCA
TTCAAGTGGGCAGAGACGCATCCGAGTGACCACCATTGCTCGGAACTGGG
CAGATGCTCAAACTCAAATCCAAAACATTGCTGCATCTTTTGACCAGGAG
GCAGCTGCCATTCTTATGGCCCGGCTAGCAATATATAGAGCAGAAACAGA
AGAAGGTCCAGATGTGCTTAGGTGGCTGGACAGACAGCTCATTCGACTGT
GTCAGAAATTTGGAGAATATCATAAAGATGACCCAAGTTCCTTCAGATTT
TCAGAAACTTTCTCCCTTTATCCACAGTTTATGTTTCATTTAAGAAGATC
TTCTTTCCTGCAAGTTTTTAACAATAGTCCTGATGAGAGTTCATATTATC
GTCACCATTTTATGCGTCAAGATCTGACCCAGTCTCTAATTATGATTCAG
CCTATCCTGTATGCGTATTCCTTTAGTGGACCACCAGAGCCGGTTCTTCT
TGATAGCAGTAGCATTCTTGCAGATCGTATTCTTCTCATGGACACATTCT
TCCAGATTTTGATTTATCATGGTGAGACCATAGCACAGTGGCGGAAGTCA
GGATACCAGGATATGCCTGAGTATGAAAATTTCCGCCACCTTCTGCAAGC
CCCAGTGGATGATGCACAGGAAATTCTTCACTCCAGATTTCCAATGCCAA
GATACATTGACACTGAACATGGAGGCAGCCAGGCCCGTTTCCTCCTTTCA
AAAGTCAACCCTTCACAGACTCATAATAATATGTATGCCTGGGGGCAGGA
GTCTGGAGCACCTATTCTTACAGATGATGTTAGTTTACAAGTGTTTATGG
ATCACTTGAAGAAACTTGCTGTGTCCAGTGCTGCTTGATCTAGAGCAAGG
GCGAATTCCAGCACACTGGCGGCCGTTACTAGTGGATCCCCGGAATTCCC
GGGTCGACTCGAGCGGCCGCATC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSTTYLEFIQQNEERDGVRFS
WNVWPSSRLEATRMVVPVAALFTPLKERPDLPPIQYEPVLCSRTTCRAVL
NPLCQVDYRAKLWACNFCYQRNQFPPSYAGISELNQPAELLPQFSSIEYV
VLRGPQMPLIFLYVVDTCMEDEDLQALKESMQMSLSLLPPTALVGLITFG
RMVQVHELGCEGISKSYVFRGTKDLSAKQLQEMLGLSKVPLTQATRGPQV
QQPPPSNRFLQPVQKIDMNLTDLLGELQRDPWPVPQGKRPLRSSGVALSI
AVGLLECTFPNTGARIMMFIGGPATQGPGMVVGDELKTPIRSWHDIDKDN
AKYVKKGTKHFEALANRAATTGHVIDIYACALDQTGLLEMKCCPNLTGGY
MVMGDSFNTSLFKQTFQRVFTKDMHGQFKMGFGGTLEIKTSREIKISGAI
GPCVSLNSKGPCVSENEIGTGGTCQWKICGLSPTTTLAIYFEVVNQHNAP
IPQGGRGAIQFVTQYQHSSGQRRIRVTTIARNWADAQTQIQNIAASFDQE
AAAILMARLAIYRAETEEGPDVLRWLDRQLIRLCQKFGEYHKDDPSSFRF
SETFSLYPQFMFHLRRSSFLQVFNNSPDESSYYRHHFMRQDLTQSLIMIQ
PILYAYSFSGPPEPVLLDSSSILADRILLMDTFFQILIYHGETIAQWRKS
GYQDMPEYENFRHLLQAPVDDAQEILHSRFPMPRYIDTEHGGSQARFLLS
KVNPSQTHNNMYAWGQESGAPILTDDVSLQVFMDHLKKLAVSSAA*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4313
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Note that this clone is referred to this way to differentiate it from sec23A FL. NP_006355.