cDNA Clone FLJ34154

• Plasmid: pEBG6P-1 FLJ34154
• Parent Plasmid: pEBG6P
• Expressed: GST-FLJ34154
• Genbank: BC065706
• Tag: GST
• Species: Human

Sequence of Insert

ATGGCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCTGCATACACCAACTCAATCTTCTTGCCACCAACCCCAAT
CTTCCAATCACAAGTGTCTTGGGCAAGCAGCATCCAATTGAAGCACATCA
TCTTAGCAGTATTTGTGACATTATGGAGAAGGCCATGGTTAATGGAGATA
CCTGTATTATACGCTGCATTCTCGTTGTCTTTCAGGTGGTATTTAAATTT
TTCTTCAGCCCACAAACTGAAAGGAATCGAGACATCATTCGACGGTCGGG
ATTGCTTCTTTGGCAGTTGTTGATGGCTCCAAAAGATCAAATTTGCCCTG
AAATTCAGAAGGAAGTCTGCCTTGCCATCAGCTCTGGTTTAAATATCTTG
TACCCAGGTGAAACTGAAATCAATAACTTACTTAAACTGGTCTTAACAGA
AGGAGAGAGAAACAGTGGACTCTCCCAGCTACGGGATGTGATCCTAACCA
ACCTGGCTGAACAGCTCCAAAACAACCGATTTGGCAGTGATGAGGATGAT
CATTACAGACTAAATGATGAACTTTTACACTACATTCTGAAGATTGTTGT
ACGAGAATCCTGTATCTTAATCACCAAGTGCCAAACTGTCTCTAAAGATG
ATTTTCAAAAGCTCCTTTCAACTGTGCCTGCTGCATCCTCCTGCCTGCGC
TATCTGATGGCAGTTCAGAATCACCTTCTCAGTAACACTATTTTGATTAA
ACCTGATGAGAATGATGACAGTGACAGCTCCTTGCAGGGAGAGACATTGA
AGGAGCTAAAAGTCAGTATTTTGGCTCTTGCCACCCAAATCCTGACTGGA
TGTGATGAAGTGTTGGAAATGCTACAGCAGGTCACAACTGCCCTCATAAA
TAGTGACATAGCAGACCGTGAGCAGAGGTTAAAAGGCTTGGAACAAGTTA
CTAAGGCTACTATGCTTGGTCACCTTCTTCCAGTGTTACTGACCTCCTTG
ATGCATCCAAATTTACAGACTCTGATCATGGCGGATGCCCTGATGCCTCA
GCTAGTGCAGCTGGTACTCTATACCAGCCAGACGGCGTTGCTGCTTAAAA
CCCAGTGTCCGGTTTTTGCTGAGGTGGGCTGTTCCCCGTGTGGTGCACCA
GACCAGAAGTGCAGGCTGTTCCCTGATGAGAGAATGTTAGAAGAGAAGGA
AGAGCCAGGATTTCTCACTGGTTTAAAGATTCCTGCCCCATGGGCTGCTG
GAAAGACTGTGGAAACAGTCCACCCCGTCAGAGACAACTATAAATTTAAA
GAAACGGTCCATATCCCAGGAGCTCGCTGCCTGTACCTTAGATTTGATAG
CAGATGCTCTTCGCAATATGACTATGACAAATTGGTGATATATGCGGGGC
CTAACACAAACAGTAGGAAGGTTGCTGAATATGGAGGCAACACACTGGGA
TATGGCAGCCGTAGTGTCTTAGGAACTGGTTGGCCGAAAGACTTGGTGAA
GGTGGAAGGAGATACAGTCACCTTCTCCTTTGAAATGAGAAGTGGCCGTG
AACACAACACTCCTGATAAAGCCATGTGGGGCTTTGCTTGCACAGTTCGC
GCTCAGGAGTCTTCGGAGGATGTCTCAGGAGGCTTGCCCTTTCTGGTAGA
CCTGGCTTTAGGTCTGTCTGTGTTAGCTTGTTCCATGTTAAGAATCCTGT
ACAATGGACCAGAAATTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
CATGTCGGCCGCCTCGGCCCTCGAGAAGCTTTCTAGGCGGCCGCG

Amino Acid Sequence

MAPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSCIHQLNLLATNPN
LPITSVLGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKF
FFSPQTERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNIL
YPGETEINNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDD
HYRLNDELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLR
YLMAVQNHLLSNTILIKPDENDDSDSSLQGETLKELKVSILALATQILTG
CDEVLEMLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSL
MHPNLQTLIMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCSPCGAP
DQKCRLFPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFK
ETVHIPGARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLG
YGSRSVLGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVR
AQESSEDVSGGLPFLVDLALGLSVLACSMLRILYNGPEITEKKKKKKKKK
HVGRLGPREAF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4285
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression with N term GST.