cDNA Clone FLJ34154

• Plasmid: pGEX6P-1-FLJ34154
• Parent Plasmid: pGEX6P1
• Expressed: GST-FLJ34154
• Genbank: BC065706
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCTGCATAC
ACCAACTCAATCTTCTTGCCACCAACCCCAATCTTCCAATCACAAGTGTC
TTGGGCAAGCAGCATCCAATTGAAGCACATCATCTTAGCAGTATTTGTGA
CATTATGGAGAAGGCCATGGTTAATGGAGATACCTGTATTATACGCTGCA
TTCTCGTTGTCTTTCAGGTGGTATTTAAATTTTTCTTCAGCCCACAAACT
GAAAGGAATCGAGACATCATTCGACGGTCGGGATTGCTTCTTTGGCAGTT
GTTGATGGCTCCAAAAGATCAAATTTGCCCTGAAATTCAGAAGGAAGTCT
GCCTTGCCATCAGCTCTGGTTTAAATATCTTGTACCCAGGTGAAACTGAA
ATCAATAACTTACTTAAACTGGTCTTAACAGAAGGAGAGAGAAACAGTGG
ACTCTCCCAGCTACGGGATGTGATCCTAACCAACCTGGCTGAACAGCTCC
AAAACAACCGATTTGGCAGTGATGAGGATGATCATTACAGACTAAATGAT
GAACTTTTACACTACATTCTGAAGATTGTTGTACGAGAATCCTGTATCTT
AATCACCAAGTGCCAAACTGTCTCTAAAGATGATTTTCAAAAGCTCCTTT
CAACTGTGCCTGCTGCATCCTCCTGCCTGCGCTATCTGATGGCAGTTCAG
AATCACCTTCTCAGTAACACTATTTTGATTAAACCTGATGAGAATGATGA
CAGTGACAGCTCCTTGCAGGGAGAGACATTGAAGGAGCTAAAAGTCAGTA
TTTTGGCTCTTGCCACCCAAATCCTGACTGGATGTGATGAAGTGTTGGAA
ATGCTACAGCAGGTCACAACTGCCCTCATAAATAGTGACATAGCAGACCG
TGAGCAGAGGTTAAAAGGCTTGGAACAAGTTACTAAGGCTACTATGCTTG
GTCACCTTCTTCCAGTGTTACTGACCTCCTTGATGCATCCAAATTTACAG
ACTCTGATCATGGCGGATGCCCTGATGCCTCAGCTAGTGCAGCTGGTACT
CTATACCAGCCAGACGGCGTTGCTGCTTAAAACCCAGTGTCCGGTTTTTG
CTGAGGTGGGCTGTTCCCCGTGTGGTGCACCAGACCAGAAGTGCAGGCTG
TTCCCTGATGAGAGAATGTTAGAAGAGAAGGAAGAGCCAGGATTTCTCAC
TGGTTTAAAGATTCCTGCCCCATGGGCTGCTGGAAAGACTGTGGAAACAG
TCCACCCCGTCAGAGACAACTATAAATTTAAAGAAACGGTCCATATCCCA
GGAGCTCGCTGCCTGTACCTTAGATTTGATAGCAGATGCTCTTCGCAATA
TGACTATGACAAATTGGTGATATATGCGGGGCCTAACACAAACAGTAGGA
AGGTTGCTGAATATGGAGGCAACACACTGGGATATGGCAGCCGTAGTGTC
TTAGGAACTGGTTGGCCGAAAGACTTGGTGAAGGTGGAAGGAGATACAGT
CACCTTCTCCTTTGAAATGAGAAGTGGCCGTGAACACAACACTCCTGATA
AAGCCATGTGGGGCTTTGCTTGCACAGTTCGCGCTCAGGAGTCTTCGGAG
GATGTCTCAGGAGGCTTGCCCTTTCTGGTAGACCTGGCTTTAGGTCTGTC
TGTGTTAGCTTGTTCCATGTTAAGAATCCTGTACAATGGACCAGAAATTA
CCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATGTCGGCCGCCTCGGC
CCTCGAGAAGCTTTCTAGGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSCIHQLNLLATNPNLPITSV
LGKQHPIEAHHLSSICDIMEKAMVNGDTCIIRCILVVFQVVFKFFFSPQT
ERNRDIIRRSGLLLWQLLMAPKDQICPEIQKEVCLAISSGLNILYPGETE
INNLLKLVLTEGERNSGLSQLRDVILTNLAEQLQNNRFGSDEDDHYRLND
ELLHYILKIVVRESCILITKCQTVSKDDFQKLLSTVPAASSCLRYLMAVQ
NHLLSNTILIKPDENDDSDSSLQGETLKELKVSILALATQILTGCDEVLE
MLQQVTTALINSDIADREQRLKGLEQVTKATMLGHLLPVLLTSLMHPNLQ
TLIMADALMPQLVQLVLYTSQTALLLKTQCPVFAEVGCSPCGAPDQKCRL
FPDERMLEEKEEPGFLTGLKIPAPWAAGKTVETVHPVRDNYKFKETVHIP
GARCLYLRFDSRCSSQYDYDKLVIYAGPNTNSRKVAEYGGNTLGYGSRSV
LGTGWPKDLVKVEGDTVTFSFEMRSGREHNTPDKAMWGFACTVRAQESSE
DVSGGLPFLVDLALGLSVLACSMLRILYNGPEITEKKKKKKKKKHVGRLG
PREAF*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4284
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N term GST.