cDNA Clone hn RNP K isoform b

• Plasmid: pCMV5-HA hn RNP K S116A isoform b
• Parent Plasmid: pCMV5
• Expressed: HA-hn RNP K S116A isoform b
• Genbank: AAH14980
• Tag: HA
• Species: Human

Sequence of Insert

GAATTCGCCACCATGTACCCATACGATGTGCCAGATTACGCCGAAACTGA
ACAGCCAGAAGAAACCTTCCCTAACACTGAAACCAATGGTGAATTTGGTA
AACGCCCTGCAGAAGATATGGAAGAGGAACAAGCATTTAAAAGATCTAGA
AACACTGATGAGATGGTTGAATTACGCATTCTGCTTCAGAGCAAGAATGC
TGGGGCAGTGATTGGAAAAGGAGGCAAGAATATTAAGGCTCTCCGTACAG
ACTACAATGCCAGTGTTTCAGTCCCAGACAGCAGTGGCCCCGAGCGCATA
TTGAGTATCAGTGCTGATATTGAAACAATTGGAGAAATTCTGAAGAAAAT
CATCCCTACCTTGGAAGAGGGCCTGCAGTTGCCAGCACCCACTGCAACCA
GCCAGCTCCCGCTCGAATCTGATGCTGTGGAATGCTTAAATTACCAACAC
TATAAAGGAAGTGACTTTGACTGCGAGTTGAGGCTGTTGATTCATCAGAG
TCTAGCAGGAGGAATTATTGGGGTCAAAGGTGCTAAAATCAAAGAACTTC
GAGAGAACACTCAAACCACCATCAAGCTTTTCCAGGAATGCTGTCCTCAT
TCCACTGACAGAGTTGTTCTTATTGGAGGAAAACCCGATAGGGTTGTAGA
GTGCATAAAGATCATCCTTGATCTTATATCTGAGTCTCCCATCAAAGGAC
GTGCACAGCCTTATGATCCCAATTTTTACGATGAAACCTATGATTATGGT
GGTTTTACAATGATGTTTGATGACCGTCGCGGACGCCCAGTGGGATTTCC
CATGCGGGGAAGAGGTGGTTTTGACAGAATGCCTCCTGGTCGGGGTGGGC
GTCCCATGCCTCCATCTAGAAGAGATTATGATGATATGAGCCCTCGTCGA
GGACCACCTCCCCCTCCTCCCGGACGAGGCGGCCGGGGTGGTAGCAGAGC
TCGGAATCTTCCTCTTCCTCCACCACCACCACCTAGAGGGGGAGACCTCA
TGGCCTATGACAGAAGAGGGAGACCTGGAGACCGTTACGACGGCATGGTT
GGTTTCAGTGCTGATGAAACTTGGGACTCTGCAATAGATACATGGAGCCC
ATCAGAATGGCAGATGGCTTATGAACCACAGGGTGGCTCCGGATATGATT
ATTCCTATGCAGGGGGTCGTGGCTCATATGGTGATCTTGGTGGACCTATT
ATTACTACACAAGTAACTATTCCCAAAGATTTGGCTGGATCTATTATTGG
CAAAGGTGGTCAGCGGATTAAACAAATCCGTCATGAGTCGGGAGCTTCGA
TCAAAATTGATGAGCCTTTAGAAGGATCCGAAGATCGGATCATTACCATT
ACAGGAACACAGGACCAGATACAGAATGCACAGTATTTGCTGCAGAACAG
TGTGAAGCAGTATTCTGGAAAGTTTTTCTAAGTCGAC

Amino Acid Sequence

MYPYDVPDYAETEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDE
MVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSIS
ADIETIGEILKKIIPTLEEGLQLPAPTATSQLPLESDAVECLNYQHYKGS
DFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDR
VVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTM
MFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPP
PPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSA
DETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQ
VTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQ
DQIQNAQYLLQNSVKQYSGKFF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4184
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Mammalian expression plasmid with N terminus HA tag.