cDNA Clone CDC34

• Synonyms: UBCH3, UBC3, UBE2R1
• Plasmid: pGEX6P-1-CDC34
• Parent Plasmid: pGEX6P1
• Expressed: GST-CDC34
• Genbank: NM_004359
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCGCTCGGC
CGCTAGTGCCCAGCTCGCAGAAGGCGCTGCTGCTGGAGCTCAAGGGGCTG
CAGGAAGAGCCGGTCGAGGGATTCCGCGTGACACTGGTGGACGAGGGCGA
TCTATACAACTGGGAGGTGGCCATCTTCGGGCCCCCCAACACCTACTACG
AGGGCGGCTACTTCAAGGCGCGCCTCAAGTTCCCCATCGACTACCCATAC
TCTCCACCAGCCTTTCGGTTCCTGACCAAGATGTGGCACCCTAACATCTA
CGAGACGGGGGACGTGTGTATCTCCATCCTCCACCCGCCGGTGGACGACC
CCCAGAGCGGGGAGCTGCCCTCAGAGAGGTGGAACCCCACGCAGAACGTC
AGGACCATTCTCCTGAGTGTGATCTCCCTCCTGAACGAGCCCAACACCTT
CTCGCCCGCAAACGTGGACGCCTCCGTGATGTACAGGAAGTGGAAAGAGA
GCAAGGGGAAGGATCGGGAGTACACAGACATCATCCGGAAGCAGGTCCTG
GGGACCAAGGTGGACGCGGAGCGTGACGGCGTGAAGGTGCCCACCACGCT
GGCCGAGTACTGCGTGAAGACCAAGGCGCCGGCGCCCGACGAGGGCTCAG
ACCTCTTCTACGACGACTACTACGAGGACGGCGAGGTGGAGGAGGAGGCC
GACAGCTGCTTCGGGGACGATGAGGATGACTCTGGCACGGAGGAGTCCTG
AGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSARPLVPSSQKALLLELKGL
QEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPY
SPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNV
RTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVL
GTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEA
DSCFGDDEDDSGTEES*

• Vector Type: Bacterial
• Antibiotic: Amp
• Full Snapgene Map: pGEX6P-1-CDC3486 KBSnapgene files can be opened using software available here: www.snapgene.com
• DU Number: DU4164
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST.
• Low Temp: No