cDNA Clone hn RNP K transcript variant 3

• Plasmid: pEBG6P-1 hn RNP K (KpnI/NotI) transcript variant 3
• Parent Plasmid: pGEX6P1
• Expressed: GST-hn RNP K
• Genbank: AAH14980
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGTTCCGCGTGGATCTCTGGAAGTTCTGTTCCAGGGGC
CCCTGGGATCCACTAGTGGTACCGAAACTGAACAGCCAGAAGAAACCTTC
CCTAACACTGAAACCAATGGTGAATTTGGTAAACGCCCTGCAGAAGATAT
GGAAGAGGAACAAGCATTTAAAAGATCTAGAAACACTGATGAGATGGTTG
AATTACGCATTCTGCTTCAGAGCAAGAATGCTGGGGCAGTGATTGGAAAA
GGAGGCAAGAATATTAAGGCTCTCCGTACAGACTACAATGCCAGTGTTTC
AGTCCCAGACAGCAGTGGCCCCGAGCGCATATTGAGTATCAGTGCTGATA
TTGAAACAATTGGAGAAATTCTGAAGAAAATCATCCCTACCTTGGAAGAG
GGCCTGCAGTTGCCATCACCCACTGCAACCAGCCAGCTCCCGCTCGAATC
TGATGCTGTGGAATGCTTAAATTACCAACACTATAAAGGAAGTGACTTTG
ACTGCGAGTTGAGGCTGTTGATTCATCAGAGTCTAGCAGGAGGAATTATT
GGGGTCAAAGGTGCTAAAATCAAAGAACTTCGAGAGAACACTCAAACCAC
CATCAAGCTTTTCCAGGAATGCTGTCCTCATTCCACTGACAGAGTTGTTC
TTATTGGAGGAAAACCCGATAGGGTTGTAGAGTGCATAAAGATCATCCTT
GATCTTATATCTGAGTCTCCCATCAAAGGACGTGCACAGCCTTATGATCC
CAATTTTTACGATGAAACCTATGATTATGGTGGTTTTACAATGATGTTTG
ATGACCGTCGCGGACGCCCAGTGGGATTTCCCATGCGGGGAAGAGGTGGT
TTTGACAGAATGCCTCCTGGTCGGGGTGGGCGTCCCATGCCTCCATCTAG
AAGAGATTATGATGATATGAGCCCTCGTCGAGGACCACCTCCCCCTCCTC
CCGGACGAGGCGGCCGGGGTGGTAGCAGAGCTCGGAATCTTCCTCTTCCT
CCACCACCACCACCTAGAGGGGGAGACCTCATGGCCTATGACAGAAGAGG
GAGACCTGGAGACCGTTACGACGGCATGGTTGGTTTCAGTGCTGATGAAA
CTTGGGACTCTGCAATAGATACATGGAGCCCATCAGAATGGCAGATGGCT
TATGAACCACAGGGTGGCTCCGGATATGATTATTCCTATGCAGGGGGTCG
TGGCTCATATGGTGATCTTGGTGGACCTATTATTACTACACAAGTAACTA
TTCCCAAAGATTTGGCTGGATCTATTATTGGCAAAGGTGGTCAGCGGATT
AAACAAATCCGTCATGAGTCGGGAGCTTCGATCAAAATTGATGAGCCTTT
AGAAGGATCCGAAGATCGGATCATTACCATTACAGGAACACAGGACCAGA
TACAGAATGCACAGTATTTGCTGCAGAACAGTGTGAAGCAGTATGCAGAT
GTTGAAGGATTCTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLVPRGSLEVLFQGPLGSTSGTETEQPEETF
PNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGK
GGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEE
GLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGII
GVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIIL
DLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGG
FDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLP
PPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMA
YEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRI
KQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYAD
VEGF*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU4097
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression plasmid with N terminus GST tag