cDNA Clone hn RNP U

• Plasmid: pGEX6P-1-hn RNP U with xtra 228 Ala
• Parent Plasmid: pGEX6P1
• Expressed: GST-hn RNP U with xtra 228 Ala
• Genbank: NM_031844.1
• Tag: GST
• Species: Human

Sequence of Insert

ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCAC
TCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATG
AGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTG
GAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACA
GTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTG
GTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTG
GATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAAC
TCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCG
AAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCAT
CCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCC
AATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTG
AAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCA
TGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCC
AAAATCGGATCTGGAAGTTCTGTTCCAGGGGCCCCTGGGATCCAGTTCCT
CGCCTGTTAATGTAAAAAAGCTGAAGGTGTCGGAGCTGAAAGAGGAGCTC
AAGAAGCGACGCCTTTCTGACAAGGGTCTCAAGGCCGAGCTCATGGAGCG
ACTCCAGGCTGCGCTGGACGACGAGGAGGCCGGGGGCCGCCCCGCCATGG
AGCCCGGGAACGGCAGCCTAGACCTGGGCGGGGATTCCGCTGGGCGCTCG
GGAGCAGGCCTCGAGCAGGAGGCCGCGGCCGGCGGCGATGAAGAGGAGGA
AGAAGAGGAAGAGGAGGAGGAAGGAATCTCCGCTCTGGACGGCGACCAGA
TGGAGCTAGGAGAGGAGAACGGGGCCGCGGGGGCGGCCGACTCGGGCCCG
ATGGAGGAGGAGGAGGCCGCCTCGGAAGACGAGAACGGCGACGATCAGGG
TTTCCAGGAAGGGGAAGATGAGCTCGGGGACGAAGAGGAAGGCGCGGGCG
ACGAGAACGGGCACGGGGAGCAGCAGCCTCAACCGCCGGCGACGCAGCAG
CAACAGCCCCAACAGCAGCGCGGGGCCGCCAAGGAGGCCGCGGGGAAGAG
CAGCGGCCCCACCTCGCTGTTCGCGGTGACGGTGGCGCCGCCCGGGGCGA
GGCAGGGCCAGCAGCAGGCGGGAGGTAAGAAGAAGGCGGAAGGCGGCGGA
GGCGGCGGTCGCCCCGGGGCTCCGGCGGCGGGGGACGGCAAAACAGAACA
GAAAGGCGGAGATAAAAAGAGGGGTGTTAAAAGACCACGAGAAGATCATG
GCCGTGGATATTTTGAGTACATTGAAGAGAACAAGTATAGCAGAGCCAAA
TCTCCTCAGCCACCTGTTGAAGAAGAAGATGAACACTTCGATGACACAGT
GGTTTGTCTTGATACTTATAATTGTGATCTACATTTTAAAATATCAAGAG
ATCGTCTCAGTGCTTCTTCCCTTACAATGGAGAGTTTTGCTTTTCTTTGG
GCTGGAGGAAGAGCATCCTATGGTGTGTCAAAAGGCAAAGTGTGTTTTGA
GATGAAGGTTACAGAGAAGATCCCAGTAAGGCATTTATATACAAAAGATA
TTGACATACATGAAGTTCGTATTGGCTGGTCACTAACTACAAGTGGAATG
TTACTTGGTGAAGAAGAATTTTCTTATGGGTATTCTCTAAAAGGAATAAA
AACATGCAACTGTGAGACTGAAGATTATGGAGAAAAGTTTGATGAAAATG
ATGTGATTACATGTTTTGCTAACTTTGAAAGTGATGAAGTAGAACTCTCG
TATGCTAAGAATGGACAAGATCTTGGCGTTGCCTTCAAAATCAGTAAGGA
AGTTCTTGCTGGACGGCCACTGTTCCCGCATGTTCTCTGCCACAACTGTG
CAGTTGAATTTAATTTTGGTCAGAAGGAAAAGCCATATTTTCCAATACCT
GAAGAGTATACTTTCATCCAGAACGTCCCCTTAGAGGATCGAGTTAGAGG
ACCAAAGGGGCCTGAAGAGAAGAAAGATTGTGAAGTTGTGATGATGATTG
GCTTGCCAGGAGCTGGAAAAACTACCTGGGTTACTAAACATGCAGCAGAA
AATCCAGGGAAATATAACATTCTTGGCACAAATACTATTATGGATAAGAT
GATGGTGGCAGGTTTTAAGAAGCAAATGGCAGATACTGGAAAACTGAACA
CACTGTTGCAGAGAGCCCCCCAGTGTCTTGGGAAATTTATTGAGATTGCT
GCCCGAAAGAAGCGAAATTTTATTCTGGATCAGACAAATGTGTCTGCTGC
TGCCCAGAGGAGAAAAATGTGCCTGTTTGCAGGCTTCCAGCGAAAAGCTG
TTGTAGTTTGCCCAAAAGATGAAGACTATAAGCAAAGAACACAGAAGAAA
GCAGAAGTAGAGGGGAAAGACCTACCAGAACATGCGGTCCTCAAAATGAA
AGGAAACTTTACCCTCCCAGAGGTAGCTGAGTGCTTTGATGAAATAACCT
ATGTTGAACTTCAGAAGGAAGAAGCCCAAAAACTCTTGGAGCAATATAAG
GAAGAAAGCAAAAAGGCTCTTCCACCAGAAAAGAAACAGAACACTGGCTC
AAAGAAAAGCAATAAAAATAAGAGTGGCAAGAACCAGTTTAACAGAGGTG
GTGGCCATAGAGGACGTGGAGGACTCAATATGCGTGGTGGAAATTTCAGA
GGAGGAGCCCCTGGGAATCGTGGCGGATATAATAGGAGGGGCAACATGCC
ACAGAGAGGTGGTGGCGGTGGAGGAAGTGGTGGAATCGGCTATCCATACC
CTCGTGCCCCTGTTTTTCCTGGCCGTGGTAGTTACTCAAACAGAGGGAAC
TACAACAGAGGTGGAATGCCCAACAGAGGGAACTACAACCAGAACTTCAG
AGGACGAGGAAACAATCGTGGCTACAAAAATCAATCTCAGGGCTACAACC
AGTGGCAGCAGGGTCAATTCTGGGGTCAGAAGCCATGGAGTCAGCATTAT
CACCAAGGATATTATTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSSSSPVNVKKLKVSELKEEL
KKRRLSDKGLKAELMERLQAALDDEEAGGRPAMEPGNGSLDLGGDSAGRS
GAGLEQEAAAGGDEEEEEEEEEEEGISALDGDQMELGEENGAAGAADSGP
MEEEEAASEDENGDDQGFQEGEDELGDEEEGAGDENGHGEQQPQPPATQQ
QQPQQQRGAAKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGKKKAEGGG
GGGRPGAPAAGDGKTEQKGGDKKRGVKRPREDHGRGYFEYIEENKYSRAK
SPQPPVEEEDEHFDDTVVCLDTYNCDLHFKISRDRLSASSLTMESFAFLW
AGGRASYGVSKGKVCFEMKVTEKIPVRHLYTKDIDIHEVRIGWSLTTSGM
LLGEEEFSYGYSLKGIKTCNCETEDYGEKFDENDVITCFANFESDEVELS
YAKNGQDLGVAFKISKEVLAGRPLFPHVLCHNCAVEFNFGQKEKPYFPIP
EEYTFIQNVPLEDRVRGPKGPEEKKDCEVVMMIGLPGAGKTTWVTKHAAE
NPGKYNILGTNTIMDKMMVAGFKKQMADTGKLNTLLQRAPQCLGKFIEIA
ARKKRNFILDQTNVSAAAQRRKMCLFAGFQRKAVVVCPKDEDYKQRTQKK
AEVEGKDLPEHAVLKMKGNFTLPEVAECFDEITYVELQKEEAQKLLEQYK
EESKKALPPEKKQNTGSKKSNKNKSGKNQFNRGGGHRGRGGLNMRGGNFR
GGAPGNRGGYNRRGNMPQRGGGGGGSGGIGYPYPRAPVFPGRGSYSNRGN
YNRGGMPNRGNYNQNFRGRGNNRGYKNQSQGYNQWQQGQFWGQKPWSQHY
HQGYY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU4087
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial expression with N terminal GST tag. Alanine 459 does not occur in hnRNPU accession number NM_031844.1.