cDNA Clone luciferase

• Plasmid: pcDNA3.1 spacer-CRL (Rluc K230-Q311(end))
• Parent Plasmid: pcDNA3.1
• Expressed: spacer-CRL (Rluc K230-Q311(end))

Sequence of Insert

GGATCCGGTGGCGGAGGGAGCGGTGGCGGAGGGAGCAAACCTGACGTTGT
ACAAATTGTTAGGAATTATAATGCTTATCTACGTGCAAGTGATGATTTAC
CAAAAATGTTTATTGAATCGGACCCAGGATTCTTTTCCAATGCTATTGTT
GAAGGTGCCAAGAAGTTTCCTAATACTGAATTTGTCAAAGTAAAAGGTCT
TCATTTTTCGCAAGAAGATGCACCTGATGAAATGGGAAAATATATCAAAT
CGTTCGTTGAGCGAGTTCTCAAAAATGAACAATAAAGATCC

Amino Acid Sequence

GSGGGGSGGGGSKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIV
EGAKKFPNTEFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36540
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Intermediate clone for receiving different cDNAs to be expressed with C-terminal Renilla luciferase K230-Q311(end) – for use in split luciferase experiments.; Available 5’ sites in MCS : Nhe1/Pme1/AflII/HindIII