cDNA Clone Luciferase (Firefly)

• Plasmid: pcDNA3.1(+) Firefly luciferase (from pGL3(R2.1))
• Parent Plasmid: pcDNA3.1+(neo)
• Expressed: Firefly luciferase
• Genbank: AY487821.1 GI:39653982 REGION: 88..1863

Sequence of Insert

GGATCCATGGCCGATGCTAAGAACATTAAGAAGGGCCCTGCTCCCTTCTA
CCCTCTGGAGGATGGCACCGCTGGCGAGCAGCTGCACAAGGCCATGAAGA
GGTATGCCCTGGTGCCTGGCACCATTGCCTTCACCGATGCCCACATTGAG
GTGGACATCACCTATGCCGAGTACTTCGAGATGTCTGTGCGCCTGGCCGA
GGCCATGAAGAGGTACGGCCTGAACACCAACCACCGCATCGTGGTGTGCT
CTGAGAACTCTCTGCAGTTCTTCATGCCAGTGCTGGGCGCCCTGTTCATC
GGAGTGGCCGTGGCCCCTGCTAACGACATTTACAACGAGCGCGAGCTGCT
GAACAGCATGGGCATTTCTCAGCCTACCGTGGTGTTCGTGTCTAAGAAGG
GCCTGCAGAAGATCCTGAACGTGCAGAAGAAGCTGCCTATCATCCAGAAG
ATCATCATCATGGACTCTAAGACCGACTACCAGGGCTTCCAGAGCATGTA
CACATTCGTGACATCTCATCTGCCTCCTGGCTTCAACGAGTACGACTTCG
TGCCAGAGTCTTTCGACAGGGACAAAACCATTGCCCTGATCATGAACAGC
TCTGGGTCTACCGGCCTGCCTAAGGGCGTGGCCCTGCCTCATCGCACCGC
CTGTGTGCGCTTCTCTCACGCCCGCGACCCTATTTTCGGCAACCAGATCA
TCCCCGACACCGCTATTCTGAGCGTGGTGCCATTCCACCACGGCTTCGGC
ATGTTCACCACCCTGGGCTACCTGATTTGCGGCTTTCGGGTGGTGCTGAT
GTACCGCTTCGAGGAGGAGCTGTTCCTGCGCAGCCTGCAAGACTACAAAA
TTCAGTCTGCCCTGCTGGTGCCAACCCTGTTCAGCTTCTTCGCTAAGAGC
ACCCTGATCGACAAGTACGACCTGTCTAACCTGCACGAGATTGCCTCTGG
CGGCGCCCCACTGTCTAAGGAGGTGGGCGAAGCCGTGGCCAAGCGCTTTC
ATCTGCCAGGCATCCGCCAGGGCTACGGCCTGACCGAGACAACCAGCGCC
ATTCTGATTACCCCAGAGGGCGACGACAAGCCTGGCGCCGTGGGCAAGGT
GGTGCCATTCTTCGAGGCCAAGGTGGTGGACCTGGACACCGGCAAGACCC
TGGGAGTGAACCAGCGCGGCGAGCTGTGTGTGCGCGGCCCTATGATTATG
TCCGGCTACGTGAATAACCCTGAGGCCACAAACGCCCTGATCGACAAGGA
CGGCTGGCTGCACTCTGGCGACATTGCCTACTGGGACGAGGACGAGCACT
TCTTCATCGTGGACCGCCTGAAGTCTCTGATCAAGTACAAGGGCTACCAG
GTGGCCCCAGCCGAGCTGGAGTCTATCCTGCTGCAGCACCCTAACATTTT
CGACGCCGGAGTGGCCGGCCTGCCCGACGACGATGCCGGCGAGCTGCCTG
CCGCCGTCGTCGTGCTGGAACACGGCAAGACCATGACCGAGAAGGAGATC
GTGGACTATGTGGCCAGCCAGGTGACAACCGCCAAGAAGCTGCGCGGCGG
AGTGGTGTTCGTGGACGAGGTGCCCAAGGGCCTGACCGGCAAGCTGGACG
CCCGCAAGATCCGCGAGATCCTGATCAAGGCTAAGAAAGGCGGCAAGATC
GCCGTGAATTCTCACGGCTTCCCTCCCGAGGTGGAGGAGCAGGCCGCCGG
CACCCTGCCCATGAGCTGCGCCCAGGAGAGCGGCATGGATAGACACCCTG
CTGCTTGCGCCAGCGCCAGGATCAACGTCTAACTCGAG

Amino Acid Sequence

MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVD
ITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGV
AVAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKII
IMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSG
STGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMF
TTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTL
IDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAIL
ITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVD
YVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV
NSHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU36508
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee