cDNA Clone GRK3

• Synonyms: ADRBK2, GRK3, BARK2
• Plasmid: pGEX6P-3-GRK3
• Parent Plasmid: pGEX6P3
• Expressed: GST-GRK3
• Genbank: NM_005160.3 GI:148539878
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGACCTGGAGGCTGTGCTGGCCGATGTCAGTTACCTGAT
GGCCATGGAGAAGAGCAAGGCGACCCCGGCCGCCCGCGCCAGCAAGAGGA
TCGTCCTGCCGGAGCCCAGTATCCGGAGTGTGATGCAGAAGTACCTTGCA
GAGAGAAATGAAATAACCTTTGACAAGATTTTCAATCAGAAAATTGGTTT
CTTGCTATTTAAAGATTTTTGTTTGAATGAAATTAATGAAGCTGTACCTC
AGGTGAAGTTTTATGAAGAGATAAAGGAATATGAAAAACTTGATAATGAG
GAAGACCGCCTTTGCAGAAGTCGACAAATTTATGATGCCTACATCATGAA
GGAACTTCTTTCCTGTTCACATCCTTTCTCAAAGCAAGCTGTAGAACACG
TACAAAGTCATTTATCCAAGAAACAAGTGACATCAACTCTTTTTCAGCCA
TACATAGAAGAAATTTGTGAAAGCCTTCGAGGTGACATTTTTCAAAAATT
TATGGAAAGTGACAAGTTCACTAGATTTTGTCAGTGGAAAAACGTTGAAT
TAAATATCCATTTGACCATGAATGAGTTCAGTGTGCATAGGATTATTGGA
CGAGGAGGATTCGGGGAAGTTTATGGTTGCAGGAAAGCAGACACTGGAAA
AATGTATGCAATGAAATGCTTAGATAAGAAGAGGATCAAAATGAAACAAG
GAGAAACATTAGCCTTAAATGAAAGAATCATGTTGTCTCTTGTCAGCACA
GGAGACTGTCCTTTCATTGTATGTATGACCTATGCCTTCCATACCCCAGA
TAAACTCTGCTTCATCCTGGATCTGATGAACGGGGGCGATTTGCACTACC
ACCTTTCACAACACGGTGTGTTCTCTGAGAAGGAGATGCGGTTTTATGCC
ACTGAAATCATTCTGGGTCTGGAACACATGCACAATCGGTTTGTTGTCTA
CAGAGATTTGAAGCCAGCAAATATTCTCTTGGATGAACATGGACACGCAA
GAATATCAGATCTTGGTCTTGCCTGCGATTTTTCCAAAAAGAAGCCTCAT
GCGAGTGTTGGCACCCATGGGTACATGGCTCCCGAGGTGCTGCAGAAGGG
GACGGCCTATGACAGCAGTGCCGACTGGTTCTCCCTGGGCTGCATGCTTT
TCAAACTTCTGAGAGGTCACAGCCCTTTCAGACAACATAAAACCAAAGAC
AAGCATGAAATTGACCGAATGACACTCACCGTGAATGTGGAACTTCCAGA
CACCTTCTCTCCTGAACTGAAGTCCCTTTTGGAGGGCTTGCTTCAGCGAG
ACGTTAGCAAGCGGCTGGGCTGTCACGGAGGCGGCTCACAGGAAGTAAAA
GAGCACAGCTTTTTCAAAGGTGTTGACTGGCAGCATGTCTACTTACAAAA
GTACCCACCACCCTTGATTCCTCCCCGGGGAGAAGTCAATGCTGCTGATG
CCTTTGATATTGGCTCATTTGATGAAGAGGATACCAAAGGGATTAAGCTA
CTTGATTGCGACCAAGAACTCTACAAGAACTTCCCTTTGGTCATCTCTGA
ACGCTGGCAGCAAGAAGTAACGGAAACAGTTTATGAAGCAGTAAATGCAG
ACACAGATAAAATCGAGGCCAGGAAGAGAGCTAAAAATAAGCAACTTGGC
CACGAAGAAGATTACGCTCTGGGGAAGGACTGTATTATGCACGGGTACAT
GCTGAAACTGGGAAACCCATTTCTGACTCAGTGGCAGCGTCGCTATTTTT
ACCTCTTTCCAAATAGACTTGAATGGAGAGGAGAGGGAGAGTCCCGGCAA
AATTTACTGACAATGGAACAGATTCTCTCTGTGGAAGAAACTCAAATTAA
AGACAAAAAATGCATTTTGTTCAGAATAAAAGGAGGGAAACAATTTGTCT
TGCAATGTGAGAGTGATCCAGAGTTTGTGCAGTGGAAGAAAGAGTTGAAC
GAAACCTTCAAGGAGGCCCAGCGGCTATTGCGTCGTGCCCCGAAGTTCCT
CAACAAACCTCGGTCAGGTACTGTGGAGCTCCCAAAGCCATCCCTCTGTC
ACAGAAACAGCAACGGCCTCTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMADLEAVLADVSYLMAMEK
SKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFK
DFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLS
CSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESD
KFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAM
KCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCF
ILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLK
PANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYD
SSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSP
ELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPP
LIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQ
EVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLG
NPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKC
ILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPR
SGTVELPKPSLCHRNSNGL*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36138
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed proteins : 106531 Daltons