cDNA Clone CDKL2

• Synonyms: KKIAMRE, P56
• Plasmid: pGEX6P-CDKL2
• Parent Plasmid: pGEX6P3
• Expressed: GST-CDKL2
• Genbank: NM_003948.3 GI:125987600
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAAAATATGAAAACCTGGGTTTGGTTGGAGAAGGGAGTTA
TGGAATGGTGATGAAGTGTAGGAATAAAGATACTGGAAGAATTGTGGCCA
TAAAGAAGTTCTTAGAAAGTGACGATGACAAAATGGTTAAAAAGATTGCA
ATGCGAGAAATCAAGTTACTAAAGCAACTTAGGCATGAAAACTTGGTGAA
TCTCTTGGAAGTGTGTAAGAAAAAAAAACGATGGTACCTAGTCTTTGAAT
TTGTTGACCACACAATTCTTGATGACTTGGAGCTCTTTCCAAATGGACTA
GACTACCAAGTAGTTCAAAAGTATTTGTTTCAGATTATTAATGGAATTGG
ATTTTGTCACAGTCACAATATCATACACAGAGATATAAAGCCAGAGAATA
TATTAGTCTCCCAGTCTGGCGTTGTCAAGCTATGCGATTTTGGATTTGCG
CGAACATTGGCAGCTCCTGGGGAGGTTTATACTGATTATGTGGCAACCCG
ATGGTACAGAGCTCCAGAACTATTGGTTGGTGATGTCAAGTATGGCAAGG
CTGTTGATGTGTGGGCCATTGGTTGTCTGGTAACTGAAATGTTCATGGGG
GAACCCCTATTTCCTGGAGATTCTGATATTGATCAGCTATATCATATTAT
GATGTGTTTAGGTAATCTAATTCCAAGGCATCAGGAGCTTTTTAATAAAA
ATCCTGTGTTTGCTGGAGTAAGGTTGCCTGAAATCAAGGAAAGAGAACCT
CTTGAAAGACGCTATCCTAAGCTCTCTGAAGTGGTGATAGATTTAGCAAA
GAAATGCTTACATATTGACCCCGACAAAAGACCCTTCTGTGCTGAGCTCC
TACACCATGATTTCTTTCAAATGGATGGATTTGCTGAGAGGTTTTCCCAA
GAACTACAGTTAAAAGTACAGAAAGATGCCAGAAATGTTTCTTTATCTAA
AAAATCCCAAAACAGAAAGAAGGAAAAAGAAAAAGATGATTCCTTAGTTG
AAGAAAGAAAAACACTTGTGGTACAGGATACCAATGCTGATCCCAAAATT
AAGGATTATAAACTATTTAAAATAAAAGGCTCAAAAATTGATGGAGAAAA
AGCTGAAAAAGGCAATAGAGCTTCAAATGCCAGCTGTCTCCATGACAGTA
GGACAAGCCACAACAAAATAGTGCCTTCAACAAGCCTCAAAGACTGCAGC
AATGTCAGCGTGGACCACACAAGGAATCCAAGCGTGGCAATTCCCCCACT
TACACACAATCTTTCTGCAGTTGCTCCCAGCATTAATTCTGGAATGGGGA
CTGAGACTATACCAATTCAGGGTTACAGAGTGGATGAGAAAACTAAGAAG
TGTTCTATTCCATTTGTTAAACCGAACAGACATTCCCCATCAGGCATTTA
TAACATTAATGTGACCACATTAGTATCAGGACCTCCCCTGTCAGATGATT
CAGGGGCTGATTTGCCTCAAATGGAACACCAGCACTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEKYENLGLVGEGSYGMVM
KCRNKDTGRIVAIKKFLESDDDKMVKKIAMREIKLLKQLRHENLVNLLEV
CKKKKRWYLVFEFVDHTILDDLELFPNGLDYQVVQKYLFQIINGIGFCHS
HNIIHRDIKPENILVSQSGVVKLCDFGFARTLAAPGEVYTDYVATRWYRA
PELLVGDVKYGKAVDVWAIGCLVTEMFMGEPLFPGDSDIDQLYHIMMCLG
NLIPRHQELFNKNPVFAGVRLPEIKEREPLERRYPKLSEVVIDLAKKCLH
IDPDKRPFCAELLHHDFFQMDGFAERFSQELQLKVQKDARNVSLSKKSQN
RKKEKEKDDSLVEERKTLVVQDTNADPKIKDYKLFKIKGSKIDGEKAEKG
NRASNASCLHDSRTSHNKIVPSTSLKDCSNVSVDHTRNPSVAIPPLTHNL
SAVAPSINSGMGTETIPIQGYRVDEKTKKCSIPFVKPNRHSPSGIYNINV
TTLVSGPPLSDDSGADLPQMEHQH*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36132
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 82841 Daltons