cDNA Clone MATK

• Synonyms: CHK, CTK, DKFZp434N1212, HHYLTK, HYL
• Plasmid: pGEX6P-3-MATK
• Parent Plasmid: pGEX6P3
• Expressed: GST-MATK
• Genbank: NM_139355.2 GI:111159469
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGCGGGGCGAGGCTCTCTGGTTTCCTGGCGGGCATTTCACGG
CTGTGATTCTGCTGAGGAACTTCCCCGGGTGAGCCCCCGCTTCCTCCGAG
CCTGGCACCCCCCTCCCGTCTCAGCCAGGATGCCAACGAGGCGCTGGGCC
CCGGGCACCCAGTGTATCACCAAATGCGAGCACACCCGCCCCAAGCCAGG
GGAGCTGGCCTTCCGCAAGGGCGACGTGGTCACCATCCTGGAGGCCTGCG
AGAACAAGAGCTGGTACCGCGTCAAGCACCACACCAGTGGACAGGAGGGG
CTGCTGGCAGCTGGGGCGCTGCGGGAGCGGGAGGCCCTCTCCGCAGACCC
CAAGCTCAGCCTCATGCCGTGGTTCCACGGGAAGATCTCGGGCCAGGAGG
CTGTCCAGCAGCTGCAGCCTCCCGAGGATGGGCTGTTCCTGGTGCGGGAG
TCCGCGCGCCACCCCGGCGACTACGTCCTGTGCGTGAGCTTTGGCCGCGA
CGTCATCCACTACCGCGTGCTGCACCGCGACGGCCACCTCACAATCGATG
AGGCCGTGTTCTTCTGCAACCTCATGGACATGGTGGAGCATTACAGCAAG
GACAAGGGCGCTATCTGCACCAAGCTGGTGAGACCAAAGCGGAAACACGG
GACCAAGTCGGCCGAGGAGGAGCTGGCCAGGGCGGGCTGGTTACTGAACC
TGCAGCATTTGACATTGGGAGCACAGATCGGAGAGGGAGAGTTTGGAGCT
GTCCTGCAGGGTGAGTACCTGGGGCAAAAGGTGGCCGTGAAGAATATCAA
GTGTGATGTGACAGCCCAGGCCTTCCTGGACGAGACGGCCGTCATGACGA
AGATGCAACACGAGAACCTGGTGCGTCTCCTGGGCGTGATCCTGCACCAG
GGGCTGTACATTGTCATGGAGCACGTGAGCAAGGGCAACCTGGTGAACTT
TCTGCGGACCCGGGGTCGAGCCCTCGTGAACACCGCTCAGCTCCTGCAGT
TTTCTCTGCACGTGGCCGAGGGCATGGAGTACCTGGAGAGCAAGAAGCTT
GTGCACCGCGACCTGGCCGCCCGCAACATCCTGGTCTCAGAGGACCTGGT
GGCCAAGGTCAGCGACTTTGGCCTGGCCAAAGCCGAGCGGAAGGGGCTAG
ACTCAAGCCGGCTGCCCGTCAAGTGGACGGCGCCCGAGGCTCTCAAACAC
GGGAAGTTCACCAGCAAGTCGGATGTCTGGAGTTTTGGGGTGCTGCTCTG
GGAGGTCTTCTCATATGGACGGGCTCCGTACCCTAAAATGTCACTGAAAG
AGGTGTCGGAGGCCGTGGAGAAGGGGTACCGCATGGAACCCCCCGAGGGC
TGTCCAGGCCCCGTGCACGTCCTCATGAGCAGCTGCTGGGAGGCAGAGCC
CGCCCGCCGGCCACCCTTCCGCAAACTGGCCGAGAAGCTGGCCCGGGAGC
TACGCAGTGCAGGTGCCCCAGCCTCCGTCTCAGGGCAGGACGCCGACGGC
TCCACCTCGCCCCGAAGCCAGGAGCCCTGAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMAGRGSLVSWRAFHGCDSA
EELPRVSPRFLRAWHPPPVSARMPTRRWAPGTQCITKCEHTRPKPGELAF
RKGDVVTILEACENKSWYRVKHHTSGQEGLLAAGALREREALSADPKLSL
MPWFHGKISGQEAVQQLQPPEDGLFLVRESARHPGDYVLCVSFGRDVIHY
RVLHRDGHLTIDEAVFFCNLMDMVEHYSKDKGAICTKLVRPKRKHGTKSA
EEELARAGWLLNLQHLTLGAQIGEGEFGAVLQGEYLGQKVAVKNIKCDVT
AQAFLDETAVMTKMQHENLVRLLGVILHQGLYIVMEHVSKGNLVNFLRTR
GRALVNTAQLLQFSLHVAEGMEYLESKKLVHRDLAARNILVSEDLVAKVS
DFGLAKAERKGLDSSRLPVKWTAPEALKHGKFTSKSDVWSFGVLLWEVFS
YGRAPYPKMSLKEVSEAVEKGYRMEPPEGCPGPVHVLMSSCWEAEPARRP
PFRKLAEKLARELRSAGAPASVSGQDADGSTSPRSQEP*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU36096
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 83289 Daltons