cDNA Clone NDE1-KMLL

• Plasmid: pCMV5-HA NDE1-KMLL
• Parent Plasmid: pCMV5 HA1
• Expressed: HA NDE1-KMLL
• Genbank: GI:108860813*
• Tag: HA
• Species: Human

Sequence of Insert

GGATCCATGGAGGACTCCGGAAAGACTTTCAGCTCCGAGGAGGAAGAAGC
TAACTATTGGAAAGATCTGGCGATGACCTACAAACAGAGGGCAGAAAATA
CGCAAGAGGAACTCCGAGAATTCCAGGAGGGAAGCCGAGAATATGAAGCT
GAATTGGAGACGCAGCTGCAACAAATTGAAACCAGGAACAGAGACCTCCT
GTCCGAAAATAACCGCCTTCGCATGGAGCTGGAAACCATCAAGGAGAAGT
TTGAAGTGCAGCACTCTGAAGGCTACCGGCAGATCTCAGCCTTGGAGGAT
GACCTCGCGCAGACCAAAGCCATTAAAGACCAATTGCAGAAATACATCAG
AGAGCTGGAGCAAGCAAATGACGACCTGGAAAGAGCCAAGCGCGCCACGA
TCATGTCTCTCGAAGACTTTGAGCAGCGCTTGAATCAGGCCATCGAAAGA
AATGCCTTCCTGGAAAGTGAACTTGATGAAAAAGAGAATCTCCTGGAATC
TGTTCAGAGACTGAAGGATGAAGCCAGAGATTTGCGGCAGGAACTGGCCG
TGCAGCAGAAGCAGGAGAAACCCAGGACCCCCATGCCCAGCTCAGTGGAA
GCTGAGAGGACAGACACAGCTGTGCAGGCCACGGGCTCCGTGCCGTCCAC
GCCCATTGCTCACCGAGGACCCAGCTCAAGTTTAAACACACCTGGGAGCT
TCAGACGTGGCCTGGACGACTCCACCGGGGGGACCCCCCTCACACCTGCG
GCCCGGATATCAGCCCTCAACATTGTGGGAGACCTACTGCGGAAAGTCGG
GGCACTGGAGTCCAAACTCGCTTCCTGCCGGAACCTCGTGTACGATCAGT
CCCCAAACCGAACAGGTGGCCCAGCCTCTGGGCGGAGCAGCAAGAACAGA
GATGGCGGGGAGAGACGGCCAAGCAGCACCAGCGTGCCTTTGGGTGATAA
GGGGTTGGGGAAAAGACTGGAATTCGGCAAACCCCCTTCCCACATGTCCT
CTTCACCCTTGCCATCCGCCCAGGGGGTGGTGAAAATGCTGCTCTGAGCG
GCCGC

Amino Acid Sequence

MYPYDVPDYAGSMEDSGKTFSSEEEEANYWKDLAMTYKQRAENTQEELRE
FQEGSREYEAELETQLQQIETRNRDLLSENNRLRMELETIKEKFEVQHSE
GYRQISALEDDLAQTKAIKDQLQKYIRELEQANDDLERAKRATIMSLEDF
EQRLNQAIERNAFLESELDEKENLLESVQRLKDEARDLRQELAVQQKQEK
PRTPMPSSVEAERTDTAVQATGSVPSTPIAHRGPSSSLNTPGSFRRGLDD
STGGTPLTPAARISALNIVGDLLRKVGALESKLASCRNLVYDQSPNRTGG
PASGRSSKNRDGGERRPSSTSVPLGDKGLGKRLEFGKPPSHMSSSPLPSA
QGVVKMLL*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31607
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *No published DNA sequence for this variant as of time of this clone construction. Matches NM_001143979.1 CDS up to nucleotide 952 (residue L317) but differs therafter at C-terminus. ; ; Estimated MW of expressed protein : 40166 Daltons