cDNA Clone PCM1

• Synonyms: *
• Plasmid: pcDNA5-FRT/TO-HA PCM1 ISO 2
• Parent Plasmid: pcDNA5 FRT/TO HA1
• Expressed: HA PCM1
• Genbank: NM_001315507.2
• Tag: HA
• Species: Human

Sequence of Insert

ATGGCCACAGGAGGAGGTCCCTTTGAAGATGGCATGAATGATCAGGATTTACCAAACTGGAGTAATGAGAATGTTGATGACAGGCTCAACAATATGGATTGGGGTGCCCAACAGAAGAAAGCAAATAGATCATCAGAAAAGAATAAGAAAAAGTTTGGTGTAGAAAGTGATAAAAGAGTAACCAATGATATTTCTCCGGAGTCGTCACCAGGAGTTGGAAGGCGAAGAACAAAGACTCCACATACGTTCCCACACAGTAGATACATGAGTCAGATGTCTGTCCCAGAGCAGGCAGAATTAGAGAAACTGAAACAGCGGATAAACTTCAGTGATTTAGATCAGAGAAGCATTGGAAGTGATTCCCAAGGTAGAGCAACAGCTGCTAACAACAAACGTCAGCTTAGTGAAAACCGAAAGCCCTTCAACTTTTTGCCTATGCAGATTAATACTAACAAGAGCAAAGATGCATCTACAAGTCCCCCAAACAGAGAAACGATTGGATCAGCACAGTGTAAAGAGTTGTTTGCTTCTGCTTTAAGTAATGACCTCTTGCAAAACTGTCAGGTGTCTGAAGAAGATGGGAGGGGAGAACCTGCAATGGAGAGCAGCCAGATTGTAAGCAGGCTTGTTCAAATTCGCGATTATATTACTAAAGCTAGTTCCATGCGGGAAGATCTTGTAGAGAAAAATGAGAGATCTGCTAATGTTGAGCGCCTTACTCATCTAATAGATCACCTTAAAGAACAAGAGAAGTCATATATGAAATTTCTTAAAAAAATCCTTGCCAGAGATCCTCAGCAGGAGCCTATGGAAGAGATAGAAAATTTGAAGAAACAACATGATTTATTAAAAAGAATGTTACAACAGCAGGAGCAACTAAGAGCTCTACAGGGACGGCAGGCTGCACTTCTAGCTCTGCAACATAAAGCAGAGCAAGCTATTGCAGTGATGGATGATTCTGTTGTTGCAGAAACTGCAGGTAGCTTATCTGGCGTCAGTATCACATCTGAACTAAATGAAGAATTGAATGACTTAATTCAGCGTTTTCATAATCAGCTTCGTGATTCTCAGCCTCCAGCTGTTCCAGACAATAGAAGACAGGCAGAAAGTCTTTCATTAACTAGGGAGGTTTCCCAGAGCAGGAAACCATCAGCTTCAGAACGTTTACCTGATGAGAAAGTCGAACTTTTTAGCAAAATGAGAGTGCTACAGGAAAAGAAACAAAAAATGGACAAATTGCTTGGAGAACTTCATACACTTCGAGATCAGCATCTTAACAATTCATCATCCTCTCCACAAAGGAGTGTCGATCAGAGAAGTACTTCAGCTCCCTCTGCTTCTGTAGGCTTGGCACCGGTTGTCAATGGAGAATCCAATAGCCTCACATCATCTGTTCCTTATCCTACTGCTTCTCTAGTATCTCAGAATGAGAGTGAAAACGAAGGCCACCTCAATCCATCTGAAAAACTCCAGAAGTTAAATGAAGTTCGAAAGAGATTGAATGAGCTAAGAGAATTAGTTCATTATTATGAACAAACGTCAGACATGATGACAGATGCTGTGAATGAAAACAGGAAAGATGAAGAAACTGAAGAGTCAGAATATGATTCTGAGCATGAAAATTCCGAGCCTGTTACTAACATTCGAAATCCACAAGTAGCTTCCACTTGGAATGAAGTAAATAGTCATAGTAATGCACAGTGTGTTTCTAATAATAGAGATGGGCGAACAGTTAATTCTAATTGTGAAATTAACAACAGATCTGCTGCCAACATAAGGGCTCTAAACGTGCCTCCTTCTTTAGATTGTCGATATAATAGAGAAGGGGAACAGGAGATTCATGTTGCACAAGGTGAAGATGATGAGGAGGAGGAGGAAGAAGCAGAAGAGGAGGGAGTCAGTGGAGCTTCATTATCTAGTCACAGGAGCAGTCTGGTTGATGAGCATCCAGAAGATGCTGAATTTGAACAGAAGATCAACCGACTTATGGCTGCAAAACAGAAACTTAGACAGTTACAAGATCTTGTTGCTATGGTACAGGATGATGATGCAGCTCAAGGAGTTATCTCTGCCAGTGCATCAAATTTGGATGATTTCTACCCAGCAGAAGAAGACACCAAGCAAAATTCAAATAACACTAGAGGAAATGCCAATAAAACACAGAAAGATACTGGAGTAAATGAAAAGGCAAGAGAGAAATTTTATGAGGCTAAACTACAGCAGCAACAGAGAGAGCTAAAACAATTGCAGGAAGAAAGAAAGAAACTGATTGACATTCAGGAGAAAATTCAAGCATTGCAAACGGCATGCCCTGACTTACAGCTGTCAGCTGCTAGTGTGGGTAACTGTCCTACCAAAAAATATATGCCAGCTGTTACTTCAACCCCAACTGTTAATCAACACGAGACCAGTACAAGCAAATCTGTTTTTGAGCCTGAAGATTCTTCAATAGTAGATAATGAGTTGTGGTCAGAAATGAGAAGACATGAAATGTTGAGGGAGGAGCTGCGACAGAGAAGAAAGCAGCTTGAAGCTCTGATGGCTGAACATCAGAGGAGGCAAGGTCTAGCTGAAACTGCATCTCCAGTGGCTGTGTCATTGAGAAGTGATGGATCTGAGAACCTATGTACTCCTCAGCAAAGTAGAACAGAAAAAACGATGGCAACTTGGGGAGGGTCTACCCAGTGTGCACTAGATGAAGAAGGAGATGAAGACGGTTACCTTTCTGAAGGAATTGTTCGGACAGATGAAGAGGAGGAAGAAGAGCAAGATGCCAGTTCCAATGATAACTTTTCTGTGTGTCCTTCTAACAGTGTGAATCATAACTCCTACAATGGAAAGGAAACTAAAAATAGGTGGAAGAACAATTGCCCTTTTTCGGCAGATGAAAATTATCGTCCTTTAGCCAAGACAAGGCAACAGAATATCAGCATGCAACGGCAAGAAAACCTTCGTTGGGTGTCAGAGCTCTCTTACGTAGAAGAGAAAGAACAATGGCAAGAACAAATCAATCAGCTAAAGAAACAGCTTGATTTTAGTGTCAGTATTTGTCAGACTTTGATGCAAGACCAGCAGACTCTATCTTGTCTGCTACAAACTCTTCTCACGGGTCCTTACAGTGTTATGCCCAGCAATGTTGCATCTCCTCAAGTACACTTCATAATGCACCAGTTGAACCAGTGCTATACTCAGCTAACATGGCAACAGAATAATGTTCAGAGGTTGAAACAAATGCTAAATGAACTTATGCGCCAGCAAAATCAGCATCCAGAAAAACCTGGAGGCAAGGAAAGAGGCAGTAGTGCATCGCACCCTCCTTCTCCCAGTTTATTTTGTCCTTTCAGCTTTCCAACACAGCCTGTAAATCTCTTCAATATACCTGGATTTACTAACTTTTCATCATTTGCACCAGGTATGAATTTCAGCCCTTTATTTCCTTCTAATTTTGGAGATTTTTCTCAGAATATCTCTACACCCAGTGAACAGCAGCAACCCTTAGCCCAGAATTCTTCAGGAAAAACAGAATATATGGCTTTTCCAAAACCTTTTGAAAGCAGTTCCTCTATTGGAGCAGAGAAACCAAGGAATAAAAAACTGCCTGAAGAGGAGGTGGAAAGCAGTAGGACACCATGGTTATATGAACAAGAAGGTGAAGTAGAGAAACCATTTATCAAGACTGGATTTTCAGTGTCTGTAGAAAAATCTACAAGTAGTAACCGCAAAAATCAATTAGATACAAACGGAAGAAGACGCCAGTTTGATGAAGAATCACTGGAAAGCTTTAGCAGTATGCCTGATCCAGTAGATCCAACAACAGTGACTAAAACATTCAAGACAAGAAAAGCGTCTGCACAGGCCAGCCTGGCATCTAAAGATAAAACTCCCAAGTCAAAAAGTAAGAAGAGGAATTCTACTCAGCTGAAAAGCAGAGTTAAAAACATCAGGTATGAAAGTGCCAGTATGTCTAGCACATGTGAACCTTGCAAAAGTAGGAACAGACATTCAGCCCAGACTGAAGAGCCTGTTCAAGCAAAAGTATTCAGCAGAAAGAATCATGAGCAACTGGAAAAAATAATAAAATGTAATAGGTCTACAGAAATATCTTCAGAAACTGGGAGTGATTTTTCCATGTTTGAAGCTTTGCGAGATACTATTTATTCTGAAGTAGCTACATTAATTTCTCAAAATGAATCTCGTCCACATTTTCTTATTGAACTCTTCCATGAGCTGCAGCTACTAAACACAGACTACTTGAGACAGAGGGCTTTATATGCATTGCAGGACATAGTATCCAGACATATTTCTGAGAGCCATGAAAAAGGAGAAAATGTAAAGTCAGTAAACTCTGGTACTTGGATAGCATCAAACTCAGAACTTACTCCTAGTGAGAGCCTTGCTACTACTGATGATGAAACTTTTGAGAAGAACTTTGAAAGAGAAACCCATAAAATAAGTGAGCAAAATGATGCTGATAATGCTAGTGTCCTGTCTGTATCATCAAATTTTGAGCCTTTTGCAACAGATGATCTAGGTAACACCGTGATTCACTTAGATCAAGCATTAGCCAGAATGAGAGAATATGAGCGTATGAAGACTGAGGCTGAAAGTAACTCAAATATGAGATGCACCTGCAGGATTATTGAGGATGGAGATGGTGCTGGTGCAGGTACTACAGTTAATAATTTAGAAGAAACTCCCGTTATTGAAAATCGTAGTTCACAACAACCTGTAAGTGAAGTTTCTACCATCCCATGTCCTAGAATTGATACTCAGCAGCTGGACCGGCAAATTAAAGCAATTATGAAAGAAGTCATTCCTTTTTTGAAGGAGCACATGGATGAAGTATGCTCCTCGCAGCTTCTAACTTCAGTAAGGCGCATGGTTTTGACCCTTACCCAGCAAAATGATGAGAGCAAAGAGTTTGTAAAGTTCTTTCATAAACAACTTGGAAGTATATTACAGGATTCACTGGCAAAATTTGCTGGCAGAAAACTGAAAGACTGTGGAGAAGATCTTCTTGTAGAGATATCTGAAGTGTTGTTCAATGAATTGGCTTTCTTTAAGCTTATGCAAGATTTGGATAATAATAGTATAACTGTTAAACAGAGATGCAAAAGGAAAATAGAAGCAACTGGAGTGATACAATCTTGTGCCAAAGAGGCTAAAAGGATTCTTGAAGATCATGGCTCACCTGCTGGAGAGATTGATGATGAAGACAAAGACAAGGATGAAACTGAAACAGTTAAGCAGACTCAAACATCTGAGGTGTATGATGGTCCCAAAAATGTAAGATCTGATATTTCTGATCAAGAGGAAGATGAAGAAAGTGAAGGATGTCCAGTGTCTATTAATTTGTCTAAAGCTGAAACTCAGGCTTTAACTAATTATGGAAGTGGAGAAGATGAAAATGAGGATGAAGAAATGGAAGAATTTGAAGAAGGCCCTGTGGATGTCCAGACTTCCCTCCAGGCTAACACTGAAGCTACTGAAGAAAATGAACATGATGAACAGGTCCTACAACGTGACTTTAAAAAGACAGCAGAAAGCAAAAATGTCCCATTGGAACGAGAAGCCACTAGTAAAAATGACCAAAATAACTGTCCTGTGAAACCCTGTTACCTCAATATCTTGGAAGATGAGCAACCTTTAAATAGTGCTGCCCATAAGGAGTCACCTCCTACTGTTGATTCAACTCAACAGCCTAACCCTTTGCCGTTACGTTTACCTGAAATGGAACCCTTAGTGCCTAGAGTCAAAGAAGTTAAATCTGCTCAGGAAACTCCTGAAAGCTCTCTGGCTGGAAGTCCTGATACTGAATCTCCAGTGTTAGTGAATGACTATGAAGCAGAATCTGGTAATATAAGTCAAAAGTCTGATGAAGAAGATTTTGTAAAAGTTGAAGATTTACCACTGAAACTGACAATATATTCAGAGGCAGATCTAAGAAAGAAAATGGTAGAAGAAGAACAGAAAAACCATTTATCTGGTGAAATATGTGAAATGCAGACCGAAGAATTAGCTGGAAATTCTGAGACACTAAAAGAACCTGAAACGGTGGGAGCCCAGAGTATATGA

Amino Acid Sequence

MYPYDVPDYAGSMATGGGPFEDGMNDQDLPNWSNENVDDRLNNMDWGAQQ
KKANRSSEKNKKKFGVESDKRVTNDISPESSPGVGRRRTKTPHTFPHSRY
MSQMSVPEQAELEKLKQRINFSDLDQRSIGSDSQGRATAANNKRQLSENR
KPFNFLPMQINTNKSKDASTSPPNRETIGSAQCKELFASALSNDLLQNCQ
VSEEDGRGEPAMESSQIVSRLVQIRDYITKASSMREDLVEKNERSANVER
LTHLIDHLKEQEKSYMKFLKKILARDPQQEPMEEIENLKKQHDLLKRMLQ
QQEQLRALQGRQAALLALQHKAEQAIAVMDDSVVAETAGSLSGVSITSEL
NEELNDLIQRFHNQLRDSQPPAVPDNRRQAESLSLTREVSQSRKPSASER
LPDEKVELFSKMRVLQEKKQKMDKLLGELHTLRDQHLNNSSSSPQRSVDQ
RSTSAPSASVGLAPVVNGESNSLTSSVPYPTASLVSQNESENEGHLNPSE
KLQKLNEVRKRLNELRELVHYYEQTSDMMTDAVNENRKDEETEESEYDSE
HENSEPVTNIRNPQVASTWNEVNSHSNAQCVSNNRDGRTVNSNCEINNRS
AANIRALNVPPSLDCRYNREGEQEIHVAQGEDDEEEEEEAEEEGVSGASL
SSHRSSLVDEHPEDAEFEQKINRLMAAKQKLRQLQDLVAMVQDDDAAQGV
ISASASNLDDFYPAEEDTKQNSNNTRGNANKTQKDTGVNEKAREKFYEAK
LQQQQRELKQLQEERKKLIDIQEKIQALQTACPDLQLSAASVGNCPTKKY
MPAVTSTPTVNQHETSTSKSVFEPEDSSIVDNELWSEMRRHEMLREELRQ
RRKQLEALMAEHQRRQGLAETASPVAVSLRSDGSENLCTPQQSRTEKTMA
TWGGSTQCALDEEGDEDGYLSEGIVRTDEEEEEEQDASSNDNFSVCPSNS
VNHNSYNGKETKNRWKNNCPFSADENYRPLAKTRQQNISMQRQENLRWVS
ELSYVEEKEQWQEQINQLKKQLDFSVSICQTLMQDQQTLSCLLQTLLTGP
YSVMPSNVASPQVHFIMHQLNQCYTQLTWQQNNVQRLKQMLNELMRQQNQ
HPEKPGGKERGSSASHPPSPSLFCPFSFPTQPVNLFNIPGFTNFSSFAPG
MNFSPLFPSNFGDFSQNISTPSEQQQPLAQNSSGKTEYMAFPKPFESSSS
IGAEKPRNKKLPEEEVESSRTPWLYEQEGEVEKPFIKTGFSVSVEKSTSS
NRKNQLDTNGRRRQFDEESLESFSSMPDPVDPTTVTKTFKTRKASAQASL
ASKDKTPKSKSKKRNSTQLKSRVKNIRYESASMSSTCEPCKSRNRHSAQT
EEPVQAKVFSRKNHEQLEKIIKCNRSTEISSETGSDFSMFEALRDTIYSE
VATLISQNESRPHFLIELFHELQLLNTDYLRQRALYALQDIVSRHISESH
EKGENVKSVNSGTWIASNSELTPSESLATTDDETFEKNFERETHKISEQN
DADNASVLSVSSNFEPFATDDLGNTVIHLDQALARMREYERMKTEAESNS
NMRCTCRIIEDGDGAGAGTTVNNLEETPVIENRSSQQPVSEVSTIPCPRI
DTQQLDRQIKAIMKEVIPFLKEHMDEVCSSQLLTSVRRMVLTLTQQNDES
KEFVKFFHKQLGSILQDSLAKFAGRKLKDCGEDLLVEISEVLFNELAFFK
LMQDLDNNSITVKQRCKRKIEATGVIQSCAKEAKRILEDHGSPAGEIDDE
DKDKDETETVKQTQTSEVYDGPKNVRSDISDQEEDEESEGCPVSINLSKA
ETQALTNYGSGEDENEDEEMEEFEEGPVDVQTSLQANTEATEENEHDEQV
LQRDFKKTAESKNVPLEREATSKNDQNNCPVKPCYLNILEDEQPLNSAAH
KESPPTVDSTQQPNPLPLRLPEMEPLVPRVKEVKSAQETPESSLAGSPDT
ESPVLVNDYEAESGNISQKSDEEDFVKVEDLPLKLTIYSEADLRKKMVEE
EQKNHLSGEICEMQTEELAGNSETLKEPETVGAQSI*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU31427
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: *Sequence differences compared with NM_006197.3 CDS: ; a1789g (M597V), c2352t (silent) - present in EST and many other published RNA seqs.
• Low Temp: No