cDNA Clone ATG10

• Plasmid: pGEX6P-2-ATG10
• Parent Plasmid: pGEX6P2
• Expressed: GST-ATG10
• Genbank: NM_031482.4 GI:196162715
• Tag: GST
• Species: Human

Sequence of Insert

GGATCCATGGAAGAAGATGAGTTCATTGGAGAAAAAACATTCCAACGTTA
TTGTGCAGAATTCATTAAACATTCACAACAGATAGGTGATAGTTGGGAAT
GGAGACCATCAAAGGACTGTTCTGATGGCTACATGTGCAAAATACACTTT
CAAATTAAGAATGGGTCTGTGATGTCACATCTAGGAGCATCTACCCATGG
ACAGACATGTCTTCCCATGGAGGAGGCTTTCGAGCTACCCTTGGATGATT
GTGAAGTGATTGAAACTGCAGCAGCGTCCGAAGTGATTAAATATGAGTAT
CATGTCTTATATTCCTGTAGCTACCAAGTGCCTGTACTTTACTTTAGGGC
AAGCTTTTTAGATGGGAGACCTTTAACTCTGAAGGACATATGGGAAGGAG
TTCATGAGTGCTATAAGATGCGACTGCTACAGGGACCATGGGACACTATT
ACGCAACAGGAACATCCAATACTTGGGCAACCCTTTTTTGTACTTCATCC
CTGCAAGACGAATGAATTCATGACTCCTGTATTAAAGAATTCTCAGAAAA
TCAATAAGAATGTCAACTATATCACATCATGGCTGAGCATTGTAGGGCCA
GTTGTTGGGCTGAATCTACCTCTGAGTTATGCCAAAGCAACGTCTCAGGA
TGAACGAAATGTCCCTTAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSMEEDEFIGEKTFQRYCAEF
IKHSQQIGDSWEWRPSKDCSDGYMCKIHFQIKNGSVMSHLGASTHGQTCL
PMEEAFELPLDDCEVIETAAASEVIKYEYHVLYSCSYQVPVLYFRASFLD
GRPLTLKDIWEGVHECYKMRLLQGPWDTITQQEHPILGQPFFVLHPCKTN
EFMTPVLKNSQKINKNVNYITSWLSIVGPVVGLNLPLSYAKATSQDERNV
P*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31144
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 52101 Daltons