cDNA Clone Kif1b alpha

• Plasmid: pGEX6P-2-Kif1b alpha
• Parent Plasmid: pGEX6P2
• Expressed: GST-Kif1b alpha
• Genbank: NM_183416.3 GI:189339260
• Tag: GST
• Species: Human

Sequence of Insert

GCGGCCGCAATGTCGGGAGCCTCAGTGAAGGTGGCTGTCCGGGTAAGGCC
CTTCAATTCTCGAGAGACCAGCAAGGAATCCAAATGCATCATTCAGATGC
AAGGCAACTCGACCAGTATTATTAACCCAAAGAATCCAAAGGAAGCTCCA
AAGTCCTTCAGCTTCGACTATTCCTACTGGTCTCATACCTCACCCGAAGA
TCCCTGTTTTGCATCTCAAAACCGTGTGTACAATGACATTGGCAAGGAAA
TGCTCTTACACGCCTTTGAGGGATATAATGTCTGTATTTTTGCCTATGGG
CAGACTGGTGCTGGAAAATCTTATACAATGATGGGTAAACAAGAAGAAAG
CCAGGCTGGCATCATTCCACAGTTATGTGAAGAACTTTTTGAGAAAATCA
ATGACAACTGTAATGAAGAAATGTCTTACTCTGTAGAGGTGAGCTACATG
GAAATTTACTGTGAAAGAGTACGAGATTTGCTGAATCCAAAAAACAAGGG
TAATTTGCGTGTGCGTGAACACCCACTTCTTGGACCCTATGTGGAGGATC
TGTCCAAGTTGGCAGTTACTTCCTACACAGACATTGCTGACCTCATGGAT
GCTGGGAACAAAGCCAGGACAGTGGCAGCTACAAACATGAATGAAACAAG
TAGCCGTTCCCACGCTGTGTTTACGATTGTTTTCACCCAGAAGAAACACG
ATAATGAGACCAACCTTTCCACTGAGAAGGTCAGTAAAATCAGCTTGGTG
GATCTAGCAGGAAGTGAACGAGCTGATTCAACTGGTGCCAAAGGGACTCG
ATTAAAGGAAGGAGCAAATATTAATAAGTCTCTTACAACTTTGGGCAAAG
TCATTTCAGCCTTGGCCGAGGTGAGTAAAAAGAAGAAGAAAACAGATTTT
ATTCCCTACAGGGATTCTGTACTTACTTGGCTCCTTCGAGAAAATTTAGG
TGGCAATTCTCGGACTGCAATGGTTGCTGCTCTGAGCCCCGCGGATATCA
ACTACGATGAGACTTTGAGCACTCTGAGATATGCAGATCGTGCAAAACAA
ATTAAATGCAATGCTGTTATCAATGAGGACCCCAATGCCAAACTGGTTCG
TGAATTAAAGGAGGAGGTGACACGGCTGAAGGACCTTCTTCGTGCTCAGG
GCCTGGGAGATATTATTGATACATCCATGGGGTCCCTCACTTCATCCCCA
TCTTCCTGCTCACTCAGTAGTCAGGTGGGCTTGACGTCTGTGACCAGTAT
TCAAGAGAGGATCATGTCTACACCTGGAGGAGAGGAAGCTATTGAACGTT
TAAAGGAATCAGAGAAGATCATTGCTGAGTTGAATGAAACTTGGGAAGAG
AAGCTTCGTAAAACAGAGGCCATCAGAATGGAGAGAGAGGCTTTGTTGGC
TGAGATGGGAGTTGCCATTCGGGAAGATGGAGGAACCCTAGGGGTTTTCT
CACCTAAAAAGACCCCACATCTTGTTAACCTCAATGAAGACCCACTAATG
TCTGAGTGCCTACTTTATTACATCAAAGATGGAATTACAAGGGTTGGCCA
AGCAGATGCTGAGCGGCGCCAGGACATAGTGCTGAGCGGGGCTCACATTA
AAGAAGAGCATTGTATCTTCCGGAGTGAGAGAAGCAACAGCGGGGAAGTT
ATCGTGACCTTAGAGCCCTGTGAGCGCTCAGAAACCTACGTAAATGGCAA
GAGGGTGTCCCAGCCTGTTCAGCTGCGCTCAGGAAACCGTATCATCATGG
GTAAAAACCATGTTTTCCGCTTTAACCACCCGGAACAAGCACGAGCTGAG
CGAGAGAAGACTCCTTCTGCTGAGACCCCCTCTGAGCCTGTGGACTGGAC
ATTTGCCCAGAGGGAGCTTCTGGAAAAACAAGGAATTGATATGAAACAAG
AGATGGAGAAAAGGCTACAGGAAATGGAGATCTTATACAAAAAGGAGAAG
GAAGAAGCAGATCTTCTTTTGGAGCAGCAGAGACTGGACGCGGATTCTGA
TAGCGGGGACGATTCTGACAAGAGGTCGTGTGAAGAGAGCTGGAAACTGA
TTACTTCTCTGAGAGAAAAGCTACCTCCCAGCAAGTTGCAAACCATTGTT
AAAAAATGTGGCCTCCCAAGCAGTGGGAAGAAACGTGAACCAATTAAAAT
GTATCAGATACCCCAAAGAAGGCGCTTGAGTAAAGATTCCAAGTGGGTCA
CAATCTCAGATCTTAAAATTCAGGCTGTCAAAGAGATTTGCTATGAGGTT
GCTCTCAATGACTTCAGGCACAGTCGGCAGGAGATTGAAGCCCTGGCCAT
TGTCAAGATGAAGGAGCTTTGTGCCATGTATGGCAAGAAAGACCCCAATG
AGCGGGACTCCTGGAGGGCAGTGGCCAGGGACGTCTGGGATACCGTCGGT
GTTGGGGATGAGAAGATCGAAGACGTCATGGCCACTGGGAAAGGCAGCAC
TGATGTAGATGACCTCAAGGTTCATATAGACAAGCTGGAAGATATTTTGC
AAGAAGTCAAAAAGCAAAATAACATGAAAGACGAGGAGATAAAAGTCTTA
AGAAATAAAATGCTCAAAATGGAAAAAGTCTTGCCACTGATCGGATCTCA
GGAACAGAAAAGCCCAGGAAGCCACAAAGCAAAGGAGCCTGTTGGTGCTG
GTGTTAGTAGCACCTCTGAGAATAATGTAAGTAAAGGAGACAATGGAGAA
CTTGCAAAAGAAGAACGTGTTTCCCAGCTGATGAATGGGGATCCAGCTTT
TAGACGTGGACGTCTGCGCTGGATGAGGCAAGAGCAAATTCGGTTTAAGA
ACTTGCAACAGCAGGAGATAACAAAGCAGCTTCGTCGGCAGAATGTACCT
CATAGGTTCATCCCTCCTGAGAACCGGAAGCCCCGCTTCCCCTTTAAGAG
CAACCCTAAACACAGAAACTCTTGGAGTCCTGGGACACATATCATCATAA
CAGAAGATGAGGTTATAGAGCTTAGGATTCCAAAAGACGATGAAGCAAGG
AAAGGGAATAAAGAAGAGAGCCAAGAAAAAGGGGGTAAAGGAGCTTTTAA
GGATCCCCAGTTTCCATGGGGCTCTCAAGGAATGAGAAGTCAAGATCACA
TCCAAGTTAGCAAGCAGCACATTAATAATCAGCAACAGCCACCTCAACTA
CGTTGGAGAAGCAATTCTCTCAATAATGGCCAGCCGAAAAGTACGCGCTG
CCAGGCATCTGCCTCCGCGGAGTCATTAAACTCCCACAGTGGTCACCCCA
CTGCTGATGTACAGACTTTCCAGGCAAAGCGCCATATTCATCAACACCGT
CAGTCTTACTGTAATTATAACACTGGAGGTCAGTTAGAGGGCAATGCAGC
CACTTCCTATCAGAAGCAGACTGACAAACCCAGCCACTGTAGCCAGTTTG
TGACACCTCCGCGGATGAGGAGACAGTTCTCAGCACCCAATCTCAAAGCT
GGTCGAGAAACCACAGTATAAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPEIPGSTRAAAMSGASVKV
AVRVRPFNSRETSKESKCIIQMQGNSTSIINPKNPKEAPKSFSFDYSYWS
HTSPEDPCFASQNRVYNDIGKEMLLHAFEGYNVCIFAYGQTGAGKSYTMM
GKQEESQAGIIPQLCEELFEKINDNCNEEMSYSVEVSYMEIYCERVRDLL
NPKNKGNLRVREHPLLGPYVEDLSKLAVTSYTDIADLMDAGNKARTVAAT
NMNETSSRSHAVFTIVFTQKKHDNETNLSTEKVSKISLVDLAGSERADST
GAKGTRLKEGANINKSLTTLGKVISALAEVSKKKKKTDFIPYRDSVLTWL
LRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIKCNAVINEDP
NAKLVRELKEEVTRLKDLLRAQGLGDIIDTSMGSLTSSPSSCSLSSQVGL
TSVTSIQERIMSTPGGEEAIERLKESEKIIAELNETWEEKLRKTEAIRME
REALLAEMGVAIREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDG
ITRVGQADAERRQDIVLSGAHIKEEHCIFRSERSNSGEVIVTLEPCERSE
TYVNGKRVSQPVQLRSGNRIIMGKNHVFRFNHPEQARAEREKTPSAETPS
EPVDWTFAQRELLEKQGIDMKQEMEKRLQEMEILYKKEKEEADLLLEQQR
LDADSDSGDDSDKRSCEESWKLITSLREKLPPSKLQTIVKKCGLPSSGKK
REPIKMYQIPQRRRLSKDSKWVTISDLKIQAVKEICYEVALNDFRHSRQE
IEALAIVKMKELCAMYGKKDPNERDSWRAVARDVWDTVGVGDEKIEDVMA
TGKGSTDVDDLKVHIDKLEDILQEVKKQNNMKDEEIKVLRNKMLKMEKVL
PLIGSQEQKSPGSHKAKEPVGAGVSSTSENNVSKGDNGELAKEERVSQLM
NGDPAFRRGRLRWMRQEQIRFKNLQQQEITKQLRRQNVPHRFIPPENRKP
RFPFKSNPKHRNSWSPGTHIIITEDEVIELRIPKDDEARKGNKEESQEKG
GKGAFKDPQFPWGSQGMRSQDHIQVSKQHINNQQQPPQLRWRSNSLNNGQ
PKSTRCQASASAESLNSHSGHPTADVQTFQAKRHIHQHRQSYCNYNTGGQ
LEGNAATSYQKQTDKPSHCSQFVTPPRMRRQFSAPNLKAGRETTV*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU31025
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion ; ; Estimated MW of expressed protein : 158234 Daltons