cDNA Clone FPA (Arabidopsis)

• Plasmid: pcDNA5-FRT/TO-GFP FPA (N.B. Q888R)
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP FPA
• Genbank: NP_001078046.1 GI:145331109*
• Tag: GFP
• Species: Arabidopsis thaliana

Sequence of Insert

GGATCCATGGCGTTATCTATGAAGCCATTCAGAGCCGATGATTCCGGTTT
CCAGTCAAACAATCTTTGGGTCGGTAGCCTAACGCCGGAGACGACAGAGT
CAGATCTGACCGAGTTGTTTGGAAGATACGGCGATATTGATAGAATCACG
GTGTATTCTTCACGAGGCTTTGCGTTTATATACTACAGACATGTGGAGGA
AGCAGTCGCAGCCAAAGAGGCTCTTCAAGGAGCAAATTTGAATGGAAGTC
AAATTAAGATCGAATACGCACGACCGGCAAAACCTTGTAAGAGTCTATGG
GTGGGTGGAATCGGCCCTAATGTCTCCAAGGATGACCTGGAGGAAGAGTT
CAGCAAGTTTGGGAAAATCGAGGATTTTAGGTTTCTCAGAGAACGCAAGA
CAGCTTTCATTGATTATTATGAGATGGATGATGCTTTACAGGCTAAGAGC
ATGAATGGAAAGCCTATGGGTGGTAGCTTTTTGCGTGTTGATTTTCTCCG
GTCACAAGCGCCAAAAAAAGAACAATGGGCTGGCTCTTACGATAACAGAA
ATGGCAATATGAATCATAAACCGCAGTATCCTCACTCATATGAAGACTTT
AAAGGAGATGTCCAGCCAAGTAAGGTTCTGTGGATTGGGTTCCCTCCTAC
TGCTACACAATGCAATGATGAGCAAATTCTGCACAATGCGATGATACTCT
TTGGTGAGATCGAGAGGGTAAAAAGTTACCCATCAAGGAATTTTGCACTT
GTGGAGTTTAGGAGCGCGGAGGAAGCTCGCCAATGCAAGGAAGGCCTACA
GGGGAGGTTATTCAATAATCCTAGAATCAAAATTATGTACTCAAACGATG
AGTTGCCTCCTGAGCAAGACGATACTAGTTTTTACTCTGGTATGAAACGG
TCAAGGACAGATATGTTCAATAATGATCCTTCATTTGTATCTTCTCCTCA
TTCTACTGGAATTCCTGGGTCTATGAGGCCCCTCAGAGGTACAAATGAGC
GTTCATATAATGGTGCAGAATACAATGACGTTGTTGGTAAGGAGCCAAAC
TGGAGGAGGCCATCTGCAAATGGAACTGGAATACTCCCATCTCCAACAGG
ACCTGGAATCCTCCCATCTCCTGCACAAGGTACGAGGCGCCCTATGAGGT
CAAACCCCGATTCTTGGGAAGGATATGATCCTGCTCAGTTGGTCAGAGAA
AGTAAACGAACCAGAAGAGATGGATCAGTGGACGGTTTTACTCCAATGGG
TGTCGATGAGAGGTCATTTGGTCGAGGTTCAGTTGCTGCTAGACCTATCC
GTGGCCCCCCTGATTCTGATCACATATGGAGAGGAATGATTGCCAAGGGT
GGAACTCCCGTCTGTTGTGCTCGTTGTGTACCTATGGGAAAGGGGATTGA
AACTAAACTGCCTGAGGTCGTCAATTGTTCAGCAAGAACTGATTTGAATA
TGCTCGCTAAACATTACGCCGTTGCCATTGGATGTGAGATCGTTTTTTTC
GTACCAGACAGGGAAGAAGATTTTGCGTCTTACACTGAATTTCTCCGGTA
CCTTAGCTCAAAAGATCGGGCGGGTGTTGCCAAATTAGATGATGGTACAA
CTTTATTCTTGGTGCCTCCATCAGATTTCTTAACTGATGTACTCCAAGTG
ACCCGTCAAGAACGGCTATATGGTGTTGTTCTCAAGTTACCCCCGCCAGC
CGTTCCTGTTACAGCATCATACAGACAAGAATCTCAGTCCAATCCTCTGC
ATTATATGGATCAAGCCCGGGATTCACCTGCCAATGCTAGTCACAGTTTA
TATCCTCCTAGGGAAAATTACATTAGGGGTGCACCAGAACATTTGACAGC
TGCTTCAAAACCATCTGTTAGCGAGCCTCTCAGAATACCTAATAATGCAG
CGCCTCAAGCTGGGGTTAGTTTAACTCCGGAGCTTTTAGCCACTCTGGCA
TCTATTCTCCCTGCAACTTCTCAACCTGCTGCCCCTGAGAGTCACCAACC
TATGTCAGGACCTTCAACAGTTGTTTCCACAGCACATCAGTCCAATGGAC
TGTACAATGGAGAAGCACCGTCTCAAGCTTGGAAAAGAGGTCCACAAACA
GTTCATGATGCGTCAAATCAGTCATTCCAACAATACGGAAATCAGTACAC
TCCAGCTGGGCAACTACCTCCTCCTCCTTCGCGTTACCCTCCAGCTTCAA
ACAACCCCAACTACACTAGTGGAATGGTCCATGGCAACATGCAATACCAG
AGCCAATCTGTTAACATGCCTCAGCTGTCTCCGTTACCAAATATGCCTCA
TAATAATTATTCCATGTACACTCAGGGTTCGTCAAATCATCCTGTTTCTC
AGCCCATGGTCCAGCAATACCAACCAGAAGCGTCCATGCCAAACCAAAAC
TATGGTCCAATTCCAAGTTATCAGCAAGCTAATTTTCATGGCGTAACAAC
AAATCAGGCACAGAACTTAAACCCTTCCCAATTTCAAGCTGCCATGCAAC
CACCAGCAGATAAGGCAAATTTAGAGCCACAAAACCAAGCACTACGATTG
CAGCCTATGATCTCTGGGGATGGTCAGGGTACAACAGATGGGGAGGTCGA
TAAGAATCAGAGATACCAGTCAACACTACAATTTGCAGCAAACCTTCTTC
TCCAGATACAGCAGAAACAGCGGCAACAGTCTTCAGGTACTCCGGCTGGA
CAGGGGCCTTGAGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSMALSMK
PFRADDSGFQSNNLWVGSLTPETTESDLTELFGRYGDIDRITVYSSRGFA
FIYYRHVEEAVAAKEALQGANLNGSQIKIEYARPAKPCKSLWVGGIGPNV
SKDDLEEEFSKFGKIEDFRFLRERKTAFIDYYEMDDALQAKSMNGKPMGG
SFLRVDFLRSQAPKKEQWAGSYDNRNGNMNHKPQYPHSYEDFKGDVQPSK
VLWIGFPPTATQCNDEQILHNAMILFGEIERVKSYPSRNFALVEFRSAEE
ARQCKEGLQGRLFNNPRIKIMYSNDELPPEQDDTSFYSGMKRSRTDMFNN
DPSFVSSPHSTGIPGSMRPLRGTNERSYNGAEYNDVVGKEPNWRRPSANG
TGILPSPTGPGILPSPAQGTRRPMRSNPDSWEGYDPAQLVRESKRTRRDG
SVDGFTPMGVDERSFGRGSVAARPIRGPPDSDHIWRGMIAKGGTPVCCAR
CVPMGKGIETKLPEVVNCSARTDLNMLAKHYAVAIGCEIVFFVPDREEDF
ASYTEFLRYLSSKDRAGVAKLDDGTTLFLVPPSDFLTDVLQVTRQERLYG
VVLKLPPPAVPVTASYRQESQSNPLHYMDQARDSPANASHSLYPPRENYI
RGAPEHLTAASKPSVSEPLRIPNNAAPQAGVSLTPELLATLASILPATSQ
PAAPESHQPMSGPSTVVSTAHQSNGLYNGEAPSQAWKRGPQTVHDASNQS
FQQYGNQYTPAGQLPPPPSRYPPASNNPNYTSGMVHGNMQYQSQSVNMPQ
LSPLPNMPHNNYSMYTQGSSNHPVSQPMVQQYQPEASMPNQNYGPIPSYQ
QANFHGVTTNQAQNLNPSQFQAAMQPPADKANLEPQNQALRLQPMISGDG
QGTTDGEVDKNQRYQSTLQFAANLLLQIQQKQRQQSSGTPAGQGP*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16912
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence difference compared with NP_001078046.1 protein sequence:; Q889R