cDNA Clone NT5E

• Plasmid: pCMV5-HA NT5E
• Parent Plasmid: pCMV5 HA1
• Expressed: HA NT5E
• Genbank: AAH65937.1 GI:42406319
• Tag: HA
• Species: Human

Sequence of Insert

ggatccatgtgtccccgagccgcgcgggcgcccgcgacgctactcctcgc
cctgggcgcggtgctgtggcctgcggctggcgcctgggagcttacgattt
tgcacaccaacgacgtgcacagccggctggagcagaccagcgaggactcc
agcaagtgcgtcaacgccagccgctgcatgggtggcgtggctcggctctt
caccaaggttcagcagatccgccgcgccgaacccaacgtgctgctgctgg
acgccggcgaccagtaccagggcactatctggttcaccgtgtacaagggc
gccgaggtggcgcacttcatgaacgccctgcgctacgatgccatggcact
gggaaatcatgaatttgataatggtgtggaaggactgatcgagccactcc
tcaaagaggccaaatttccaattctgagtgcaaacattaaagcaaagggg
ccactagcatctcaaatatcaggactttatttgccatataaagttcttcc
tgttggtgatgaagttgtgggaatcgttggatacacttccaaagaaaccc
cttttctctcaaatccagggacaaatttagtgtttgaagatgaaatcact
gcattacaacctgaagtagataagttaaaaactctaaatgtgaacaaaat
tattgcactgggacattcgggttttgaaatggataaactcatcgctcaga
aagtgaggggtgtggacgtcgtggtgggaggacactccaacacatttctt
tacacaggcaatccaccttccaaagaggtgcctgctgggaagtacccatt
catagtcacttctgatgatgggcggaaggttcctgtagtccaggcctatg
cttttggcaaatacctaggctatctgaagatcgagtttgatgaaagagga
aacgtcatctcttcccatggaaatcccattcttctaaacagcagcattcc
tgaagatccaagcataaaagcagacattaacaaatggaggataaaattgg
ataattattctacccaggaattagggaaaacaattgtctatctggatggc
tcctctcaatcatgccgctttagagaatgcaacatgggcaacctgatttg
tgatgcaatgattaacaacaacctgagacacgcggatgaaacgttctgga
accacgtatccatgtgcattttaaatggaggtggtatccggtcgcccatt
gatgaacgcaacaatggcacaattacctgggagaacctggctgctgtatt
gccctttggaggcacatttgacctagtccagttaaaaggttccaccctga
agaaggcctttgagcatagcgtgcaccgctacggccagtccactggagag
ttcctgcaggtgggcggaatccatgtggtgtatgatctttcccgaaaacc
tggagacagagtagtcaaattagatgttctttgcaccaagtgtcgagtgc
ccagttatgaccctctcaaaatggacgaggtatataaggtgatcctccca
aacttcctggccaatggtggagatgggttccagatgataaaagatgaatt
attaagacatgactctggtgaccaagatatcaacgtggtttctacatata
tctccaaaatgaaagtaatttatccagcagttgaaggtcggatcaagttt
tccacaggaagtcactgccatggaagcttttctttaatatttctttcact
ttgggcagtgatctttgttttataccaatagcggccgc

Amino Acid Sequence

MYPYDVPDYAGSMCPRAARAPATLLLALGAVLWPAAGAWELTILHTNDVH
SRLEQTSEDSSKCVNASRCMGGVARLFTKVQQIRRAEPNVLLLDAGDQYQ
GTIWFTVYKGAEVAHFMNALRYDAMALGNHEFDNGVEGLIEPLLKEAKFP
ILSANIKAKGPLASQISGLYLPYKVLPVGDEVVGIVGYTSKETPFLSNPG
TNLVFEDEITALQPEVDKLKTLNVNKIIALGHSGFEMDKLIAQKVRGVDV
VVGGHSNTFLYTGNPPSKEVPAGKYPFIVTSDDGRKVPVVQAYAFGKYLG
YLKIEFDERGNVISSHGNPILLNSSIPEDPSIKADINKWRIKLDNYSTQE
LGKTIVYLDGSSQSCRFRECNMGNLICDAMINNNLRHADETFWNHVSMCI
LNGGGIRSPIDERNNGTITWENLAAVLPFGGTFDLVQLKGSTLKKAFEHS
VHRYGQSTGEFLQVGGIHVVYDLSRKPGDRVVKLDVLCTKCRVPSYDPLK
MDEVYKVILPNFLANGGDGFQMIKDELLRHDSGDQDINVVSTYISKMKVI
YPAVEGRIKFSTGSHCHGSFSLIFLSLWAVIFVLYQ*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16880
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Estimated MW of expressed protein : 64664 Daltons