cDNA Clone ATP1A1

• Plasmid: pGEX6P-3-ATP1A1
• Parent Plasmid: pGEX6P3
• Expressed: GST-ATP1A1
• Genbank: NM_000701.6 GI:48762680
• Tag: GST
• Species: Human

Sequence of Insert

GTCGACATGGGGAAGGGGGTTGGACGTGATAAGTATGAGCCTGCAGCTGT
TTCAGAACAAGGTGATAAAAAGGGCAAAAAGGGCAAAAAAGACAGGGACA
TGGATGAACTGAAGAAAGAAGTTTCTATGGATGATCATAAACTTAGCCTT
GATGAACTTCATCGTAAATATGGAACAGACTTGAGCCGGGGATTAACATC
TGCTCGTGCAGCTGAGATCCTGGCGCGAGATGGTCCCAACGCCCTCACTC
CCCCTCCCACTACTCCTGAATGGATCAAGTTTTGTCGGCAGCTCTTTGGG
GGGTTCTCAATGTTACTGTGGATTGGAGCGATTCTTTGTTTCTTGGCTTA
TAGCATCCAAGCTGCTACAGAAGAGGAACCTCAAAACGATAATCTGTACC
TGGGTGTGGTGCTATCAGCCGTTGTAATCATAACTGGTTGCTTCTCCTAC
TATCAAGAAGCTAAAAGTTCAAAGATCATGGAATCCTTCAAAAACATGGT
CCCTCAGCAAGCCCTTGTGATTCGAAATGGTGAGAAAATGAGCATAAATG
CGGAGGAAGTTGTGGTTGGGGATCTGGTGGAAGTAAAAGGAGGAGACCGA
ATTCCTGCTGACCTCAGAATCATATCTGCAAATGGCTGCAAGGTGGATAA
CTCCTCGCTCACTGGTGAATCAGAACCCCAGACTAGGTCTCCAGATTTCA
CAAATGAAAACCCCCTGGAGACGAGGAACATTGCCTTCTTTTCAACCAAT
TGTGTTGAAGGCACCGCACGTGGTATTGTTGTCTACACTGGGGATCGCAC
TGTGATGGGAAGAATTGCCACACTTGCTTCTGGGCTGGAAGGAGGCCAGA
CCCCCATTGCTGCAGAAATTGAACATTTTATCCACATCATCACGGGTGTG
GCTGTGTTCCTGGGTGTGTCTTTCTTCATCCTTTCTCTCATCCTTGAGTA
CACCTGGCTTGAGGCTGTCATCTTCCTCATCGGTATCATCGTAGCCAATG
TGCCGGAAGGTTTGCTGGCCACTGTCACGGTCTGTCTGACACTTACTGCC
AAACGCATGGCAAGGAAAAACTGCTTAGTGAAGAACTTAGAAGCTGTGGA
GACCTTGGGGTCCACGTCCACCATCTGCTCTGATAAAACTGGAACTCTGA
CTCAGAACCGGATGACAGTGGCCCACATGTGGTTTGACAATCAAATCCAT
GAAGCTGATACGACAGAGAATCAGAGTGGTGTCTCTTTTGACAAGACTTC
AGCTACCTGGCTTGCTCTGTCCAGAATTGCAGGTCTTTGTAACAGGGCAG
TGTTTCAGGCTAACCAGGAAAACCTACCTATTCTTAAGCGGGCAGTTGCA
GGAGATGCCTCTGAGTCAGCACTCTTAAAGTGCATAGAGCTGTGCTGTGG
TTCCGTGAAGGAGATGAGAGAAAGATACGCCAAAATCGTCGAGATACCCT
TCAACTCCACCAACAAGTACCAGTTGTCTATTCATAAGAACCCCAACACA
TCGGAGCCCCAACACCTGTTGGTGATGAAGGGCGCCCCAGAAAGGATCCT
AGACCGTTGCAGCTCTATCCTCCTCCACGGCAAGGAGCAGCCCCTGGATG
AGGAGCTGAAAGACGCCTTTCAGAACGCCTATTTGGAGCTGGGGGGCCTC
GGAGAACGAGTCCTAGGTTTCTGCCACCTCTTTCTGCCAGATGAACAGTT
TCCTGAAGGGTTCCAGTTTGACACTGACGATGTGAATTTCCCTATCGATA
ATCTGTGCTTTGTTGGGCTCATCTCCATGATTGACCCTCCACGGGCGGCC
GTTCCTGATGCCGTGGGCAAATGTCGAAGTGCTGGAATTAAGGTCATCAT
GGTCACAGGAGACCATCCAATCACAGCTAAAGCTATTGCCAAAGGTGTGG
GCATCATCTCAGAAGGCAATGAGACCGTGGAAGACATTGCTGCCCGCCTC
AACATCCCAGTCAGCCAGGTGAACCCCAGGGATGCCAAGGCCTGCGTAGT
ACACGGCAGTGATCTAAAGGACATGACCTCCGAGCAGCTGGATGACATTT
TGAAGTACCACACTGAGATAGTGTTTGCCAGGACCTCCCCTCAGCAGAAG
CTCATCATTGTGGAAGGCTGCCAAAGACAGGGTGCTATCGTGGCTGTGAC
TGGTGACGGTGTGAATGACTCTCCAGCTTTGAAGAAAGCAGACATTGGGG
TTGCTATGGGGATTGCTGGCTCAGATGTGTCCAAGCAAGCTGCTGACATG
ATTCTTCTGGATGACAACTTTGCCTCAATTGTGACTGGAGTAGAGGAAGG
TCGTCTGATCTTTGATAACTTGAAGAAATCCATTGCTTATACCTTAACCA
GTAACATTCCCGAGATCACCCCGTTCCTGATATTTATTATTGCAAACATT
CCACTACCACTGGGGACTGTCACCATCCTCTGCATTGACTTGGGCACTGA
CATGGTTCCTGCCATCTCCCTGGCTTATGAGCAGGCTGAGAGTGACATCA
TGAAGAGACAGCCCAGAAATCCCAAAACAGACAAACTTGTGAATGAGCGG
CTGATCAGCATGGCCTATGGGCAGATTGGAATGATCCAGGCCCTGGGAGG
CTTCTTTACTTACTTTGTGATTCTGGCTGAGAACGGCTTCCTCCCAATTC
ACCTGTTGGGCCTCCGAGTGGACTGGGATGACCGCTGGATCAACGATGTG
GAAGACAGCTACGGGCAGCAGTGGACCTATGAGCAGAGGAAAATCGTGGA
GTTCACCTGCCACACAGCCTTCTTCGTCAGTATCGTGGTGGTGCAGTGGG
CCGACTTGGTCATCTGTAAGACCAGGAGGAATTCGGTCTTCCAGCAGGGG
ATGAAGAACAAGATCTTGATATTTGGCCTCTTTGAAGAGACAGCCCTGGC
TGCTTTCCTTTCCTACTGCCCTGGAATGGGTGTTGCTCTTAGGATGTATC
CCCTCAAACCTACCTGGTGGTTCTGTGCCTTCCCCTACTCTCTTCTCATC
TTCGTATATGACGAAGTCAGAAAACTCATCATCAGGCGACGCCCTGGCGG
CTGGGTGGAGAAGGAAACCTACTATTAGCGGCCGC

Amino Acid Sequence

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL
EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL
DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTH
PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA
WPLQGWQATFGGGDHPPKSDLEVLFQGPLGSPNSRVDMGKGVGRDKYEPA
AVSEQGDKKGKKGKKDRDMDELKKEVSMDDHKLSLDELHRKYGTDLSRGL
TSARAAEILARDGPNALTPPPTTPEWIKFCRQLFGGFSMLLWIGAILCFL
AYSIQAATEEEPQNDNLYLGVVLSAVVIITGCFSYYQEAKSSKIMESFKN
MVPQQALVIRNGEKMSINAEEVVVGDLVEVKGGDRIPADLRIISANGCKV
DNSSLTGESEPQTRSPDFTNENPLETRNIAFFSTNCVEGTARGIVVYTGD
RTVMGRIATLASGLEGGQTPIAAEIEHFIHIITGVAVFLGVSFFILSLIL
EYTWLEAVIFLIGIIVANVPEGLLATVTVCLTLTAKRMARKNCLVKNLEA
VETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDK
TSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELC
CGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPER
ILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDE
QFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKV
IMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKAC
VVHGSDLKDMTSEQLDDILKYHTEIVFARTSPQQKLIIVEGCQRQGAIVA
VTGDGVNDSPALKKADIGVAMGIAGSDVSKQAADMILLDDNFASIVTGVE
EGRLIFDNLKKSIAYTLTSNIPEITPFLIFIIANIPLPLGTVTILCIDLG
TDMVPAISLAYEQAESDIMKRQPRNPKTDKLVNERLISMAYGQIGMIQAL
GGFFTYFVILAENGFLPIHLLGLRVDWDDRWINDVEDSYGQQWTYEQRKI
VEFTCHTAFFVSIVVVQWADLVICKTRRNSVFQQGMKNKILIFGLFEETA
LAAFLSYCPGMGVALRMYPLKPTWWFCAFPYSLLIFVYDEVRKLIIRRRP
GGWVEKETYY*

• Vector Type: Bacterial
• Antibiotic: Amp
• DU Number: DU16865
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Bacterial construct to express GST N terminal fusion.; ; Estimated MW of expressed protein : 140383 Daltons