cDNA Clone MICALL1

• Plasmid: pcDNA5-FRT/TO-GFP-TAP MICALL1
• Parent Plasmid: pcDNA5 FRT/TO GFPTAP
• Expressed: GFPTAP MICALL1
• Genbank: NM_033386.2 GI:51317359*
• Tag: GFP
• Tag: TAP
• Species: Human

Sequence of Insert

AGATCTATGGCTGGGCCGCGGGGCGCGCTGCTGGCCTGGTGCCGCCGCCA
GTGCGAGGGCTACCGCGGCGTGGAGATCCGCGACCTGAGCAGCTCCTTCC
GGGACGGCCTGGCCTTCTGCGCCATCCTGCACCGGCACCGGCCCGACCTG
CTAGATTTTGATTCGCTTTCCAAGGACAATGTCTTCGAGAATAACCGTTT
GGCCTTTGAAGTGGCTGAGAAGGAGCTGGGGATCCCCGCTCTCCTGGACC
CCAATGACATGGTCTCCATGAGCGTCCCTGACTGCCTCAGCATCATGACC
TATGTGTCCCAGTATTACAACCACTTCTGCAGTCCTGGCCAAGCTGGTGT
CTCGCCACCCAGAAAGGGTCTTGCACCCTGTTCCCCGCCGTCTGTAGCAC
CCACTCCAGTGGAACCAGAAGATGTGGCTCAGGGCGAGGAGCTCTCCTCA
GGCAGCCTGTCAGAGCAGGGCACCGGCCAGACCCCCAGCAGCACGTGCGC
AGCCTGCCAGCAGCATGTGCACTTGGTGCAGCGCTACCTGGCTGACGGCA
GGCTGTACCATCGCCACTGCTTCCGGTGTCGGCGGTGCTCCAGCACCCTG
CTCCCTGGGGCTTATGAGAATGGGCCTGAGGAGGGCACCTATGTGTGTGC
AGAACACTGTGCCAGGCTGGGCCCGGGGACACGGTCGGGGACCAGGCCTG
GGCCCTTCTCACAGCCAAAGCAGCAGCACCAGCAGCAACTCGCAGAAGAT
GCCAAGGATGTTCCAGGAGGCGGCCCCAGCTCCAGTGCTCCTGCAGGGGC
TGAGGCCGATGGACCCAAGGCCAGCCCTGAGGCCCGGCCGCAGATCCCTA
CCAAGCCCCGGGTTCCTGGCAAACTACAGGAGCTGGCCAGCCCCCCTGCG
GGCCGCCCCACCCCTGCCCCCAGGAAGGCCTCTGAGAGCACCACCCCAGC
ACCCCCCACGCCCCGGCCCCGCTCCAGTCTGCAGCAGGAGAACCTGGTGG
AGCAGGCTGGCAGCAGCAGCCTGGTGAACGGGAGACTGCACGAACTGCCT
GTCCCCAAGCCGAGGGGGACACCGAAGCCGTCCGAGGGGACACCAGCCCC
CAGGAAGGACCCCCCATGGATCACGCTGGTGCAGGCAGAACCAAAGAAGA
AGCCAGCCCCACTTCCCCCAAGCAGCAGCCCGGGGCCACCAAGCCAGGAC
AGCAGGCAGGTGGAGAATGGAGGCACCGAGGAGGTGGCCCAGCCGAGCCC
AACGGCCAGCCTGGAGTCCAAACCCTATAACCCCTTTGAGGAGGAGGAGG
AGGACAAGGAGGAAGAGGCTCCAGCTGCACCCAGCCTGGCCACCAGCCCT
GCCCTGGGCCACCCGGAGTCCACACCCAAGTCCCTGCACCCCTGGTACGG
CATCACCCCTACCAGCAGCCCCAAGACAAAGAAGCGCCCTGCCCCGCGCG
CACCCAGCGCGTCCCCACTGGCTCTCCACGCCTCCCGCCTCTCGCACTCG
GAGCCGCCCTCGGCCACACCATCGCCAGCGCTCAGCGTGGAGAGCCTGTC
GTCTGAGAGCTCCAGCCAGACTGCAGGTGCAGAGCTTCTGGAGCCGCCAG
CTGTGCCCAAGAGCTCCTCAGAGCCTGCTGTCCATGCCCCTGGTACCCCT
GGAAACCCTGTCAGCCTCTCTACCAACTCCTCCCTGGCCTCCTCTGGGGA
ACTAGTGGAGCCTAGAGTGGAACAAATGCCTCAAGCCAGCCCTGGCCTTG
CCCCCAGGACCAGGGGCAGCTCAGGTCCCCAGCCAGCCAAGCCCTGCAGT
GGCGCCACCCCAACGCCTCTCTTGTTGGTTGGAGACAGGAGCCCGGTGCC
TTCCCCTGGAAGCTCGTCCCCACAGCTGCAGGTAAAGTCCTCCTGCAAGG
AGAATCCTTTTAACCGGAAGCCATCACCTGCAGCGTCCCCAGCCACAAAG
AAGGCCACCAAGGGATCCAAGCCAGTGAGGCCACCTGCCCCTGGACACGG
CTTTCCACTCATCAAACGCAAGGTCCAGGCTGACCAGTACATCCCTGAGG
AGGACATCCATGGAGAGATGGATACCATTGAGCGCCGGCTGGATGCCCTG
GAGCACCGTGGGGTGCTGCTGGAGGAGAAGCTGCGTGGCGGCCTGAATGA
GGGCCGTGAGGATGACATGCTGGTGGACTGGTTCAAGCTCATCCACGAGA
AGCACCTACTGGTGCGGCGAGAGTCCGAGCTCATCTATGTCTTCAAGCAG
CAGAACCTGGAGCAGCGCCAGGCTGATGTCGAGTATGAGCTCCGGTGCCT
CCTCAATAAGCCAGAAAAGGACTGGACGGAGGAGGACCGGGCCCGGGAGA
AGGTGCTGATGCAGGAGCTTGTGACCCTCATTGAGCAGCGCAACGCTATC
ATCAACTGCCTGGATGAGGACCGGCAGAGGGAGGAAGAGGAAGACAAGAT
GTTGGAAGCCATGATCAAGAAGAAAGAGTTCCAGAGGGAGGCTGAACCTG
AGGGCAAGAAGAAGGGGAAGTTCAAGACCATGAAGATGTTGAAACTGCTA
GGAAACAAACGTGATGCCAAGAGCAAGTCCCCCAGAGACAAGAGCTAAGC
GGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGRTQISSSSF
DIALATMDTSAHDEAVDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLK
DDPSQSANLLAEAKKLNDAQAPKVDNKFNKERQNAFYEILHLPNLNEEQR
NAFIQSLKDDPSQSANLLAEAKKLNDAQAPKVDASWSAGASENLYFQGGR
SMEKRRWKKNFIAVSAANRFKKISSSGALDYDIPTALEVLFQGPGSMAGP
RGALLAWCRRQCEGYRGVEIRDLSSSFRDGLAFCAILHRHRPDLLDFDSL
SKDNVFENNRLAFEVAEKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYY
NHFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDVAQGEELSSGSLSEQ
GTGQTPSSTCAACQQHVHLVQRYLADGRLYHRHCFRCRRCSSTLLPGAYE
NGPEEGTYVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQLAEDAKDVPG
GGPSSSAPAGAEADGPKASPEARPQIPTKPRVPGKLQELASPPAGRPTPA
PRKASESTTPAPPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVPKPRG
TPKPSEGTPAPRKDPPWITLVQAEPKKKPAPLPPSSSPGPPSQDSRQVEN
GGTEEVAQPSPTASLESKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPE
STPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLALHASRLSHSEPPSAT
PSPALSVESLSSESSSQTAGAELLEPPAVPKSSSEPAVHAPGTPGNPVSL
STNSSLASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAKPCSGATPTP
LLLVGDRSPVPSPGSSSPQLQVKSSCKENPFNRKPSPAASPATKKATKGS
KPVRPPAPGHGFPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEHRGVL
LEEKLRGGLNEGREDDMLVDWFKLIHEKHLLVRRESELIYVFKQQNLEQR
QADVEYELRCLLNKPEKDWTEEDRAREKVLMQELVTLIEQRNAIINCLDE
DRQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFKTMKMLKLLGNKRDA
KSKSPRDKS*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16717
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee
• Notes: Sequence differences compared with NM_033386.2 CDS:; c363t (silent), t635a (F212Y), g1555t (A519S) - present in other published seqs