cDNA Clone GRIPE

• Plasmid: pcDNA5-FRT/TO-GFP GRIPE H1147-A2034
• Parent Plasmid: pcDNA5 FRT/TO GFP
• Expressed: GFP GRIPE H1147-A2034
• Genbank: NM_194301.2 GI:51230411
• Tag: GFP
• Species: Human

Sequence of Insert

GGATCCcatgcatataaacttatttgtaatacaatgaaaagaagacaagatgtttctccaaatagagattttctaacacatttctacaatataatgcattgtggattacttcatattgaccaggatattgtcaatacaatcatcaaacactgctcacctcaatttttttcacttggtttgcctggtgccacaatgcttattatggattttattgtagcagctggtagagtggcttcttcagcttttctcaatgcaccaagagtagaagcacaagttcttctgggatctttggtttgctttcccaac
ttatattgtgaactgccttctcttcatcccaacattcctgatgttgctgt
gtctcagtttacagatgttaaggaacttataatcaaaactgtattaagct
cggcaagagatgagccctctggtcctgcacgatgtgtagcactttgtagt
ttaggtatttggatttgtgaagaactagtccatgagtctcatcatcctca
aattaaggaagctctgaatgtgatttgtgtttccttaaagtttactaata
aaacagtagcccacgtagcttgtaacatgcttcacatgctggttcattat
gtacctagacttcagatttaccagcctgattctcccttgaaaattattca
aatcctaatagctaccatcacccatcttttaccaagtacagaggcttcat
cttatgaaatggacaagaggttggtagtatctttacttctctgccttctg
gactggatcatggccttacctctaaagacactgctccaaccatttcatgc
tacgggagcagaaagcgataaaacagaaaaatctgttctcaattgcattt
ataaggttttacatgggtgtgtttatggagctcagtgttttagcaatcca
aggtattttcccatgagcctctctgatttggcatctgtagattatgatcc
ttttatgcatttggaaagtctgaaagagcctgagcctctgcactctcctg
actcagaacgatcttctaaactccagccagtaacagaagtgaaaactcaa
atgcagcatggattaatctctatagcagcccgcactgttattacacatct
ggtaaatcacctgggccattatccaatgagcggtggtcctgctatgctaa
caagtcaggtgtgtgaaaatcacgacaatcattacagtgaaagtactgaa
ctttctcctgaactctttgagagtccaaatatccagttctttgtgttaaa
taatacaaccttagtgtcctgtatccagatcagatcagaagagaatatgc
ctggaggaggtttatctgctggccttgcatcagccaattcaaatgtcaga
atcatagtacgtgatctctctggaaaatattcatgggattctgctatact
gtatggcccacctcctgtaagtggcttgtcagaacctacatctttcatgc
tttcattgtctcaccaagagaagccagaagagcctccgacatctaatgaa
tgcttagaagatataaccgtaaaagatggactttctctccagtttaaaag
atttagagaaactgtaccaacttgggatacaataagagatgaagaagatg
ttcttgatgagctcttgcagtatttgggtgttactagtcctgaatgctta
cagagaactggaatctcacttaatattcctgctccacaacctgtgtgcat
ttctgaaaaacaagaaaatgatgttattaatgctatccttaagcaacata
cagaagaaaaagaatttgttgagaagcactttaatgacttaaacatgaaa
gctgtggaacaagatgaaccaatacctcaaaaacctcagtcagcatttta
ttattgcagattgcttcttagtatattgggaatgaattcctgggacaaac
ggaggagctttcatctcctgaagaaaaatgaaaagctacttagagaactt
aggaacttggattcaaggcagtgccgagagacacacaagattgcagtatt
ttatgttgctgaaggacaagaagacaaacactccattctcaccaatacag
gaggaagtcaagcatatgaagattttgtagctggtcttggttgggaggta
aatcttacaaaccattgtggttttatgggaggactacaaaaaaacaaaag
cactggattgaccactccatattttgctacctctacagtagaggtaatat
ttcacgtgtcaacaagaatgccttctgattctgatgattctttgaccaaa
aaattgagacatttgggaaatgatgaagtgcacattgtttggtcagagca
tactagagactacaggagaggaattattcccacagaatttggtgatgtcc
ttattgtaatatatccaatgaaaaatcacatgttcagtattcagataatg
aaaaaaccagaggttcccttctttggtcccctttttgatggtgctattgt
gaatggaaaggttctacccattatggttagagcaacagctataaatgcaa
gccgtgctctgaaatctctgattccattgtatcaaaacttctatgaggag
agagcacgatacctgcaaacaattgtccagcaccacttagaaccaacaac
atttgaagattttgcagcacaggttttttctccagctccctaccaccatt
taccatctgatgccgATCATTAAATATCAGTTCTGTTTATCTGAAGgctc
ctacccagagattctacccagtgaaactcccacagcaacgcaggtagatg
gggctgacctggcctctccaatgtctcctcgaactagcaaaagccgcatg
tccatgaagctgcgtcgttcctctggctcagccaataaatcctaaGCGGCCGC

Amino Acid Sequence

MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT
TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF
FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHN
VYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKSGLGSHAYKLI
CNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNTIIKHCSPQFFSL
GLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELP
SLHPNIPDVAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWIC
EELVHESHHPQIKEALNVICVSLKFTNKTVAHVACNMLHMLVHYVPRLQI
YQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIMAL
PLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVYGAQCFSNPRYFPMS
LSDLASVDYDPFMHLESLKEPEPLHSPDSERSSKLQPVTEVKTQMQHGLI
SIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNHYSESTELSPELF
ESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRIIVRDL
SGKYSWDSAILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDIT
VKDGLSLQFKRFRETVPTWDTIRDEEDVLDELLQYLGVTSPECLQRTGIS
LNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQDE
PIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSR
QCRETHKIAVFYVAEGQEDKHSILTNTGGSQAYEDFVAGLGWEVNLTNHC
GFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDSDDSLTKKLRHLG
NDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVP
FFGPLFDGAIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQ
TIVQHHLEPTTFEDFAAQVFSPAPYHHLPSDADH*

• Vector Type: Mammalian
• Antibiotic: Amp
• DU Number: DU16654
• Unit Source: Protein Phosphorylation and Ubiquitylation Unit
• Location: PPU
• MTA: Dundee